Wildland fires represent a major threat to Quercus suber L. ecosystems, which provide relevant socioeconomic and ecological services in the Mediterranean Basin. In this work, we analyzed recent wildland fire dynamics in cork oak woodlands along the fire-prone areas of Sardinia (Italy), Corsica (France) and Catalonia (Spain). We first characterized geographic extent and main characteristics of cork oak woodlands in these regions and analyzed how environmental (climate and elevation) and socioeconomic factors (population and land uses) vary in the areas covered by Quercus suber L. We then evaluated how wildfires affected cork oak stands and, by logistic regression analysis, to what extent wildfires in cork oak areas were related to the above set of environmental and anthropic explanatory variables. Results revealed specific variations across study areas in cork oak characteristics as well as in environmental and social factors. We highlighted the spatial and temporal patterns of wildfires on cork oak woodlands, in terms of extent, seasonality, frequency and main driving factors. In the period 2003–2015, the percentage of cork oak woodlands burned ranged from 3.42% in Corsica to 11.30% in Sardinia. A few large and severe wildfires accounted for most of the area burned in cork oak woodlands. The most significant predictive variable that explained the spatial variation in wildland fire ignitions inside or nearby cork oaks was summer precipitation, while the weight of other factors varied depending on the region. This study provides evidence on recent fire dynamics in cork oak woodlands and gives valuable information and insights for the implementation of forest management and planning strategies in the Mediterranean area.

Bacterial diversity of 15 extra virgin olive oils, obtained from different Italian varieties, including Frantoio, Coratina, Bosana, and Semidana, was analyzed in this study. All bacterial isolates were genotyped using RAPD and REP-PCR method and grouped by means of cluster analyses. Sequencing of 16S rDNA of 51 isolates, representative of 36 clusters, led to the identification of Bacillus spp., Brevibacillus spp., Micrococcus spp., Staphylococcus spp., Pantoea spp., Kocuria spp., Lysinbacillus spp., and Lactobacillus spp., most of which reported for first time in olive oils. Phenotypic characterization of the 51 isolates, some of which ascribed to potentially probiotic species, indicate that two of them have beta-glucosidase activity while 37% present lipolytic activity. Preliminary evaluation of probiotic potential indicates that 31% of the isolates show biofilm formation ability, 29% acidic pH resistance, and 25% bile salt resistance. Finally, 29% of the isolates were sensitive to antibiotics while the remaining 71%, that include bacterial species well-recognized for their ability to disseminate resistance genes in the environment, showed a variable pattern of antibiotic resistance. The results obtained underline that microbial diversity of extra virgin olive oils represents an unexpected sink of microbial diversity and poses safety issues on the possible biotechnological exploitation of this microbial biodiversity.

Recently, significant forestry activities have taken place in Sardinia thanks to EU Regulation 2080/92 funds. Some 80% of the afforestated surface has been planted with holm oak and cork oak. The latter also characterizes 89% of the reforestation area. Given the funding source, plantations have been established on farmlands. Growth performances of these recent cork oak stands have been quantitatively evaluated and compared with the performances of two experimental plots. In Gallura (north-east of Sardinia), that is the traditional cork production area and still is economically the most important cork district of the island, these new cork oak plantations have an average size of 28 ha. They have been established on lands that, before plantation, were either pastures (30%) or arable lands (70%). Plantation failures are limited to 8.8% of the total (in term of mass) and seem independent of environmental factors or plantations species composition (conifers have been frequently used as secondary species). Average growth of the stem, measured above cork at collar height, is in the range 4 to 8mm/year with a mean value of 5.5mm/year. No correlation appears with either environmental conditions or species composition of the plantations. In the first experimental plot, soil management practices (natural vegetation removal vs. its cutting and mulching) does not differentiate young plants growth trends. In the control subplots (no removal) stem collar diameter is 20% smaller. Localized manual hoeing around trunk base increased the diameters by 13% but reduced cork thickness by 21%. The second experimental plot allows comparisons among 27 Mediterranean proveniences of cork oak. The trial exhibits reduced genetic influence: diameters and heights growth are significantly different only among extreme groups. In conclusion, reduced growth performances of the plantations established in farmlands is due, to some extent, to the limitations inherent with private land management (constrained to costs minimization) and, on the other hand, to the generally very limited thickness of Gallura soils. Demand for good quality commercial cork is steadily raising. To sustain the request, in the short term, it would be necessary to extend financial support for plantations care, from 5 to 10 years, or even up to the first stripping (virgin cork).

Due to its high sensitivity to numerous variability sources, it is hard to define the typicity of a monovarietal virgin olive oil (VOO) according to its phenolic profile. In this study, we aimed to identify the features of phenolic composition that are persistent and minimally affected by variability sources, making them potential varietal markers. We separately analyzed three databases of monovarietal VOO phenolic compositions, determined by liquid chromatography, from three different cultivars. The first database was produced from the original data of the Bosana cultivar. The other two were obtained through a systematic analysis of scientific literature on Coratina and Frantoio cultivars. Several statistical tools, including coefficient of variability, correlations, and linear regression models, were used to find recurring proportions or ratios unaffected by variability sources suitable to define typical varietal traits. Some proportions between molecules, mostly within the same phenolic class, remain constant. Strong correlations between (i) flavonoids were observed in Bosana and Frantoio VOOs (R² = 0.87 and 0.77, respectively), (ii) oleacein-oleocanthal (Bosana, R² = 0.81) (iii) oleuropein aglycon-ligstroside aglycon (Frantoio, R² = 0.88), and (iv) lignans (Coratina, R² = 0.84). These traits could be useful tools for defining the typicity of monovarietal VOOs.