Forest ecosystems store approximately 45% of the carbon found in terrestrial ecosystems, but they are sensitive to climate-induced dieback. Forest die-off constitutes a large uncertainty in projections of climate impacts on terrestrial ecosystems, climate–ecosystem interactions, and carbon-cycle feedbacks. Current understanding of the physiological mechanisms mediating climate-induced forest mortality limits the ability to model or project these threshold events. We report here a direct and in situ study of the mechanisms underlying recent widespread and climate-induced trembling aspen ( Populus tremuloides ) forest mortality in western North America. We find substantial evidence of hydraulic failure of roots and branches linked to landscape patterns of canopy and root mortality in this species. On the contrary, we find no evidence that drought stress led to depletion of carbohydrate reserves. Our results illuminate proximate mechanisms underpinning recent aspen forest mortality and provide guidance for understanding and projecting forest die-offs under climate change.

Significance: Human-caused climate change could impact respiratory health, including asthma and allergies, through temperature-driven increases in airborne pollen, but the long-term continental pollen trends and role of climate change in pollen patterns are not well-understood. We measure pollen trends across North America from 1990 to 2018 and find increases in pollen concentrations and longer pollen seasons. We use an ensemble of climate models to test the role of climate change and find that it is the dominant driver of changes in pollen season length and a significant contributor to increasing pollen concentrations. Our results indicate that human-caused climate change has already worsened North American pollen seasons, and climate-driven pollen trends are likely to further exacerbate respiratory health impacts in coming decades.