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The induction of a specific antibody response has long been accepted as a serological hallmark of recent infection or antigen exposure. Much of our understanding of the influenza antibody response has been derived from studying antibodies that target the hemagglutinin (HA) protein. However, growing evidence points to limitations associated with this approach. In this review, we aim to highlight the issue of antibody non-responsiveness after influenza virus infection and vaccination. We will then provide an overview of the major factors known to influence antibody responsiveness to influenza after infection and vaccination. We discuss the biological factors such as age, sex, influence of prior immunity, genetics, and some chronic infections that may affect the induction of influenza antibody responses. We also discuss the technical factors, such as assay choices, strain variations, and viral properties that may influence the sensitivity of the assays used to measure influenza antibodies. Understanding these factors will hopefully provide a more comprehensive picture of what influenza immunogenicity and protection means, which will be important in our effort to improve influenza vaccines.
Xia Lin; Fangmei Lin; Tingting Liang; Mariette Ducatez; Mark Zanin; Sook-San Wong. Antibody Responsiveness to Influenza: What Drives It? Viruses 2021, 13, 1400 .
AMA StyleXia Lin, Fangmei Lin, Tingting Liang, Mariette Ducatez, Mark Zanin, Sook-San Wong. Antibody Responsiveness to Influenza: What Drives It? Viruses. 2021; 13 (7):1400.
Chicago/Turabian StyleXia Lin; Fangmei Lin; Tingting Liang; Mariette Ducatez; Mark Zanin; Sook-San Wong. 2021. "Antibody Responsiveness to Influenza: What Drives It?" Viruses 13, no. 7: 1400.
Genetic analysis of circulating avian influenza viruses (AIVs) in wild birds at different geographical regions during the same period could improve our knowledge about virus transmission dynamics in natural hosts, virus evolution as well as zoonotic potential. Here, we report the genetic and molecular characterization of H6N2 influenza viruses isolated from migratory birds in Turkey, Egypt, and Uganda during 2017–2018. The Egyptian and Turkish isolates were genetically closer to each other than they were to the virus isolated from Uganda. Our results also suggest that multiple reassortment events were involved in the genesis of the isolated viruses. All viruses contained molecular markers previously associated with increased replication and/or pathogenicity in mammals. The results of this study indicate that H6N2 viruses carried by migratory birds on the West Asian/East African and Mediterranean/Black Sea flyways have the potential to transmit to mammals including humans. Additionally, adaptation markers in these viruses indicate the potential risk for poultry, which also increases the possibility of human exposure to these viruses.
Yavuz Mercan; Gladys Atim; Ahmed Kayed; M. Azbazdar; Ahmed Kandeil; Mohamed Ali; Adam Rubrum; Pamela McKenzie; Richard Webby; Bernard Erima; Fred Wabwire-Mangen; Qouilazoni Ukuli; Titus Tugume; Denis Byarugaba; Ghazi Kayali; Mariette Ducatez; Zeynep Koçer. Molecular Characterization of Closely Related H6N2 Avian Influenza Viruses Isolated from Turkey, Egypt, and Uganda. Viruses 2021, 13, 607 .
AMA StyleYavuz Mercan, Gladys Atim, Ahmed Kayed, M. Azbazdar, Ahmed Kandeil, Mohamed Ali, Adam Rubrum, Pamela McKenzie, Richard Webby, Bernard Erima, Fred Wabwire-Mangen, Qouilazoni Ukuli, Titus Tugume, Denis Byarugaba, Ghazi Kayali, Mariette Ducatez, Zeynep Koçer. Molecular Characterization of Closely Related H6N2 Avian Influenza Viruses Isolated from Turkey, Egypt, and Uganda. Viruses. 2021; 13 (4):607.
Chicago/Turabian StyleYavuz Mercan; Gladys Atim; Ahmed Kayed; M. Azbazdar; Ahmed Kandeil; Mohamed Ali; Adam Rubrum; Pamela McKenzie; Richard Webby; Bernard Erima; Fred Wabwire-Mangen; Qouilazoni Ukuli; Titus Tugume; Denis Byarugaba; Ghazi Kayali; Mariette Ducatez; Zeynep Koçer. 2021. "Molecular Characterization of Closely Related H6N2 Avian Influenza Viruses Isolated from Turkey, Egypt, and Uganda." Viruses 13, no. 4: 607.
Background Influenza D virus (IDV), a segmented single stranded negative sense ribonucleic acid (RNA) virus belongs to the new Delta influenza virus genus of the Orthomyxoviridae family. Cattle was proposed as the natural reservoir of IDV in which infection was associated with mild to moderate respiratory clinical signs (i.e. cough, nasal discharge, and dyspnoea). Methods and principal findings In order to investigate the role of IDV in bovine respiratory disease, during the period 2017‐2020, 883 nasal or naso‐pharyngeal swabs from Canadian cattle with respiratory signs (cough and/or dyspnoea) were tested by (RT‐)qPCR for IDV and other major bovine viral (bovine herpesvirus 1, bovine viral diarrhoea virus, bovine respiratory syncytial virus, bovine parainfluenza virus 3, and bovine coronavirus) and bacterial (Mannheimia haemolytica, Pasteurella multocida, Histophilus somni, and Mycoplasma bovis) respiratory pathogens. In addition, whole genome sequencing and phylogenetic analyses were carried out on five IDV positive samples. The prevalence of IDV RT‐qPCR (with cut‐off: Cq < 38) at animal level was estimated at 5.32% (95% confidence interval: 3.94‐7.02). Positive result of IDV was significantly associated with (RT‐)qPCR positive results for bovine respiratory syncytial virus and Mycoplasma bovis. While phylogenetic analyses indicate that most segments belonged to clade D/660, reassortment between clades D/660 and D/OK were evidenced in four samples collected in 2018‐2020. Conclusions and significance Relative importance of influenza D virus and associated pathogens in bovine respiratory disease of Canadian dairy cattle was established. Whole genome sequencing demonstrated evidence of reassortment between clades D/660 and D/OK. Both these new pieces of information claim for more surveillance of IDV in cattle production worldwide.
Claude Saegerman; Maria Gaudino; Christian Savard; André Broes; Olivier Ariel; Gilles Meyer; Mariette F. Ducatez. Influenza D virus in respiratory disease in Canadian, province of Québec, cattle: Relative importance and evidence of new reassortment between different clades. Transboundary and Emerging Diseases 2021, 1 .
AMA StyleClaude Saegerman, Maria Gaudino, Christian Savard, André Broes, Olivier Ariel, Gilles Meyer, Mariette F. Ducatez. Influenza D virus in respiratory disease in Canadian, province of Québec, cattle: Relative importance and evidence of new reassortment between different clades. Transboundary and Emerging Diseases. 2021; ():1.
Chicago/Turabian StyleClaude Saegerman; Maria Gaudino; Christian Savard; André Broes; Olivier Ariel; Gilles Meyer; Mariette F. Ducatez. 2021. "Influenza D virus in respiratory disease in Canadian, province of Québec, cattle: Relative importance and evidence of new reassortment between different clades." Transboundary and Emerging Diseases , no. : 1.
Sub-Saharan Africa was historically considered an animal influenza cold spot, with only sporadic highly pathogenic H5 outbreaks detected over the last 20 years. However, in 2017, low pathogenic avian influenza A(H9N2) viruses were detected in poultry in Sub-Saharan Africa. Molecular, phylogenetic, and antigenic characterization of isolates from Benin, Togo, and Uganda showed that they belonged to the G1 lineage. Isolates from Benin and Togo clustered with viruses previously described in Western Africa, whereas viruses from Uganda were genetically distant and clustered with viruses from the Middle East. Viruses from Benin exhibited decreased cross-reactivity with those from Togo and Uganda, suggesting antigenic drift associated with reduced replication in Calu-3 cells. The viruses exhibited mammalian adaptation markers similar to those of the human strain A/Senegal/0243/2019 (H9N2). Therefore, viral genetic and antigenic surveillance in Africa is of paramount importance to detect further evolution or emergence of new zoonotic strains.
Maxime Fusade-Boyer; Fidélia Djegui; Komla Batawui; Denis K. Byuragaba; Jeremy C. Jones; Fred Wabwire-Mangeni; Bernard Erima; Gladys Atim; Qouilazoni A. Ukuli; Titus Tugume; Koffi Dogno; Komlan Adjabli; Mvibudulu Nzuzi; Rachidatou Adjin; Trushar Jeevan; Adam Rubrum; Wolali Go-Maro; Ghazi Kayali; Pamela McKenzie; Richard J. Webby; Mariette F. Ducatez. Antigenic and molecular characterization of low pathogenic avian influenza A(H9N2) viruses in sub-Saharan Africa from 2017 through 2019. Emerging Microbes & Infections 2021, 10, 753 -761.
AMA StyleMaxime Fusade-Boyer, Fidélia Djegui, Komla Batawui, Denis K. Byuragaba, Jeremy C. Jones, Fred Wabwire-Mangeni, Bernard Erima, Gladys Atim, Qouilazoni A. Ukuli, Titus Tugume, Koffi Dogno, Komlan Adjabli, Mvibudulu Nzuzi, Rachidatou Adjin, Trushar Jeevan, Adam Rubrum, Wolali Go-Maro, Ghazi Kayali, Pamela McKenzie, Richard J. Webby, Mariette F. Ducatez. Antigenic and molecular characterization of low pathogenic avian influenza A(H9N2) viruses in sub-Saharan Africa from 2017 through 2019. Emerging Microbes & Infections. 2021; 10 (1):753-761.
Chicago/Turabian StyleMaxime Fusade-Boyer; Fidélia Djegui; Komla Batawui; Denis K. Byuragaba; Jeremy C. Jones; Fred Wabwire-Mangeni; Bernard Erima; Gladys Atim; Qouilazoni A. Ukuli; Titus Tugume; Koffi Dogno; Komlan Adjabli; Mvibudulu Nzuzi; Rachidatou Adjin; Trushar Jeevan; Adam Rubrum; Wolali Go-Maro; Ghazi Kayali; Pamela McKenzie; Richard J. Webby; Mariette F. Ducatez. 2021. "Antigenic and molecular characterization of low pathogenic avian influenza A(H9N2) viruses in sub-Saharan Africa from 2017 through 2019." Emerging Microbes & Infections 10, no. 1: 753-761.
The influenza D virus (IDV) was first identified and characterized in 2011. Considering the virus’ zoonotic potential, its genome nature (segmented RNA virus), its worldwide circulation in livestock, and its role in bovine respiratory disease, an increased interest is given to IDV. However, few data are available on drivers of emergence of IDV. We first listed fifty possible drivers of emergence of IDV in ruminants and swine. As recently carried out for COVID19 in pets (Saegerman e,t al, 2020b), a scoring system was developed per driver and scientific experts (N = 28) were elicited to: (i) allocate a score to each driver, (ii) weight the drivers scores within each domain and (iii) weight the different domains among themselves. An overall weighted score was calculated per driver; and drivers were ranked in decreasing order. Drivers with comparable likelihoods to play a role in the emergence of IDV in ruminants and swine in Europe were grouped using a regression tree analysis. Finally, the robustness of the expert elicitation was verified. Eight drivers were ranked with the highest probability to play a key role in the emergence of IDV: current species specificity of the causing agent of the disease; influence of (il)legal movements of live animals (ruminants, swine) from neighbouring/European Union member states and from Third countries for the disease to (re‐)emerge in a given country; detection of emergence; current knowledge of the pathogen; vaccine availability; animal density; and transport vehicles of live animals. As there is still limited scientific knowledge on the topic, expert elicitation of knowledge and multi‐criteria decision analysis, in addition to clustering and sensitivity analyses, are very important to prioritize future studies, starting from the top eight drivers. The present methodology could be applied to other emerging animal diseases.
Claude Saegerman; Juana Bianchini; Chantal J. Snoeck; Ana Moreno; Chiara Chiapponi; Siamak Zohari; Mariette F. Ducatez. First expert elicitation of knowledge on drivers of emergence of influenza D in Europe. Transboundary and Emerging Diseases 2020, 1 .
AMA StyleClaude Saegerman, Juana Bianchini, Chantal J. Snoeck, Ana Moreno, Chiara Chiapponi, Siamak Zohari, Mariette F. Ducatez. First expert elicitation of knowledge on drivers of emergence of influenza D in Europe. Transboundary and Emerging Diseases. 2020; ():1.
Chicago/Turabian StyleClaude Saegerman; Juana Bianchini; Chantal J. Snoeck; Ana Moreno; Chiara Chiapponi; Siamak Zohari; Mariette F. Ducatez. 2020. "First expert elicitation of knowledge on drivers of emergence of influenza D in Europe." Transboundary and Emerging Diseases , no. : 1.
Maria Gaudino; Ana Moreno; Chantal J. Snoeck; Siamak Zohari; Claude Saegerman; Tom O’Donovan; Eoin Ryan; Irene Zanni; Emanuela Foni; Aurelie Sausy; Judith M. Hübschen; Gilles Meyer; Chiara Chiapponi; Mariette F. Ducatez. Emerging Influenza D virus infection in European livestock as determined in serology studies: Are we underestimating its spread over the continent? Transboundary and Emerging Diseases 2020, 68, 1125 -1135.
AMA StyleMaria Gaudino, Ana Moreno, Chantal J. Snoeck, Siamak Zohari, Claude Saegerman, Tom O’Donovan, Eoin Ryan, Irene Zanni, Emanuela Foni, Aurelie Sausy, Judith M. Hübschen, Gilles Meyer, Chiara Chiapponi, Mariette F. Ducatez. Emerging Influenza D virus infection in European livestock as determined in serology studies: Are we underestimating its spread over the continent? Transboundary and Emerging Diseases. 2020; 68 (3):1125-1135.
Chicago/Turabian StyleMaria Gaudino; Ana Moreno; Chantal J. Snoeck; Siamak Zohari; Claude Saegerman; Tom O’Donovan; Eoin Ryan; Irene Zanni; Emanuela Foni; Aurelie Sausy; Judith M. Hübschen; Gilles Meyer; Chiara Chiapponi; Mariette F. Ducatez. 2020. "Emerging Influenza D virus infection in European livestock as determined in serology studies: Are we underestimating its spread over the continent?" Transboundary and Emerging Diseases 68, no. 3: 1125-1135.
The influenza D virus (IDV) was discovered less than ten years ago. Increased interest in this virus is due to its nature (RNA virus with high mutation rate), its worldwide circulation in livestock species, its probable role in bovine respiratory disease and its zoonotic potential. Until currently, the establishment of positivity cut‐off of the hemagglutination inhibition (HI) assay was not formalized in field conditions for the detection of antibodies directed against IDV in cattle (i.e. the proposed reservoir). In this study, the positivity cut‐off of the HI assays was formally established (titre = 10) using a Receiver Operating Characteristic (ROC) curve. This information was used in order to estimate the sensitivity (68.04 to 73.20 %) and the specificity (94.17 to 96.12 %) of two different HI assays (HI1 and HI2, with two different IDV antigens) relatively to virus micro‐neutralisation test (VNT) as reference test. Based on the above characteristics, the true prevalence of IDV was then estimated in Morocco using a stochastic approach. Irrespective of the HI assays used, the estimation of the true prevalence was statistically equivalent (between 48.44 and 48.73 %). In addition, the spearman rank correlation between HI titres and VNT titres was statistically good (0.76 and 0.81 for HA1 and HA2, respectively). The positive (0.82 and 0.79 for HA1 and HA2, respectively) and the negative (0.86 and 0.85 for HA1 and HA2, respectively) agreement indices between results of HI assays and VNT were good and similar. This study allowed for a formal establishment of a positivity cut‐off in HI assays for the detection of antibodies directed against IDV. This information is of prime importance to estimate the diagnostic sensitivity and specificity of the test relatively to the VNT (i.e. the reference test). Using these characteristics, the true prevalence of IDV should be determined in a country.
Claude Saegerman; Elias Salem; Hicham Ait Lbacha; Said Alali; Zaid Zouagui; Gilles Meyer; Mariette F. Ducatez. Formal estimation of the seropositivity cut‐off of the hemagglutination inhibition assay in field diagnosis of influenza D virus in cattle and estimation of the associated true prevalence in Morocco. Transboundary and Emerging Diseases 2020, 68, 1392 -1399.
AMA StyleClaude Saegerman, Elias Salem, Hicham Ait Lbacha, Said Alali, Zaid Zouagui, Gilles Meyer, Mariette F. Ducatez. Formal estimation of the seropositivity cut‐off of the hemagglutination inhibition assay in field diagnosis of influenza D virus in cattle and estimation of the associated true prevalence in Morocco. Transboundary and Emerging Diseases. 2020; 68 (3):1392-1399.
Chicago/Turabian StyleClaude Saegerman; Elias Salem; Hicham Ait Lbacha; Said Alali; Zaid Zouagui; Gilles Meyer; Mariette F. Ducatez. 2020. "Formal estimation of the seropositivity cut‐off of the hemagglutination inhibition assay in field diagnosis of influenza D virus in cattle and estimation of the associated true prevalence in Morocco." Transboundary and Emerging Diseases 68, no. 3: 1392-1399.
H9N2 avian influenza viruses (AIV) has a worldwide geographic distribution and affects poultry of different types of production. H9N2 AIV was first reported in the Northeast of Algeria in April 2017, following an outbreak associated with high mortality, in broiler flocks. In the present study, we report full-length genome sequences of AIV H9N2, and the detailed phylogeny and molecular genetic analyses. Ten AIV H9N2 strains, collected in broiler flocks, were amplified in 9-day-old embryonated specific pathogen free (SPF) chicken eggs. Their full-length genomes were successfully sequenced and phylogenetic and molecular characterizations were conducted. Phylogenetic analysis showed that the isolates were monophyletic, grouped within the G-1 lineage and were very close to Moroccan and Algerian strains identified in 2016 and 2017, respectively. The low pathogenicity of the strains was confirmed by the sequence motif (335RSSR/GLF341) at the hemagglutinin (HA) cleavage site. An exclusive substitution (T197A) that had not been previously reported for H9N2 viruses; but, conserved in some pandemic H1N1 viruses, was observed. When compared to the G1-like H9N2 prototype, the studied strains showed one less glycosylation site in HA, but 2–3 additional ones in the stalk of the neuraminidase (NA). The HA protein harbored the substitution 234 L, suggesting binding preference to human-like receptors. The NA protein harbored S372A and R403W substitutions, previously detected in H9N2 from Asia and the Middle East, and especially in H2N2 and H3N2 strains that caused human pandemics. Different molecular markers associated with virulence and mammalian infections have been detected in the viral internal proteins. The matrix M2 protein possessed the S31N substitution associated with drug resistance. The non-structural 1 (NS1) protein showed the “GSEV” PDZ ligand (PL) C-terminal motif and no 80–84 deletion. Characterized Algerian AIV isolates showed mutations that suggest increased zoonotic potential. Additional studies in animal models are required to investigate the pathogenicity of these H9N2 AIV strains. Monitoring their evolution in both migratory and domestic birds is crucial to prevent transmission to humans. Implementation of adequate biosecurity measures that limit the introduction and the propagation of AIV H9N2 in Algerian poultry farm is crucial.
Abdelheq Barberis; Amine Boudaoud; Angelina Gorrill; Josianne Loupias; Abdeljelil Ghram; Jihene Lachheb; Nadir Alloui; Mariette F. Ducatez. Full-length genome sequences of the first H9N2 avian influenza viruses isolated in the Northeast of Algeria. Virology Journal 2020, 17, 1 -16.
AMA StyleAbdelheq Barberis, Amine Boudaoud, Angelina Gorrill, Josianne Loupias, Abdeljelil Ghram, Jihene Lachheb, Nadir Alloui, Mariette F. Ducatez. Full-length genome sequences of the first H9N2 avian influenza viruses isolated in the Northeast of Algeria. Virology Journal. 2020; 17 (1):1-16.
Chicago/Turabian StyleAbdelheq Barberis; Amine Boudaoud; Angelina Gorrill; Josianne Loupias; Abdeljelil Ghram; Jihene Lachheb; Nadir Alloui; Mariette F. Ducatez. 2020. "Full-length genome sequences of the first H9N2 avian influenza viruses isolated in the Northeast of Algeria." Virology Journal 17, no. 1: 1-16.
CD5 is characterized as an inhibitory coreceptor with an important regulatory role during T cell development. The molecular mechanism by which CD5 operates has been puzzling and its function in mature T cells suggests promoting rather than repressing effects on immune responses. Here, we combined quantitative mass spectrometry and genetic studies to analyze the components and the activity of the CD5 signaling machinery in primary T cells. We found that T cell receptor (TCR) engagement induces the selective phosphorylation of CD5 tyrosine 429, which serves as a docking site for proteins with adaptor functions (c-Cbl, CIN85, CRKL), connecting CD5 to positive (PI3K) and negative (UBASH3A, SHIP1) regulators of TCR signaling. c-CBL acts as a coordinator in this complex enabling CD5 to synchronize positive and negative feedbacks on TCR signaling through the other components. Disruption of CD5 signalosome in mutant mice reveals that it modulates TCR signal outputs to selectively repress the transactivation ofFoxp3and limit the inopportune induction of peripherally induced regulatory T cells during immune responses against foreign antigen. Our findings bring insights into the paradigm of coreceptor signaling, suggesting that, in addition to providing dualistic enhancing or dampening inputs, coreceptors can engage concomitant stimulatory and inhibitory signaling events, which act together to promote specific functional outcomes.
Gaëtan Blaize; Hélène Daniels-Treffandier; Meryem Aloulou; Nelly Rouquié; Cui Yang; Marlène Marcellin; Mylène Gador; Mehdi Benamar; Mariette Ducatez; Ki-Duk Song; Odile Burlet-Schiltz; Abdelhadi Saoudi; Paul E. Love; Nicolas Fazilleau; Anne Gonzalez de Peredo; Renaud Lesourne. CD5 signalosome coordinates antagonist TCR signals to control the generation of Treg cells induced by foreign antigens. Proceedings of the National Academy of Sciences 2020, 117, 12969 -12979.
AMA StyleGaëtan Blaize, Hélène Daniels-Treffandier, Meryem Aloulou, Nelly Rouquié, Cui Yang, Marlène Marcellin, Mylène Gador, Mehdi Benamar, Mariette Ducatez, Ki-Duk Song, Odile Burlet-Schiltz, Abdelhadi Saoudi, Paul E. Love, Nicolas Fazilleau, Anne Gonzalez de Peredo, Renaud Lesourne. CD5 signalosome coordinates antagonist TCR signals to control the generation of Treg cells induced by foreign antigens. Proceedings of the National Academy of Sciences. 2020; 117 (23):12969-12979.
Chicago/Turabian StyleGaëtan Blaize; Hélène Daniels-Treffandier; Meryem Aloulou; Nelly Rouquié; Cui Yang; Marlène Marcellin; Mylène Gador; Mehdi Benamar; Mariette Ducatez; Ki-Duk Song; Odile Burlet-Schiltz; Abdelhadi Saoudi; Paul E. Love; Nicolas Fazilleau; Anne Gonzalez de Peredo; Renaud Lesourne. 2020. "CD5 signalosome coordinates antagonist TCR signals to control the generation of Treg cells induced by foreign antigens." Proceedings of the National Academy of Sciences 117, no. 23: 12969-12979.
Bovine coronavirus (BCoV) is widespread in cattle and wild ruminant populations throughout the world. The virus causes neonatal calf diarrhea and winter dysentery in adult cattle, as well as upper and lower respiratory tract infection in young cattle. We isolated and deep sequenced whole genomes of BCoV from calves with respiratory distress in the south–west of France and conducted a comparative genome analysis using globally collected BCoV sequences to provide insights into the genomic characteristics, evolutionary origins, and global diversity of BCoV. Molecular clock analyses allowed us to estimate that the BCoV ancestor emerged in the 1940s, and that two geographically distinct lineages diverged from the 1960s–1970s. A recombination event in the spike gene (breakpoint at nt 1100) may be at the origin of the genetic divergence sixty years ago. Little evidence of genetic mixing between the spatially segregated lineages was found, suggesting that BCoV genetic diversity is a result of a global transmission pathway that occurred during the last century. However, we found variation in evolution rates between the European and non-European lineages indicating differences in virus ecology.
Elias Salem; Vijaykrishna Dhanasekaran; Herve Cassard; Ben Hause; Sarah Maman; Gilles Meyer; Mariette F. Ducatez. Global Transmission, Spatial Segregation, and Recombination Determine the Long-Term Evolution and Epidemiology of Bovine Coronaviruses. Viruses 2020, 12, 534 .
AMA StyleElias Salem, Vijaykrishna Dhanasekaran, Herve Cassard, Ben Hause, Sarah Maman, Gilles Meyer, Mariette F. Ducatez. Global Transmission, Spatial Segregation, and Recombination Determine the Long-Term Evolution and Epidemiology of Bovine Coronaviruses. Viruses. 2020; 12 (5):534.
Chicago/Turabian StyleElias Salem; Vijaykrishna Dhanasekaran; Herve Cassard; Ben Hause; Sarah Maman; Gilles Meyer; Mariette F. Ducatez. 2020. "Global Transmission, Spatial Segregation, and Recombination Determine the Long-Term Evolution and Epidemiology of Bovine Coronaviruses." Viruses 12, no. 5: 534.
Influenza D virus (IDV), a new genus of Orthomyxoviridae family, presents a large host range and a worldwide circulation. The pathogenicity of this virus has been studied in the calf model. The mouse model is frequently used to enable a first assessment of a pathogen ’ s fitness, replication, and pathogenesis for influenza A and B viruses. We showed that DBA/2 mice are a relevant in vivo model for the study of IDV replication. This model will allow for rapid IDV fitness and replication evaluation and will enable phenotypic comparisons between isolated viruses. It will also allow for a better understanding of the immune response induced after IDV infection.
J. Oliva; J. Mettier; L. Sedano; M. Delverdier; N. Bourgès-Abella; B. Hause; J. Loupias; I. Pardo; C. Bleuart; P. J. Bordignon; E. Meunier; R. Le Goffic; G. Meyer; M. F. Ducatez. Murine Model for the Study of Influenza D Virus. Journal of Virology 2020, 94, 1 .
AMA StyleJ. Oliva, J. Mettier, L. Sedano, M. Delverdier, N. Bourgès-Abella, B. Hause, J. Loupias, I. Pardo, C. Bleuart, P. J. Bordignon, E. Meunier, R. Le Goffic, G. Meyer, M. F. Ducatez. Murine Model for the Study of Influenza D Virus. Journal of Virology. 2020; 94 (4):1.
Chicago/Turabian StyleJ. Oliva; J. Mettier; L. Sedano; M. Delverdier; N. Bourgès-Abella; B. Hause; J. Loupias; I. Pardo; C. Bleuart; P. J. Bordignon; E. Meunier; R. Le Goffic; G. Meyer; M. F. Ducatez. 2020. "Murine Model for the Study of Influenza D Virus." Journal of Virology 94, no. 4: 1.
Influenza D virus (IDV) has been identified in several continents, with serological evidence for the virus in Africa. In order to improve the sensitivity and cost–benefit of IDV surveillance in Togo, risk maps were drawn using a spatial multicriteria decision analysis (MCDA) and experts’ opinion to evaluate the relevance of sampling areas used so far. Areas at highest risk of IDV occurrence were the main cattle markets. The maps were evaluated with previous field surveillance data collected in Togo between 2017 and 2019: 1216 sera from cattle, small ruminants, and swine were screened for antibodies to IDV by hemagglutination inhibition (HI) assays. While further samples collections are needed to validate the maps, the risk maps resulting from the spatial MCDA approach generated here highlight several priority areas for IDV circulation assessment.
Maxime Fusade-Boyer; Pidemnéwé S. Pato; Mathias Komlan; Koffi Dogno; Komla Batawui; Emilie Go-Maro; Pamela McKenzie; Claire Guinat; Aurélie Secula; Mathilde Paul; Richard J. Webby; Annelise Tran; Agnès Waret-Szkuta; Mariette F. Ducatez. Risk Mapping of Influenza D Virus Occurrence in Ruminants and Swine in Togo Using a Spatial Multicriteria Decision Analysis Approach. Viruses 2020, 12, 128 .
AMA StyleMaxime Fusade-Boyer, Pidemnéwé S. Pato, Mathias Komlan, Koffi Dogno, Komla Batawui, Emilie Go-Maro, Pamela McKenzie, Claire Guinat, Aurélie Secula, Mathilde Paul, Richard J. Webby, Annelise Tran, Agnès Waret-Szkuta, Mariette F. Ducatez. Risk Mapping of Influenza D Virus Occurrence in Ruminants and Swine in Togo Using a Spatial Multicriteria Decision Analysis Approach. Viruses. 2020; 12 (2):128.
Chicago/Turabian StyleMaxime Fusade-Boyer; Pidemnéwé S. Pato; Mathias Komlan; Koffi Dogno; Komla Batawui; Emilie Go-Maro; Pamela McKenzie; Claire Guinat; Aurélie Secula; Mathilde Paul; Richard J. Webby; Annelise Tran; Agnès Waret-Szkuta; Mariette F. Ducatez. 2020. "Risk Mapping of Influenza D Virus Occurrence in Ruminants and Swine in Togo Using a Spatial Multicriteria Decision Analysis Approach." Viruses 12, no. 2: 128.
Emerging of very virulent infectious bursal disease virus (vvIBDV) genotype in poultry flocks in Morocco were characterized. VP2 sequence analysis showed that the strains of Moroccan vvIBDV genotypes clustered separately from classic and vaccine strains reference of IBDV. The full-length genome of four Moroccan vvIBDV strains was determined, in order to get a more exhaustive molecular characterization allowing to conduct the evolution time scale and speculations on their origin. In a phylogenetic tree, nucleotide sequences of segment A and B formed a common branch with those vvIBDV references strains published in GenBank, but they clearly grouped into a distinct subcluster. An alignment of deduced amino acid sequences segment B, confirmed the presence of the conserved TDN tripeptide found in all of the vvIBDV genotype and revealed the presence of 2 substitutions I472L and E688D specific for the vvIBDV Moroccan isolates. The deduced amino acid sequences of segment A genes showed the presence of the "signature" typical of the vvIBDV genotype and revealed the presence of 7 aa substitutions specific for the vvIBDV Moroccan strains. The evolution rate for IBDV VP2 gene was estimated at 5.875 × 10-4 substitutions/site/year. The estimation of the time to most common recent ancestor of Moroccan vvIBDV based on the VP2 sequences available was 31 years, corresponding to 3 years earlier than the first vvIBDV case detection in layers in the country.
Charifa Drissi Touzani; Siham Fellahi; Ouafaa Fassi Fihri; Fatima Gaboun; Slimane Khayi; Rachid Mentag; Chiara Lico; Selene Baschieri; Mohammed El Houadfi; Mariette Ducatez. Complete genome analysis and time scale evolution of very virulent infectious bursal disease viruses isolated from recent outbreaks in Morocco. Infection, Genetics and Evolution 2019, 77, 104097 .
AMA StyleCharifa Drissi Touzani, Siham Fellahi, Ouafaa Fassi Fihri, Fatima Gaboun, Slimane Khayi, Rachid Mentag, Chiara Lico, Selene Baschieri, Mohammed El Houadfi, Mariette Ducatez. Complete genome analysis and time scale evolution of very virulent infectious bursal disease viruses isolated from recent outbreaks in Morocco. Infection, Genetics and Evolution. 2019; 77 ():104097.
Chicago/Turabian StyleCharifa Drissi Touzani; Siham Fellahi; Ouafaa Fassi Fihri; Fatima Gaboun; Slimane Khayi; Rachid Mentag; Chiara Lico; Selene Baschieri; Mohammed El Houadfi; Mariette Ducatez. 2019. "Complete genome analysis and time scale evolution of very virulent infectious bursal disease viruses isolated from recent outbreaks in Morocco." Infection, Genetics and Evolution 77, no. : 104097.
Background Viral diseases are a matter of great concern for poultry farmers in Pakistan. Multiple common viral respiratory diseases (CVRDs) cause huge economic losses in the poultry industry. The prevalence of CVRDs in many countries, including Pakistan, is not clearly understood. Results Incidences of 5 chicken respiratory viruses: avian influenza virus (AIV), Newcastle disease virus (NDV/AAVV-1), infectious bronchitis virus (IBV), avian metapneumovirus (aMPV) and infectious laryngotracheitis virus (ILTV) were assessed on commercial Pakistani farms with respiratory problems from 2014 through to 2016. While AIV and AAVV-1 were frequently detected (16 to 17% of farms), IBV and aMPV were rarely detected (in 3 to 5% of farms) and ILTV was not detected. We characterized H9 AIV of the G1 lineage, genotype VII AAVV-1, GI-13 IBV, and type B aMPV strains with very little genetic variability in the 2-year study period. Co-infections with AIV and AAVV-1 were common and wild type AAVV-1 was detected despite the use of vaccines. Control measures to limit the virus burden in chicken flocks are discussed. Conclusions Our data shows that AIV (H9), AAVV-1, IBV and aMPV are prevalent in commercial poultry in Pakistan. Further studies are necessary to assess circulating strains, economic losses caused by infections and coinfections of these pathogens, and the costs and benefits of countermeasures. Furthermore, veterinarians and farmers should be informed of the pathogens circulating in the field and hence advised on the use of vaccines.
Sajid Umar; Angélique Teillaud; Hassan Bin Aslam; Jean-Luc Guerin; Mariette F. Ducatez. Molecular epidemiology of respiratory viruses in commercial chicken flocks in Pakistan from 2014 through to 2016. BMC Veterinary Research 2019, 15, 351 .
AMA StyleSajid Umar, Angélique Teillaud, Hassan Bin Aslam, Jean-Luc Guerin, Mariette F. Ducatez. Molecular epidemiology of respiratory viruses in commercial chicken flocks in Pakistan from 2014 through to 2016. BMC Veterinary Research. 2019; 15 (1):351.
Chicago/Turabian StyleSajid Umar; Angélique Teillaud; Hassan Bin Aslam; Jean-Luc Guerin; Mariette F. Ducatez. 2019. "Molecular epidemiology of respiratory viruses in commercial chicken flocks in Pakistan from 2014 through to 2016." BMC Veterinary Research 15, no. 1: 351.
Since the emergence of low pathogenic avian influenza (LPAI) H9N2 viruses in Morocco in 2016, severe respiratory problems have been encountered in the field. Infectious bronchitis virus (IBV) is often detected together with H9N2, suggesting disease exacerbation in cases of co-infections. This hypothesis was therefore tested and confirmed in laboratory conditions using specific-pathogen-free chickens. Most common field vaccine programmes were then tested to compare their efficacies against these two co-infecting agents. IBV γCoV/chicken/Morocco/I38/2014 (Mor-IT02) and LPAI virus A/chicken/Morocco/SF1/2016 (Mor-H9N2) were thus inoculated to commercial chickens. We showed that vaccination with two heterologous IBV vaccines (H120 at day one and 4/91 at day 14 of age) reduced the severity of clinical signs as well as macroscopic lesions after simultaneous experimental challenge. In addition, LPAI H9N2 vaccination was more efficient at day 7 than at day 1 in limiting disease post simultaneous challenge. RESEARCH HIGHLIGHTS Simultaneous challenge with IBV and AIV H9N2 induced higher pathogenicity in SPF birds than inoculation with IBV or AIV H9N2 alone. Recommended vaccination programme in commercial broilers to counter Mor-IT02 IBV and LPAIV H9N2 simultaneous infections: IB live vaccine H120 (d1), AIV H9N2 inactivated vaccine (d7), IB live vaccine 4-91 (d14).
Sakhia F. Z. Belkasmi; Siham Fellahi; Charifa Drissi Touzani; Fatima Zahra Faraji; Imane Maaroufi; Maxence Delverdier; Jean-Luc Guérin; Ouafaa Fassi Fihri; Mohammed El Houadfi; Mariette F. Ducatez. Co-infections of chickens with avian influenza virus H9N2 and Moroccan Italy 02 infectious bronchitis virus: effect on pathogenesis and protection conferred by different vaccination programmes. Avian Pathology 2019, 49, 21 -28.
AMA StyleSakhia F. Z. Belkasmi, Siham Fellahi, Charifa Drissi Touzani, Fatima Zahra Faraji, Imane Maaroufi, Maxence Delverdier, Jean-Luc Guérin, Ouafaa Fassi Fihri, Mohammed El Houadfi, Mariette F. Ducatez. Co-infections of chickens with avian influenza virus H9N2 and Moroccan Italy 02 infectious bronchitis virus: effect on pathogenesis and protection conferred by different vaccination programmes. Avian Pathology. 2019; 49 (1):21-28.
Chicago/Turabian StyleSakhia F. Z. Belkasmi; Siham Fellahi; Charifa Drissi Touzani; Fatima Zahra Faraji; Imane Maaroufi; Maxence Delverdier; Jean-Luc Guérin; Ouafaa Fassi Fihri; Mohammed El Houadfi; Mariette F. Ducatez. 2019. "Co-infections of chickens with avian influenza virus H9N2 and Moroccan Italy 02 infectious bronchitis virus: effect on pathogenesis and protection conferred by different vaccination programmes." Avian Pathology 49, no. 1: 21-28.
Influenza D virus (IDV) has first been identified in 2011 in the USA and was shown to mainly circulate in cattle. While IDV is associated with mild respiratory signs, its prevalence is still unknown. In the present study we show that IDV has been circulating throughout France in cattle and small ruminants, with 47.2% and 1.5% seropositivity, respectively. The high prevalence and moderate pathogenicity of IDV in cattle suggest that it may play an initiating role in the bovine respiratory disease complex.
Justine Oliva; Amit Eichenbaum; Jade Belin; Maria Gaudino; Jean Guillotin; Jean-Pierre Alzieu; Philippe Nicollet; Roland Brugidou; Eric Gueneau; Evelyne Michel; Gilles Meyer; Mariette F. Ducatez. Serological Evidence of Influenza D Virus Circulation Among Cattle and Small Ruminants in France. Viruses 2019, 11, 516 .
AMA StyleJustine Oliva, Amit Eichenbaum, Jade Belin, Maria Gaudino, Jean Guillotin, Jean-Pierre Alzieu, Philippe Nicollet, Roland Brugidou, Eric Gueneau, Evelyne Michel, Gilles Meyer, Mariette F. Ducatez. Serological Evidence of Influenza D Virus Circulation Among Cattle and Small Ruminants in France. Viruses. 2019; 11 (6):516.
Chicago/Turabian StyleJustine Oliva; Amit Eichenbaum; Jade Belin; Maria Gaudino; Jean Guillotin; Jean-Pierre Alzieu; Philippe Nicollet; Roland Brugidou; Eric Gueneau; Evelyne Michel; Gilles Meyer; Mariette F. Ducatez. 2019. "Serological Evidence of Influenza D Virus Circulation Among Cattle and Small Ruminants in France." Viruses 11, no. 6: 516.
Avian coronaviruses cause major global problems in the poultry industry. As causative agents of huge economic losses, the detection and understanding of the molecular determinants of viral tropism are of ultimate importance. Here, we set out to study those parameters and obtained in-depth insight into the virus-host interactions of guinea fowl coronavirus (GfCoV). Our data indicate that diversity in GfCoV viral attachment proteins results in differences in degrees of affinity for glycan receptors, as well as altered avidity for intestinal tract tissues, which might have consequences for GfCoV tissue tropism and pathogenesis in guinea fowls.
Kim M. Bouwman; Mattias Delpont; Frederik Broszeit; Renaud Berger; Erik A. W. S. Weerts; Marie-Noëlle Lucas; Maxence Delverdier; Sakhia Belkasmi; Andreas Papanikolaou; Geert-Jan Boons; Jean-Luc Guérin; Robert de Vries; Mariette F. Ducatez; Monique H. Verheije. Guinea Fowl Coronavirus Diversity Has Phenotypic Consequences for Glycan and Tissue Binding. Journal of Virology 2019, 93, e00067-19 .
AMA StyleKim M. Bouwman, Mattias Delpont, Frederik Broszeit, Renaud Berger, Erik A. W. S. Weerts, Marie-Noëlle Lucas, Maxence Delverdier, Sakhia Belkasmi, Andreas Papanikolaou, Geert-Jan Boons, Jean-Luc Guérin, Robert de Vries, Mariette F. Ducatez, Monique H. Verheije. Guinea Fowl Coronavirus Diversity Has Phenotypic Consequences for Glycan and Tissue Binding. Journal of Virology. 2019; 93 (10):e00067-19.
Chicago/Turabian StyleKim M. Bouwman; Mattias Delpont; Frederik Broszeit; Renaud Berger; Erik A. W. S. Weerts; Marie-Noëlle Lucas; Maxence Delverdier; Sakhia Belkasmi; Andreas Papanikolaou; Geert-Jan Boons; Jean-Luc Guérin; Robert de Vries; Mariette F. Ducatez; Monique H. Verheije. 2019. "Guinea Fowl Coronavirus Diversity Has Phenotypic Consequences for Glycan and Tissue Binding." Journal of Virology 93, no. 10: e00067-19.
Respiratory syndromes (RS) are among the most significant pathological conditions in edible birds and are caused by complex coactions of pathogens and environmental factors. In poultry, low pathogenic avian influenza A viruses, metapneumoviruses, infectious bronchitis virus, infectious laryngotracheitis virus, Mycoplasma spp. Escherichia coli and/or Ornithobacterium rhinotracheale in turkeys are considered as key co-infectious agents of RS. Aspergillus sp., Pasteurella multocida, Avibacterium paragallinarum or Chlamydia psittaci may also be involved in respiratory outbreaks. An innovative quantitative PCR method, based on a nanofluidic technology, has the ability to screen up to 96 samples with 96 pathogen-specific PCR primers, at the same time, in one run of real-time quantitative PCR. This platform was used for the screening of avian respiratory pathogens: 15 respiratory agents, including viruses, bacteria and fungi potentially associated with respiratory infections of poultry, were targeted. Primers were designed and validated for SYBR green real-time quantitative PCR and subsequently validated on the Biomark high throughput PCR nanofluidic platform (Fluidigm©, San Francisco, CA, USA). As a clinical assessment, tracheal swabs were sampled from turkeys showing RS and submitted to this panel assay. Beside systematic detection of E. coli, avian metapneumovirus, Mycoplasma gallisepticum and Mycoplasma synoviae were frequently detected, with distinctive co-infection patterns between French and Moroccan flocks. This proof-of-concept study illustrates the potential of such panel assays for unveiling respiratory co-infection profiles in poultry.
Guillaume Croville; Charlotte Foret; Pauline Heuillard; Alexis Senet; Mattias Delpont; Mohammed Mouahid; Mariette F. Ducatez; Faouzi Kichou; Jean-Luc Guerin. Disclosing respiratory co-infections: a broad-range panel assay for avian respiratory pathogens on a nanofluidic PCR platform. Avian Pathology 2018, 47, 253 -260.
AMA StyleGuillaume Croville, Charlotte Foret, Pauline Heuillard, Alexis Senet, Mattias Delpont, Mohammed Mouahid, Mariette F. Ducatez, Faouzi Kichou, Jean-Luc Guerin. Disclosing respiratory co-infections: a broad-range panel assay for avian respiratory pathogens on a nanofluidic PCR platform. Avian Pathology. 2018; 47 (3):253-260.
Chicago/Turabian StyleGuillaume Croville; Charlotte Foret; Pauline Heuillard; Alexis Senet; Mattias Delpont; Mohammed Mouahid; Mariette F. Ducatez; Faouzi Kichou; Jean-Luc Guerin. 2018. "Disclosing respiratory co-infections: a broad-range panel assay for avian respiratory pathogens on a nanofluidic PCR platform." Avian Pathology 47, no. 3: 253-260.
Avian paramyxovirus type-1 (APMV-1) viruses of the lentogenic pathotypes are often isolated from wild aquatic birds and may mutate to high pathogenicity when they cross into poultry and cause debilitating Newcastle disease. This study characterised AMPV-1 isolated from fresh faecal droppings from wild aquatic birds roosting sites in Uganda. Fresh faecal samples from wild aquatic birds at several waterbodies in Uganda were collected and inoculated into 9–10-day-old embryonated chicken eggs. After isolation, the viruses were confirmed as APMV-1 by APMV-1-specific polymerase chain reaction (PCR). The cleavage site of the fusion protein gene for 24 representative isolates was sequenced and phylogenetically analysed and compared with representative isolates of the different APMV-1 genotypes in the GenBank database. In total, 711 samples were collected from different regions in the country from which 72 isolates were recovered, giving a prevalence of 10.1%. Sequence analysis of 24 isolates revealed that the isolates were all lentogenic, with the typical 111GGRQGR’L117 avirulent motif. Twenty-two isolates had similar amino acid sequences at the cleavage site, which were different from the LaSota vaccine strain by a silent nucleotide substitution T357C. Two isolates, NDV/waterfowl/Uganda/MU150/2011 and NDV/waterfowl/Uganda/MU186/2011, were different from the rest of the isolates in a single amino acid, with aspartate and alanine at positions 124 and 129, respectively. The results of this study revealed that Ugandan aquatic birds indeed harbour APMV-1 that clustered with class II genotype II strains and had limited genetic diversity.
Agnes Wanyana; Kizito K. Mugimba; Omony J. Bosco; Halid Kirunda; Jessica L. Nakavuma; Angélique Teillaud; Mariette F. Ducatez; Denis K. Byarugaba. Genotypic characterisation of Avian paramyxovirus type-1 viruses isolated from aquatic birds in Uganda. Onderstepoort Journal of Veterinary Research 2018, 85, 7 .
AMA StyleAgnes Wanyana, Kizito K. Mugimba, Omony J. Bosco, Halid Kirunda, Jessica L. Nakavuma, Angélique Teillaud, Mariette F. Ducatez, Denis K. Byarugaba. Genotypic characterisation of Avian paramyxovirus type-1 viruses isolated from aquatic birds in Uganda. Onderstepoort Journal of Veterinary Research. 2018; 85 (1):7.
Chicago/Turabian StyleAgnes Wanyana; Kizito K. Mugimba; Omony J. Bosco; Halid Kirunda; Jessica L. Nakavuma; Angélique Teillaud; Mariette F. Ducatez; Denis K. Byarugaba. 2018. "Genotypic characterisation of Avian paramyxovirus type-1 viruses isolated from aquatic birds in Uganda." Onderstepoort Journal of Veterinary Research 85, no. 1: 7.
Sakhia F. Z. Belkasmi; Siham Fellahi; Sajid Umar; Mattias Delpont; Maxence Delverdier; Marie-Noëlle Lucas; Céline Bleuart; Faouzi Kichou; Saadia Nassik; Jean-Luc Guerin; Ouafaa Fassi Fihri; Mariette F. Ducatez; Mohammed El Houadfi. Efficacy of Massachusetts and 793B Vaccines Against Infectious Bronchitis Moroccan-Italy 02 Virus in Specific-Pathogen-Free Chickens and Commercial Broilers. Avian Diseases 2017, 61, 466 -471.
AMA StyleSakhia F. Z. Belkasmi, Siham Fellahi, Sajid Umar, Mattias Delpont, Maxence Delverdier, Marie-Noëlle Lucas, Céline Bleuart, Faouzi Kichou, Saadia Nassik, Jean-Luc Guerin, Ouafaa Fassi Fihri, Mariette F. Ducatez, Mohammed El Houadfi. Efficacy of Massachusetts and 793B Vaccines Against Infectious Bronchitis Moroccan-Italy 02 Virus in Specific-Pathogen-Free Chickens and Commercial Broilers. Avian Diseases. 2017; 61 (4):466-471.
Chicago/Turabian StyleSakhia F. Z. Belkasmi; Siham Fellahi; Sajid Umar; Mattias Delpont; Maxence Delverdier; Marie-Noëlle Lucas; Céline Bleuart; Faouzi Kichou; Saadia Nassik; Jean-Luc Guerin; Ouafaa Fassi Fihri; Mariette F. Ducatez; Mohammed El Houadfi. 2017. "Efficacy of Massachusetts and 793B Vaccines Against Infectious Bronchitis Moroccan-Italy 02 Virus in Specific-Pathogen-Free Chickens and Commercial Broilers." Avian Diseases 61, no. 4: 466-471.