The purpose is to demonstrate the optical charactering concerning nasopharyngeal tissue of pig by fresh sections and frozen correlating sections with optical coherence tomography (OCT). After being imaged on a fresh specimen, samples are then stored in low temperature refrigerators ($-80°\mathrm{C}$) for one year for the second OCT measurement. The OCT structure of the epithelium, lamina propria, and the basement membrane are still resolvable; the median scattering coefficients and anisotropy factors fitting from OCT images based on the multiple scattering effects for epithelium are $27.6{\mathrm{mm}}^{-1}$ [interquartile range (IQR) 23.6 to $29.3{\mathrm{mm}}^{-1}$] versus $22.5{\mathrm{mm}}^{-1}$ (IQR 20.5 to $24.4{\mathrm{mm}}^{-1}$), 0.86 (IQR 0.81 to 0.9) versus 0.88 (IQR 0.87 to 0.9) for fresh and frozen tissue, respectively; and $10.2{\mathrm{mm}}^{-1}$ (IQR 8.1 to $13.6{\mathrm{mm}}^{-1}$) versus $9.6{\mathrm{mm}}^{-1}$ (IQR 8.1 to $13.8{\mathrm{mm}}^{-1}$), 0.96 (IQR 0.93 to 0.98) versus 0.92 (IQR 0.9 to 0.98) for lamina propria, respectively. The results show that the frozen storage method can be used for OCT research.