This study aimed to investigate the wound-healing efficacy of Hypericum perforatum and pomegranate seed extract oil combined with curcumin as an anti inflammatory agent. A series of experiments were carried out to determine effective concentrations for H. perforatum oil, pomegranate seed extract oil, and curcumin. Ibuprofen was used as a positive control. The wound-healing effects of the applied compounds were tested according to the migration experiment model performed in HaCaT cells. A real-time cell analyzer (xCELLigence) was used to determine the cytotoxic/proliferative effects of H. perforatum, pomegranate seed oil, ibuprofen, and curcumin in HaCaT cells alone and their combined use at specified concentrations. After examining the noncytotoxic concentrations of H. perforatum oil, pomegranate seed oil, curcumin, and ibuprofen, migration experiments were performed to examine the wound healing properties. According to the results, the wound-healing efficacy of curcumin and H. perforatum combination was better than ibuprofen combinations. Also, according to the results, the wound-healing efficacy of curcumin and pomegranate seed oil combination was better than ibuprofen combinations. It was concluded that both oils had improved wound-healing properties in combination with curcumin or Ibuprofen.

In Alzheimer’s disease (AD), neurofibrillary tangles are composed of hyperphosphorylated tau protein, and tau hyperphosphorylation reduces microtubule binding. Many protein kinases are thought to be involved in tau hyperphosphorylation. Based on the fact that tau hyperphosphorylation can be prevented by inhibition of glycogen synthase kinase-3β (GSK-3β), which is one of the tau kinases, the effectiveness of potential GSK-3β inhibitors determined by virtual screening, molecular docking, and dynamics simulations studies on Alzheimer’s pathology has been examined and its role in neurodegeneration has been investigated by studies. Neomangiferin was determined as the most effective molecule according to the results of studies with potential compounds determined by virtual screening and molecular docking to be GSK-3β inhibitors in the in vitro Alzheimer’s model created by neuronal differentiation studies. Neomangiferin has been shown to have a protective role in induced neurodegeneration by the MTT method and Real Time Cell Analysis. It has been determined that Neomangiferin inhibits GSK-3β and reduces the level of phosphorylated tau. In summary, our findings suggested Neomangiferin can be a therapeutic candidate for AD treatment. Communicated by Ramaswamy H. Sarma

Objective: SARS-CoV-2 is a pandemic virus characterized by upper respiratory tract infection and can range from mild symptoms to severe complications. In this case, drug repurposing and computer-aided studies have become very important to find emergency solutions. In this study, drug-target interactions on three nonstructural protein structures of SARS-CoV-2 of 8820 drug candidates or drug molecules obtained from the DrugBank database were analyzed. Material and Method: Comprehensive virtual screening and molecular docking studies from 8820 drug molecules or candidates obtained from the DrugBank database were performed on the RNA binding protein, 2'-O-methyltransferase, and endoribonuclease of SARS-CoV-2; and potential drug candidates were determined for each target. Virtual screening studies have been done with High-Throughput Virtual Screening (HTVS), Standard Precision (SP), Extra Precision (XP), and Molecular Mechanics Generalized Born Surface Area (MM-GBSA). Also, information about the clinical findings, transmission, pathogenesis, and treatment of SARS-CoV-2 has been given. Result and Discussion: Drug-target interactions on three nonstructural protein structures of SARS-CoV-2 of 8820 drug candidates or drug molecules obtained from the DrugBank database were analyzed. Potential compound recommendations for each drug target were presented. Information was given about key amino acids where active sites of drug target proteins interact with ligands. This study is expected to be useful in target-based drug development studies on the proteins of SARS-CoV-2. Keywords: SARS-CoV-2, molecular docking, virtual screening, DrugBank

Artan mikrobiyal enfeksiyon hastalıklara ve bunların tedavisinde kullanılan ilaçlara karşı hızla direnç gelişmesi nedeniyle yeni antimikrobiyal ilaçların geliştirilmesine acil ihtiyaç duyulmaktadır. Ayrıca kanser tedavisinde kullanmak amacıyla sentezlenen bileşiklerin kanserli hücrelerde olduğu kadar normal hücrelerde de sitotoksik etki göstermesi ve yan etkilerinin fazla olması nedeniyle kullanımını sınırlı hale getirmektedir. Bu nedenle kanser tedavisi için geliştirilen ilaçların seçici olarak kanserli hücreler üzerinde etkili olması ve yan etkilerinin mümkün olduğunca azaltılması yönünde araştırmalar yoğun olarak devam etmektedir. Bu çalışma kapsamında 2'si orjinal olmayan 7 tane 2-(p-substitutedphenyl)-5-(2-substitutedacetamido) bileşiğinin 3 basamakta sentezi gerçekleştirildi, yapıları HRMS, 1H-NMR ve 13C-NMR spektrokpisi yöntemleri ile aydınlatıldı. 10 farklı mikroorganizma üzerinde antimikrobiyal etkileri değerlendirildi. Ayrıca MCF-7 ve A549 hüzre hatları üzerinde MTT metodu ile sitotoksik aktiviteleri araştırıldı. Çalışma sonucunda sentezlenen bileşiklerin 64-256 µg/mL arasında değişen MİK değerlerinde hem antibakteriyel hem de antifungal etkili oldukarı belirlenmiş ve bileşik 7'nin hem A549 hem de MCF-7 üzerinde önemli sitotoksik aktivitesi bulunmuştur. Elde edilen sonuçlar yeni veya alternatif ilaç antimikrobiyal ve sitotoksik ajan geliştirmek için yapılan çalışmalara önemli bir katkı sağlayacaktır.