Zhi-Chuang Lü

Prof. Zhi-Chuang Lü

orcid

Research Focus & Metrics

Academic Career Length

7Years (2020-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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24 February 2023 in Environmental Science: Nano
Verified
Journal Article
Open access
PDF file
XWXiaodi Wang
Xiaodi Wang
SJShunxia Ji
Shunxia Ji
SBSiyan Bi
Siyan Bi
YTYanhong Tang
Yanhong Tang
GZGuifen Zhang
Guifen Zhang
WLWanxue Liu
Wanxue Liu
Nanocarrier-delivered dsRNA can be a promising environmentally friendly pest management solution.

21 March 2017 in PLOS ONE
Verified
Journal Article
Open access
PDF file
TDtian mei Dai
tian mei Dai
ZLZhichuang Lu
Zhichuang Lu
wanxue liuwanxue liu
wanxue liu
FWFanghao Wan
Fanghao Wan
The Bemisia tabaci Mediterranean (MED) cryptic species has been rapidly invading to most parts of the world owing to its strong ecological adaptability, which is considered as a model insect for stress tolerance studies under rapidly changing environments. Selection of a suitable reference gene for quantitative stress-responsive gene expression analysis based on qRT-PCR is critical for elaborating the molecular mechanisms of thermotolerance. To obtain accurate and reliable normalization data in MED, eight candidate reference genes (β-act, GAPDH, β-tub, EF1-α, GST, 18S, RPL13A and α-tub) were examined under various thermal stresses for varied time periods by using geNorm, NormFinder and BestKeeper algorithms, respectively. Our results revealed that β-tub and EF1-α were the best reference genes across all sample sets. On the other hand, 18S and GADPH showed the least stability for all the samples studied. β-act was proved to be highly stable only in case of short-term thermal stresses. To our knowledge this was the first comprehensive report on validation of reference genes under varying temperature stresses in MED. The study could expedite particular discovery of thermotolerance genes in MED. Further, the present results can form the basis of further research on suitable reference genes in this invasive insect and will facilitate transcript profiling in other invasive insects.

10 August 2020 in Marine Mammal Science
Verified
Journal Article
Closed access
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JTJiashen Tian
Jiashen Tian
JDJing Du
Jing Du
JHJiabo Han
Jiabo Han
XSXinran Song
Xinran Song
ZLZhichuang Lu
Zhichuang Lu

30 November 2021 in Insects
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Journal Article
Open access
PDF file
XSXiaona Shen
Xiaona Shen
wanxue liuwanxue liu
wanxue liu
FWFanghao Wan
Fanghao Wan
ZLZhi-Chuang Lü
Zhi-Chuang Lü
JGJianying Guo
Jianying Guo
The position of the chromatin opening of Bemisia tabaci undergoes significant changes under different temperature stresses, and numerous regulatory factors have been found. In this study, we verified two key factors, cytochrome P450 4C1 and carbonic anhydrase 3. The results showed that invasive whiteflies had a significantly lower heat resistance after silencing BtCYP 4C1 and BtCar3. In addition, whiteflies had a higher cold tolerance after silencing BtCYP 4C1. These results indicate that BtCYP 4C1 and BtCar3 are key regulators in the temperature adaptation of B. tabaci. Moreover, they may be key factors in influencing the geographical distribution and dispersal of B. tabaci as an invasive species in China.

5 April 2022 in Science of The Total Environment
Verified
Journal Article
Closed access
JTJiashen Tian
Jiashen Tian
Edmond SanganyadoEdmond Sanganyado
Edmond Sanganyado
ZWZhen Wang
Zhen Wang
ZKZhongren Kong
Zhongren Kong
JHJiabo Han
Jiabo Han
LWLiu Wenhua
Liu Wenhua

19 May 2022 in Frontiers in Genetics
Verified
Journal Article
Open access
SJShun-Xia Ji
Shun-Xia Ji
SBSi-Yan Bi
Si-Yan Bi
XWXiao-Di Wang
Xiao-Di Wang
QWQiang Wu
Qiang Wu
YTYan-Hong Tang
Yan-Hong Tang
WLWan-Xue Liu
Wan-Xue Liu
The tomato leaf miner Tuta absoluta (Meyrick) is one of the world’s most destructive pests of tomato, and because of its severe economic impacts, as well as the development of pesticide resistance, the species has been intensively studied, especially in regard to the identification of targets for T. absoluta control. However, functional genomic studies of T. absoluta have been constrained by a lack of effective genetic tools. Therefore, the aim of the present study was to develop a CRISPR/Cas9 zygote microinjection protocol for generating heritable mutations in T. absoluta, using the ommochrome synthesis gene cinnabar as an easily evaluated target gene. The injection of fertilised eggs with Cas9 protein and four sgRNAs, which targeted cinnabar exon 3, resulted in a mutagenesis rate of 31.9% for eggs reaching adulthood, and cinnabar mutagenesis resulted in either red or mosaic eye colour phenotypes. As such, this study is the first to report a complete and detailed CRISPR/Cas9 workflow for the efficient genome editing of the globally important invasive pest T. absoluta. The application of this robust genome-editing tool to T. absoluta will greatly facilitate the discovery of suitable RNAi control targets and the subsequent development of novel control strategies.