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The ongoing global climate change and the associated environmental degradation pose a threat to Europe and the rest of the world. Raw materials and energy are required to produce building materials, which are used for construction purposes. Resulting buildings and structures generate waste during construction, operation, and demolition, and they emit potentially harmful substances. Thus, the key to achieving climate goals is to support low-emission materials and technologies in the construction sector, significantly impacting the environment. In the European Union, building materials are not yet subject to mandatory sustainability assessment during the assessment and verification of constancy of performance (AVCP). Objective evaluation of construction materials’ environmental impact requires it to be carried out based on production data on an industrial scale. This article presents the environmental impact of premixed gypsum-based plasters, commonly used in modern construction. Nine environmental indicators (global warming potential (GWP), depletion potential of the stratospheric ozone layer (ODP), acidification potential (AP), eutrophication potential (EP), formation potential of tropospheric ozone (POCP), abiotic depletion potential (ADP)-elements, ADP-fossil fuels, renewable primary energy resources (PERT), and nonrenewable primary energy resources (PERNT)) of premixed gypsum plasters based on natural and flue gas desulfurization (FGD) gypsum were estimated and discussed. Knowledge of the construction products’ environmental impact is fundamental for creating reliable databases. AVCP of construction materials in the future will use the data collected during the voluntary environmental impact evaluation.
Edyta Baran; Sebastian Czernik; Mariusz Hynowski; Bartosz Michałowski; Michał Piasecki; Justyna Tomaszewska; Jacek Michalak. Quantifying Environmental Burdens of Plasters Based on Natural vs. Flue Gas Desulfurization (FGD) Gypsum. Sustainability 2021, 13, 4298 .
AMA StyleEdyta Baran, Sebastian Czernik, Mariusz Hynowski, Bartosz Michałowski, Michał Piasecki, Justyna Tomaszewska, Jacek Michalak. Quantifying Environmental Burdens of Plasters Based on Natural vs. Flue Gas Desulfurization (FGD) Gypsum. Sustainability. 2021; 13 (8):4298.
Chicago/Turabian StyleEdyta Baran; Sebastian Czernik; Mariusz Hynowski; Bartosz Michałowski; Michał Piasecki; Justyna Tomaszewska; Jacek Michalak. 2021. "Quantifying Environmental Burdens of Plasters Based on Natural vs. Flue Gas Desulfurization (FGD) Gypsum." Sustainability 13, no. 8: 4298.
The building sector is one of the most critical branches of the economy influencing global warming today. Following the European Union’s Construction Products Regulation (CPR), the sustainable use of natural resources in construction is one of the seven basic buildings’ requirements. However, due to the lack of provisions in this regard, construction products are not yet subject to mandatory assessment and verification of the constancy of performance in this area. The paper presents the research results with the participation of 842 respondents who are investors, architects, contractors, and sellers of building materials. The analysis of their perceptions of issues related to the term sustainable development and knowledge of selected documents functioning in construction is presented. The share of people who correctly assigned the term sustainable development to equal treatment of environmental protection, economic development, and social development ranged between 52.5% and 65.7%. Knowledge of people associated with construction about the role of the declaration of performance (DoP) of construction products is high (from 73.9% to 99.4% correct answers). For Environmental Product Declaration (EPD), lower knowledge of this document’s role was noted, from 24.1% to 56.5% of correct answers. The obtained results indicate the need for further education.
Jacek Michalak; Bartosz Michałowski. Understanding of Construction Product Assessment Issues and Sustainability among Investors, Architects, Contractors, and Sellers of Construction Products in Poland. Energies 2021, 14, 1941 .
AMA StyleJacek Michalak, Bartosz Michałowski. Understanding of Construction Product Assessment Issues and Sustainability among Investors, Architects, Contractors, and Sellers of Construction Products in Poland. Energies. 2021; 14 (7):1941.
Chicago/Turabian StyleJacek Michalak; Bartosz Michałowski. 2021. "Understanding of Construction Product Assessment Issues and Sustainability among Investors, Architects, Contractors, and Sellers of Construction Products in Poland." Energies 14, no. 7: 1941.
External Thermal Insulation Composite System (ETICS) technology is commonly used to insulate buildings to increase the energy efficiency of external building envelopes. The ETICS is a construction product whose use has a significant impact on the environment and which itself is subject to an environmental impact assessment. The paper presents the impact of producing all ETICS components on the natural environment using the life cycle assessment method. The data used in the calculations, related to 2012 and 2017 real production, were obtained from the externally verified inventory from five manufacturing plants located in different regions of Poland. Life Cycle Assessment (LCA) of the examined products covered modules from A1 to A3 (Cradle-to-Gate) according to EN 15804 standard. That includes raw materials extraction, processing of secondary material, transport to the manufacturer, and production up to the finished, packed product at the factory gate. In the period of 2012–2017, the negative impact on the natural environment for most environmental indicators for the entire ETICS (all of its components), has been significantly reduced. For the first time, changes of such indicators as GWP, ODP, AP, EP, POCP, ADP, ADP, PERT, PENRT over 5 years period for ETICS have been presented.
Bartosz Michałowski; Jacek Michalak. Sustainability-oriented assessment of external thermal insulation composite systems: A case study from Poland. Cogent Engineering 2021, 8, 1 .
AMA StyleBartosz Michałowski, Jacek Michalak. Sustainability-oriented assessment of external thermal insulation composite systems: A case study from Poland. Cogent Engineering. 2021; 8 (1):1.
Chicago/Turabian StyleBartosz Michałowski; Jacek Michalak. 2021. "Sustainability-oriented assessment of external thermal insulation composite systems: A case study from Poland." Cogent Engineering 8, no. 1: 1.
Energy saving is one of the strategic challenges facing our civilization today. Without decisive actions to reduce energy consumption, it is impossible to maintain the current standard of living. Energy consumption for heating and cooling purposes is one of the primary energy consumption sources in many countries. The external thermal insulation composite system (ETICS), which is today the most widely used solution in EU countries, increases buildings’ energy efficiency. This article investigates the impact of producing cementitious adhesives, as part of ETICS with expanded polystyrene (EPS) or mineral wool (MW), on the natural environment using the cradle-to-gate life cycle assessment (LCA) method. Cementitious adhesives have a relatively low impact on most of the environmental indicators analyzed in the paper concerning other ETICS components. The paper aims to raise awareness of the importance of the environmental impact related to the production of cementitious adhesives. Knowledge of the construction products’ environmental impact is fundamental for creating reliable databases, based on which, in the future, their environmental requirements will be determined. The environmental performance of building elements is essential for the correct determination of the buildings’ sustainability.
Sebastian Czernik; Marta Marcinek; Bartosz Michałowski; Michał Piasecki; Justyna Tomaszewska; Jacek Michalak. Environmental Footprint of Cementitious Adhesives—Components of ETICS. Sustainability 2020, 12, 8998 .
AMA StyleSebastian Czernik, Marta Marcinek, Bartosz Michałowski, Michał Piasecki, Justyna Tomaszewska, Jacek Michalak. Environmental Footprint of Cementitious Adhesives—Components of ETICS. Sustainability. 2020; 12 (21):8998.
Chicago/Turabian StyleSebastian Czernik; Marta Marcinek; Bartosz Michałowski; Michał Piasecki; Justyna Tomaszewska; Jacek Michalak. 2020. "Environmental Footprint of Cementitious Adhesives—Components of ETICS." Sustainability 12, no. 21: 8998.
Assessment and verification of constancy of performance (AVCP) is a complex process. Without it, the manufacturer cannot mark the product with the Conformitè Europëenne (CE) marking and place it on the EU market. The verification of the correctness of the AVCP is carried out by market surveillance. In Poland, supervision authorities regularly check manufacturers by collecting construction products that exist on the market for inspection. Among the dry-mix mortars, adhesives for ceramic tiles (CTA) constitute an essential group. The requirements for CTAs are specified in EN 12004. According to the standard, the basic characteristics of cementitious CTAs are tensile adhesion strength and open time. The adhesion measurements are performed after various laboratory conditions tests. The study analyzes the results obtained for 129 samples of cementitious CTAs, collected between the years 2016 and 2019 from the market, and tested in notified laboratories at the request of Polish construction supervision authorities. Many tested products did not meet the threshold values, which resulted in removing them from the market. The paper discusses the complexity of the tensile adhesion strength measurement. The obtained test results are considered in various dimensions, including using the simple acceptance rule method when evaluating the results, which does not consider the measurement uncertainty.
Mateusz Łukasik; Bartosz Michałowski; Jacek Michalak. Assessment of the Constancy of Performance of Cementitious Adhesives for Ceramic Tiles: Analysis of the Test Results Commissioned by Polish Market Surveillance Authorities. Applied Sciences 2020, 10, 6561 .
AMA StyleMateusz Łukasik, Bartosz Michałowski, Jacek Michalak. Assessment of the Constancy of Performance of Cementitious Adhesives for Ceramic Tiles: Analysis of the Test Results Commissioned by Polish Market Surveillance Authorities. Applied Sciences. 2020; 10 (18):6561.
Chicago/Turabian StyleMateusz Łukasik; Bartosz Michałowski; Jacek Michalak. 2020. "Assessment of the Constancy of Performance of Cementitious Adhesives for Ceramic Tiles: Analysis of the Test Results Commissioned by Polish Market Surveillance Authorities." Applied Sciences 10, no. 18: 6561.
The external thermal insulation composite system (ETICS) improves the energy efficiency of buildings, and nowadays, this method is the most popular to insulate buildings in many European Union (EU) countries. The article presents the impact of producing ETICS with expanded polystyrene (EPS) or mineral wool (MW) on the natural environment using the life cycle assessment (LCA) method. The data used in the calculations, related to 2017 real production, were obtained from the externally verified inventory from five manufacturing plants located in different regions of Poland. The LCA of the examined products covered modules from A1 to A3 (cradle-to-gate), according to EN 15804 standard. The study determines and analyses the values of basic indicators related to environmental impacts and environmental aspects of resource use. It comprises indicators calculated for 1 m2 ETICS for five thicknesses of the mentioned thermal insulation materials. Results show that for all environmental indicators, MW systems are characterized by a more negative environmental impact than the equivalent systems with EPS. The study aims to highlight knowledge about ETICS sustainability. The data presented in work are essential for assessment in terms of the sustainable development of ETICS. Such an evaluation is not just a need for the future but a necessity for the present.
Jacek Michalak; Sebastian Czernik; Marta Marcinek; Bartosz Michałowski. Environmental burdens of External Thermal Insulation Systems. Expanded Polystyrene vs. Mineral Wool: Case Study from Poland. Sustainability 2020, 12, 4532 .
AMA StyleJacek Michalak, Sebastian Czernik, Marta Marcinek, Bartosz Michałowski. Environmental burdens of External Thermal Insulation Systems. Expanded Polystyrene vs. Mineral Wool: Case Study from Poland. Sustainability. 2020; 12 (11):4532.
Chicago/Turabian StyleJacek Michalak; Sebastian Czernik; Marta Marcinek; Bartosz Michałowski. 2020. "Environmental burdens of External Thermal Insulation Systems. Expanded Polystyrene vs. Mineral Wool: Case Study from Poland." Sustainability 12, no. 11: 4532.
External thermal insulation systems (ETICS) are relevant facade applications of functional components allowing to reduce energy consumption in buildings to fulfill the provisions of nearly Zero-Energy Buildings (nZEBs). ETICS systems generally are made of adhesives, thermal insulation material, renders with mesh reinforcement, primers, and finish coats. Their main parameters are thermal characteristics and durability, both determined by the specific composition of the systems. Growing concerns on the environment and depletion of natural resources drive the need for the determination of the environmental characteristic of ETICS due to its growing market demand. This analysis focuses on a life cycle assessment (LCA) of a commonly used EPS based ETICS system with four different renderings, produced in several locations. The scope of this study concerns raw materials extraction, transport, production, and energy provision up to the finished, packed, and ready-for-sale product at the factory gate. The authors compared the environmental impact allocated to the 1 m2 of the produced system by taking into account the thickness of EPS and within different environmental impact categories. The results of the current impacts were compared to those obtained five years earlier, considering the technological and environmental progress of the production process.
Bartosz Michałowski; Marta Marcinek; Justyna Tomaszewska; Sebastian Czernik; Michał Piasecki; Robert Geryło; Jacek Michalak. Influence of Rendering Type on the Environmental Characteristics of Expanded Polystyrene-Based External Thermal Insulation Composite System. Buildings 2020, 10, 47 .
AMA StyleBartosz Michałowski, Marta Marcinek, Justyna Tomaszewska, Sebastian Czernik, Michał Piasecki, Robert Geryło, Jacek Michalak. Influence of Rendering Type on the Environmental Characteristics of Expanded Polystyrene-Based External Thermal Insulation Composite System. Buildings. 2020; 10 (3):47.
Chicago/Turabian StyleBartosz Michałowski; Marta Marcinek; Justyna Tomaszewska; Sebastian Czernik; Michał Piasecki; Robert Geryło; Jacek Michalak. 2020. "Influence of Rendering Type on the Environmental Characteristics of Expanded Polystyrene-Based External Thermal Insulation Composite System." Buildings 10, no. 3: 47.
In order to mitigate and prevent chemical attack and concrete corrosion one has to choose the right concrete to make it less permeable or isolate it from the corrosive environment by using a suitable coating. Here, we present the use of geopolymer mortar made of local waste materials and sodium silicate as a chemo-resistive coating for concrete. In this paper, the development of geopolymer mortar working parameters (e.g. setting time and adhesion to concrete) is described. Here, we investigated the resistance of concrete samples coated with geopolymer to 10% inorganic/organic acids and saturated solution of sodium chloride. During the soaking in corrosive environment, weight loss and compressive strength were measured. Geopolymer coating described in this work showed great resistance to organic and inorganic acids. Coating of concrete samples reduced their weight loss after exposure to organic acids from 15% to 2% with concomitant compressive strength loss from 49% to 9% of the initial value. The difference in chemical resistance for inorganic acids was even more visible. Coating of concrete samples reduced their weight loss after exposure to inorganic acids from 73% to 0.8% with adequate compressive strength loss from 96% to only 3.5% of the initial value.
Szymon Sikora; Ewa Gapys; Bartosz Michalowski; Tomasz Horbanowicz; Mariusz Hynowski. Geopolymer coating as a protection of concrete against chemical attack and corrosion. E3S Web of Conferences 2018, 49, 00101 .
AMA StyleSzymon Sikora, Ewa Gapys, Bartosz Michalowski, Tomasz Horbanowicz, Mariusz Hynowski. Geopolymer coating as a protection of concrete against chemical attack and corrosion. E3S Web of Conferences. 2018; 49 ():00101.
Chicago/Turabian StyleSzymon Sikora; Ewa Gapys; Bartosz Michalowski; Tomasz Horbanowicz; Mariusz Hynowski. 2018. "Geopolymer coating as a protection of concrete against chemical attack and corrosion." E3S Web of Conferences 49, no. : 00101.
Boronate probes have emerged recently as a versatile tool for the detection of reactive oxygen and nitrogen species. Here we present characterization of fluorescein-based monoboronate probe, 4-(pinacol boronate)benzyl-derivative of fluorescein methyl ester (FBBE), that prove to be useful to detect peroxynitrite in cell culture experiments. Reactivity of FBBE towards peroxynitrite as well hypochlorite, hydrogen peroxide, and tyrosyl hydroperoxide was determined. Second-order rate constants of the reactions of FBBE with peroxynitrite , HOCl, and H2O2 at pH 7.4 were equal to (2.8 ± 0.2) × 105 M-1s-1, (8.6 ± 0.5) × 103 M-1s-1 and (0.96 ± 0.03) M−1s−1, respectively. The presence of glutathione completely blocked the oxidation of the probe by HOCl and significantly inhibited its oxidation by H2O2 and tyrosyl hydroperoxide, but not by peroxynitrite. The oxidative conversion of the probe was also studied in the systems generating singlet oxygen, superoxide radical anion and nitric oxide in the presence and absence of glutathione. Spectroscopic characterization of FBBE, and its oxidation product has been also performed. The differences in the reactivity pattern were supported by DFT quantum mechanical calculations. Finally, FBBE probe was used to study the oxidative stress in endothelial cells (Ea.hy926) incubated with doxorubicin, a quinone anthracycline antibiotic. In endothelial cells pretreated with doxorubicin, FBBE was oxidized and this effect was reversed by PEG-SOD and L-NAME, but not by catalase.
Karolina Dębowska; Dawid Dębski; Bartosz Michałowski; Agnieszka Dybala-Defratyka; Tomasz Wójcik; Radosław Michalski; Małgorzata Jakubowska; Anna Selmi; Renata Smulik; Łukasz Piotrowski; Jan Adamus; Andrzej Marcinek; Stefan Chlopicki; Adam Sikora. Characterization of Fluorescein-Based Monoboronate Probe and Its Application to the Detection of Peroxynitrite in Endothelial Cells Treated with Doxorubicin. Chemical Research in Toxicology 2016, 29, 735 -746.
AMA StyleKarolina Dębowska, Dawid Dębski, Bartosz Michałowski, Agnieszka Dybala-Defratyka, Tomasz Wójcik, Radosław Michalski, Małgorzata Jakubowska, Anna Selmi, Renata Smulik, Łukasz Piotrowski, Jan Adamus, Andrzej Marcinek, Stefan Chlopicki, Adam Sikora. Characterization of Fluorescein-Based Monoboronate Probe and Its Application to the Detection of Peroxynitrite in Endothelial Cells Treated with Doxorubicin. Chemical Research in Toxicology. 2016; 29 (5):735-746.
Chicago/Turabian StyleKarolina Dębowska; Dawid Dębski; Bartosz Michałowski; Agnieszka Dybala-Defratyka; Tomasz Wójcik; Radosław Michalski; Małgorzata Jakubowska; Anna Selmi; Renata Smulik; Łukasz Piotrowski; Jan Adamus; Andrzej Marcinek; Stefan Chlopicki; Adam Sikora. 2016. "Characterization of Fluorescein-Based Monoboronate Probe and Its Application to the Detection of Peroxynitrite in Endothelial Cells Treated with Doxorubicin." Chemical Research in Toxicology 29, no. 5: 735-746.
Amplex® Red (10-acetyl-3,7-dihydroxyphenoxazine) is a fluorogenic probe widely used to detect and quantify hydrogen peroxide in biological systems. Detection of hydrogen peroxide is based on peroxidase-catalyzed oxidation of Amplex® Red to resorufin. In this study we investigated the mechanism of one-electron oxidation of Amplex® Red and we present the spectroscopic characterization of transient species formed upon the oxidation. Oxidation process has been studied by a pulse radiolysis technique with one-electron oxidants (N3•, CO3•–, •NO2 and GS•). The rate constants for the Amplex® Red oxidation by N3• (2k=2.1·109 M−1s−1, at pH=7.2) and CO3•– (2k=7.6·108 M−1s−1, at pH=10.3) were determined. Two intermediates formed during the conversion of Amplex® Red into resorufin have been characterized. Based on the results obtained, the mechanism of transformation of Amplex® Red into resorufin, involving disproportionation of the Amplex® Red-derived radical species, has been proposed. The results indicate that peroxynitrite-derived radicals, but not peroxynitrite itself, are capable to oxidize Amplex® Red to resorufin. We also demonstrate that horseradish peroxidase can catalyze oxidation of Amplex® Red not only by hydrogen peroxide, but also by peroxynitrite, which needs to be considered when employing the probe for hydrogen peroxide detection.
Dawid Dębski; Renata Smulik-Izydorczyk; Jacek Zielonka; Bartosz Michalowski; Małgorzata Jakubowska; Karolina Dębowska; Jan Adamus; Andrzej Marcinek; Balaraman Kalyanaraman; Adam Sikora. Mechanism of oxidative conversion of Amplex® Red to resorufin: Pulse radiolysis and enzymatic studies. Free Radical Biology and Medicine 2016, 95, 323 -332.
AMA StyleDawid Dębski, Renata Smulik-Izydorczyk, Jacek Zielonka, Bartosz Michalowski, Małgorzata Jakubowska, Karolina Dębowska, Jan Adamus, Andrzej Marcinek, Balaraman Kalyanaraman, Adam Sikora. Mechanism of oxidative conversion of Amplex® Red to resorufin: Pulse radiolysis and enzymatic studies. Free Radical Biology and Medicine. 2016; 95 ():323-332.
Chicago/Turabian StyleDawid Dębski; Renata Smulik-Izydorczyk; Jacek Zielonka; Bartosz Michalowski; Małgorzata Jakubowska; Karolina Dębowska; Jan Adamus; Andrzej Marcinek; Balaraman Kalyanaraman; Adam Sikora. 2016. "Mechanism of oxidative conversion of Amplex® Red to resorufin: Pulse radiolysis and enzymatic studies." Free Radical Biology and Medicine 95, no. : 323-332.
Over the last 40 years, there has been tremendous progress in understanding the biological reactions of reactive oxygen species (ROS) and reactive nitrogen species (RNS). It is widely accepted that the generation of ROS and RNS is involved in physiological and pathophysiological processes. To understand the role of ROS and RNS in a variety of pathologies, the specific detection of ROS and RNS is fundamental. Unfortunately, the intracellular detection and quantitation of ROS and RNS remains a challenge. In this short review, we have focused on the mechanistic and quantitative aspects of their detection with the use of selected fluorogenic probes. The challenges, limitations and perspectives of these methods are discussed.
Karolina Debowska; Dawid Debski; Micael Hardy; Malgorzata Jakubowska; Balaraman Kalyanaraman; Andrzej Marcinek; Radoslaw Michalski; Bartosz Michalowski; Olivier Ouari; Adam Sikora; Renata Smulik-Izydorczyk; Jacek Zielonka. Toward selective detection of reactive oxygen and nitrogen species with the use of fluorogenic probes – Limitations, progress, and perspectives. Pharmacological Reports 2015, 67, 756 -764.
AMA StyleKarolina Debowska, Dawid Debski, Micael Hardy, Malgorzata Jakubowska, Balaraman Kalyanaraman, Andrzej Marcinek, Radoslaw Michalski, Bartosz Michalowski, Olivier Ouari, Adam Sikora, Renata Smulik-Izydorczyk, Jacek Zielonka. Toward selective detection of reactive oxygen and nitrogen species with the use of fluorogenic probes – Limitations, progress, and perspectives. Pharmacological Reports. 2015; 67 (4):756-764.
Chicago/Turabian StyleKarolina Debowska; Dawid Debski; Micael Hardy; Malgorzata Jakubowska; Balaraman Kalyanaraman; Andrzej Marcinek; Radoslaw Michalski; Bartosz Michalowski; Olivier Ouari; Adam Sikora; Renata Smulik-Izydorczyk; Jacek Zielonka. 2015. "Toward selective detection of reactive oxygen and nitrogen species with the use of fluorogenic probes – Limitations, progress, and perspectives." Pharmacological Reports 67, no. 4: 756-764.
Nitroxyl (HNO), the protonated one-electron reduction product of nitric oxide (NO), remains an enigmatic reactive nitrogen species. Its chemical reactivity and biological activity are still not completely understood. HNO donors show biological effects different from NO donors. Although HNO reactivity with molecular oxygen is described in the literature, the product of this reaction has not yet been unambiguously identified. Here we report, that the decomposition of HNO donors under aerobic conditions in aqueous solutions at physiological pH leads to the formation of peroxynitrite (ONOO−) as a major intermediate. We have specifically detected and quantified ONOO− with the aid of boronate probes, e.g., coumarin-7-boronic acid (CBA), or 4-boronobenzyl derivative of fluorescein methyl ester (FBBE). In addition to the major phenolic products, peroxynitrite-specific minor products of oxidation of boronate probes were detected under these conditions. Using the competition kinetics method and a set of HNO scavengers, the value of the second-order rate constant of the HNO reaction with oxygen (k = 1.8×10^4 M-1s-1) was determined. The rate constant (k = 2×10^4 M-1s-1) was also determined using kinetic simulations. The kinetic parameters of the reactions of HNO with selected thiols, including cysteine, dithiothreitol, N-acetylcysteine, captopril, bovine and human serum albumins and hydrogen sulfide are reported. Biological and cardiovascular implications of nitroxyl reactions are discussed.
Renata Smulik-Izydorczyk; Dawid Dębski; Jacek Zielonka; Bartosz Michalowski; Jan Adamus; Andrzej Marcinek; Balaraman Kalyanaraman; Adam Sikora. Nitroxyl (HNO) Reacts with Molecular Oxygen and Forms Peroxynitrite at Physiological pH. Journal of Biological Chemistry 2014, 289, 35570 -35581.
AMA StyleRenata Smulik-Izydorczyk, Dawid Dębski, Jacek Zielonka, Bartosz Michalowski, Jan Adamus, Andrzej Marcinek, Balaraman Kalyanaraman, Adam Sikora. Nitroxyl (HNO) Reacts with Molecular Oxygen and Forms Peroxynitrite at Physiological pH. Journal of Biological Chemistry. 2014; 289 (51):35570-35581.
Chicago/Turabian StyleRenata Smulik-Izydorczyk; Dawid Dębski; Jacek Zielonka; Bartosz Michalowski; Jan Adamus; Andrzej Marcinek; Balaraman Kalyanaraman; Adam Sikora. 2014. "Nitroxyl (HNO) Reacts with Molecular Oxygen and Forms Peroxynitrite at Physiological pH." Journal of Biological Chemistry 289, no. 51: 35570-35581.
Coffee may exert a preventive effect on arterial thrombosis. Trigonelline is one of the most abundant compounds in coffee that undergoes pyrolysis upon roasting of coffee beans. The aim of the present study was to identify pyridinium compounds formed upon trigonelline pyrolysis and coffee roasting and to investigate the effect of three of them, i.e., 1-methylpyridine and 1,3- and 1,4-dimethylpyridine, on experimentally induced arterial thrombosis in rats. 1,3- and 1,4-dimethylpyridine but not 1-methylpyridine inhibited arterial thrombus formation. 1,3-Dimethylpyridine inhibited platelet aggregation and reduced fibrin formation in platelet-rich plasma, whereas 1,4-dimethylpyridine increased the plasma level of 6-keto-PGF1α. 1,4-Dimethylpyridine slightly increased rat tissue plasminogen activator plasma activity. In summary, we demonstrated that pyridinium compounds display mild antithrombotic properties due to stimulation by prostacyclin release (1,4-dimethylpyridine) and inhibition of platelet aggregation (1,3-dimethylpyridine). Those pyridinium compounds may, to some extent, be responsible for the beneficial effects of coffee drinking.
Bartlomiej Kalaska; Lukasz Piotrowski; Agnieszka Leszczynska; Bartosz Michalowski; Karol Kramkowski; Tomasz Kaminski; Jan Adamus; Andrzej Marcinek; Jerzy Gebicki; Andrzej Mogielnicki; Wlodzimierz Buczko. Antithrombotic Effects of Pyridinium Compounds Formed from Trigonelline upon Coffee Roasting. Journal of Agricultural and Food Chemistry 2014, 62, 2853 -2860.
AMA StyleBartlomiej Kalaska, Lukasz Piotrowski, Agnieszka Leszczynska, Bartosz Michalowski, Karol Kramkowski, Tomasz Kaminski, Jan Adamus, Andrzej Marcinek, Jerzy Gebicki, Andrzej Mogielnicki, Wlodzimierz Buczko. Antithrombotic Effects of Pyridinium Compounds Formed from Trigonelline upon Coffee Roasting. Journal of Agricultural and Food Chemistry. 2014; 62 (13):2853-2860.
Chicago/Turabian StyleBartlomiej Kalaska; Lukasz Piotrowski; Agnieszka Leszczynska; Bartosz Michalowski; Karol Kramkowski; Tomasz Kaminski; Jan Adamus; Andrzej Marcinek; Jerzy Gebicki; Andrzej Mogielnicki; Wlodzimierz Buczko. 2014. "Antithrombotic Effects of Pyridinium Compounds Formed from Trigonelline upon Coffee Roasting." Journal of Agricultural and Food Chemistry 62, no. 13: 2853-2860.
Recently, D.J. Hall et al. reported that ethidium (E+) is formed as a major product of hydroethidine (HE) or dihydroethidium reaction with superoxide (O2−) in intact animals with low tissue oxygen levels (J. Cereb. Blood Flow Metab. 32:23–32, 2012). The authors concluded that measurement of E+ is an indicator of O2− formation in intact brains of animals. This finding is in stark contrast to previous reports using in vitro systems showing that 2-hydroxyethidium, not ethidium, is formed from the reaction between O2− and HE. Published in vivo results support the in vitro findings. In this study, we performed additional experiments in which HE oxidation products were monitored under different fluxes of O2−. Results from these experiments further reaffirm our earlier findings (H. Zhao et al., Free Radic. Biol. Med. 34:1359, 2003). We conclude that whether in vitro or in vivo, E+ measured by HPLC or by fluorescence lifetime imaging is not a diagnostic marker product for O2− reaction with HE.
Radoslaw Michalski; Bartosz Michalowski; Adam Sikora; Jacek Zielonka; Balaraman Kalyanaraman. On the use of fluorescence lifetime imaging and dihydroethidium to detect superoxide in intact animals and ex vivo tissues: A reassessment. Free Radical Biology and Medicine 2013, 67, 278 -284.
AMA StyleRadoslaw Michalski, Bartosz Michalowski, Adam Sikora, Jacek Zielonka, Balaraman Kalyanaraman. On the use of fluorescence lifetime imaging and dihydroethidium to detect superoxide in intact animals and ex vivo tissues: A reassessment. Free Radical Biology and Medicine. 2013; 67 ():278-284.
Chicago/Turabian StyleRadoslaw Michalski; Bartosz Michalowski; Adam Sikora; Jacek Zielonka; Balaraman Kalyanaraman. 2013. "On the use of fluorescence lifetime imaging and dihydroethidium to detect superoxide in intact animals and ex vivo tissues: A reassessment." Free Radical Biology and Medicine 67, no. : 278-284.
Aromatic boronic acids react rapidly with peroxynitrite (ONOO(-)) to yield phenols as major products. This reaction was used to monitor ONOO(-) formation in cellular systems. Previously, we proposed that the reaction between ONOO(-) and arylboronates (PhB(OH)2) yields a phenolic product (major pathway) and a radical pair PhB(OH)2O(•-)···(•)NO2 (minor pathway). [Sikora, A. et al. (2011) Chem. Res. Toxicol. 24, 687-697]. In this study, we investigated the influence of a bulky triphenylphosphonium (TPP) group on the reaction between ONOO(-) and mitochondria-targeted arylboronate isomers (o-, m-, and p-MitoPhB(OH)2). Results from the electron paramagnetic resonance (EPR) spin-trapping experiments unequivocally showed the presence of a phenyl radical intermediate from meta and para isomers, and not from the ortho isomer. The yield of o-MitoPhNO2 formed from the reaction between o-MitoPhB(OH)2 and ONOO(-) was not diminished by phenyl radical scavengers, suggesting a rapid fragmentation of the o-MitoPhB(OH)2O(•-) radical anion with subsequent reaction of the resulting phenyl radical with (•)NO2 in the solvent cage. The DFT quantum mechanical calculations showed that the energy barrier for the dissociation of the o-MitoPhB(OH)2O(•-) radical anion is significantly lower than that of m-MitoPhB(OH)2O(•-) and p-MitoPhB(OH)2O(•-) radical anions. The nitrated product, o-MitoPhNO2, is not formed by the nitrogen dioxide radical generated by myeloperoxidase in the presence of the nitrite anion and hydrogen peroxide, indicating that this specific nitrated product may be used as a diagnostic marker product for ONOO(-). Incubation of o-MitoPhB(OH)2 with RAW 264.7 macrophages activated to produce ONOO(-) yielded the corresponding phenol o-MitoPhOH as well as the diagnostic nitrated product, o-MitoPhNO2. We conclude that the ortho isomer probe reported here is most suitable for specific detection of ONOO(-) in biological systems.
Adam Sikora; Jacek Zielonka; Jan Adamus; Dawid Debski; Agnieszka Dybala-Defratyka; Bartosz Michalowski; Joy Joseph; Richard Hartley; Michael Murphy; Balaraman Kalyanaraman. Reaction between Peroxynitrite and Triphenylphosphonium-Substituted Arylboronic Acid Isomers: Identification of Diagnostic Marker Products and Biological Implications. Chemical Research in Toxicology 2013, 26, 856 -867.
AMA StyleAdam Sikora, Jacek Zielonka, Jan Adamus, Dawid Debski, Agnieszka Dybala-Defratyka, Bartosz Michalowski, Joy Joseph, Richard Hartley, Michael Murphy, Balaraman Kalyanaraman. Reaction between Peroxynitrite and Triphenylphosphonium-Substituted Arylboronic Acid Isomers: Identification of Diagnostic Marker Products and Biological Implications. Chemical Research in Toxicology. 2013; 26 (6):856-867.
Chicago/Turabian StyleAdam Sikora; Jacek Zielonka; Jan Adamus; Dawid Debski; Agnieszka Dybala-Defratyka; Bartosz Michalowski; Joy Joseph; Richard Hartley; Michael Murphy; Balaraman Kalyanaraman. 2013. "Reaction between Peroxynitrite and Triphenylphosphonium-Substituted Arylboronic Acid Isomers: Identification of Diagnostic Marker Products and Biological Implications." Chemical Research in Toxicology 26, no. 6: 856-867.