Two dimensional materials beyond graphene such as MoS₂ and WS₂ are novel and interesting class of materials whose unique physico-chemical properties can be exploited in applications ranging from leading edge nanoelectronics to the frontiers between biomedicine and biotechnology. To unravel the potential of TMD crystals in biomedicine, control over their production through green and scalable routes in biocompatible solvents is critically important. Furthermore, considering multiple applications of eco-friendly 2D dispersions and their potential impact onto live matter, their toxicity and antimicrobial activity still remain an open issue. Herein, we focus on the current demands of 2D TMDs and produce high-quality, few-layered and defect-free MoS₂ nanosheets, exfoliated and dispersed in pure water, stabilized up to three weeks. Hence, we studied the impact of this material on human cells by investigating its interactions with three cell lines: two tumoral, MCF7 (breast cancer) and U937 (leukemia), and one normal, HaCaT (epithelium). We observed novel and intriguing results, exhibiting evident cytotoxic effect induced in the tumor cell lines, absent in the normal cells in the tested conditions. The antibacterial action of MoS₂ nanosheets is then investigated against a very dangerous gram negative bacterium, such as two types of Salmonellas: ATCC 14028 and wild-type Salmonella typhimurium. Additionally, concentration and layer-dependent modulation of cytotoxic effect is found both on human cells and Salmonellas.

Interactions of novel bi-dimensional nanomaterials and live matter such as bacteria and viruses represent an extremely hot topic due to the unique properties of the innovative nanomaterials, capable in some cases to exhibit bactericide and antiviral actions. The interactions between bacteria and viruses and two dimensional nanosheets are here investigated. We extensively studied the interaction between a gram-negative bacterium, Escherichia coli, and a gram-positive bacterium, Staphylococcus aureus, with two different types of 2D nanoflakes such as MoS₂, belonging to the Transition Metal Dichalcogenides family, and Graphene Oxide. The same two types of nanomaterials were employed to study their antiviral action toward the Herpes simplex virus type-1, (HSV-1). The experimental results showed different bactericide impacts as well as different antiviral power between the two nanomaterials. The experimental findings were interpreted in bacteria on the base of the Derjaguin–Landau–Verwey–Overbeek theory. A simple kinetic model of bacterial growth in the presence of the interacting nanosheets is also elaborated, to explain the observed results. The experimental results in viruses are really novel and somewhat surprising, evidencing a stronger antiviral action of Graphene Oxide as compared to MoS₂. Results in viruses are complicated to quantitatively interpret due to the complexity of the system under study, constituted by virus/host cell and nanoflake, and due to the lack of a well assessed theoretical context to refer to. Thus, these results are interpreted in terms of qualitative arguments based on the chemical properties of the interactors in the given solvent medium.

We describe a very simple physical model that allows the analysis of high-order harmonic generation in gases when the pumping laser beam has an intensity profile that is not Gaussian but truncated Bessel. This is the typical experimental condition when sub-10-fs pump-laser pulses, generated by the hollow fiber compression technique, are used. This model is based on the analysis of the phase-matching conditions for the harmonic generation process revisited in view of the new spatial mode of the fundamental beam. In particular, the role of the atomic dipole phase and the geometric phase terms are evidenced both for harmonics generated in the plateau and in the cutoff spectral regions. The influence of dispersion introduced by free electrons produced by laser ionization has also been discussed in some detail. Spatial patterns of far-field harmonics are then obtained by means of a simplified algorithm which allows one to avoid the numerical integration of the harmonic beam propagation equation. Experimental spatial distributions and divergence angles of high-order harmonics generated in Ne with 7-fs titanium-sapphire pulses are compared with numerical simulations in various experimental conditions. The agreement between measurements and calculated results is found to be very satisfactory.

High harmonic generation provides a means of producing attosecond pulses of light which are the shortest, controllable probes available to science for time-resolving ultrafast dynamics. We review techniques based on high harmonic generation for generating single attosecond pulses using high-power, multi-cycle laser sources, including optical-, polarisation-, and ionisation-gating schemes as well as techniques based on field synthesis. By significantly reducing the technical demands placed on the driving laser, these techniques have the potential to greatly broaden the application base for attosecond pulses.

A monolithic silica gel matrix with entrapped glucose oxidase (GOD) was constructed as a bioactive element in an optical biosensor for glucose determination. Intrinsic fluorescence of free and immobilised GOD was investigated in the visible range in presence of different glucose concentrations by time-resolved spectroscopy with time-correlated single-photon counting detector. A three-exponential model was used for analysing the fluorescence transients. Fractional intensities and mean lifetime were shown to be sensitive to the enzymatic reaction and were used for obtaining calibration curve for glucose concentration determination. The sensing system proposed achieved high resolution (up to 0:17 mM) glucose determination with a detection range from 0:4 mM to 5 mM.

Green synthesis of MoS₂/biofunctionalized graphene hybrid films assisted by Vmh2 hydrophobin for applications in biosensing and photodetection.