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Nephrotoxicity is the primary health effect of uranium exposure. However, the renal transport and intracellular behavior of uranium remains to be clearly elucidated. In the present study, the intracellular uranium distribution was examined with the cell lines derived from the S3 segment of mouse renal proximal tubules, which is a toxic target site of uranium, using microbeam-based elemental analysis. Uranium exposure at 100 μM for 24 h (non-toxic phase) was performed in S3 cells. Two types of measurement specimens, including those that are adhesive cell specimens and cryosection specimens, were examined for the positional relationship of the intracellular localization of uranium. Based on the combined results of single-cell imaging from the two types of cell specimens, uranium was distributed inside the cell and localized in the cytoplasm near the cell nucleus. In some cells, uranium was colocalized with phosphorus and potassium. The amount of uranium accumulated in S3 cells was estimated using thin section-standards. The mean uranium content of three adhesive cells was hundreds of femtogram per cell. Thus, we believe that single-cell imaging would be useful for studies on renal uranium transportation and cellular behavior.
Shino Homma-Takeda; Hitomi Fujishiro; Izumi Tanaka; Haruko Yakumaru; Kyoko Ayama; Akihiro Uehara; Masakazu Oikawa; Seiichiro Himeno; Hiroshi Ishihara. Single-Cell Imaging for Studies of Renal Uranium Transport and Intracellular Behavior. Minerals 2021, 11, 191 .
AMA StyleShino Homma-Takeda, Hitomi Fujishiro, Izumi Tanaka, Haruko Yakumaru, Kyoko Ayama, Akihiro Uehara, Masakazu Oikawa, Seiichiro Himeno, Hiroshi Ishihara. Single-Cell Imaging for Studies of Renal Uranium Transport and Intracellular Behavior. Minerals. 2021; 11 (2):191.
Chicago/Turabian StyleShino Homma-Takeda; Hitomi Fujishiro; Izumi Tanaka; Haruko Yakumaru; Kyoko Ayama; Akihiro Uehara; Masakazu Oikawa; Seiichiro Himeno; Hiroshi Ishihara. 2021. "Single-Cell Imaging for Studies of Renal Uranium Transport and Intracellular Behavior." Minerals 11, no. 2: 191.
Cadmium (Cd) is an environmental pollutant known to cause dysfunctions of the tubular reabsorption of biomolecules in the kidney. Elevated levels of urinary excretion of low-molecular-weight proteins such as β2-microglobulin (β2-MG) have been used as an indicator of Cd-induced renal tubular dysfunctions. However, very few studies have examined the direct effects of Cd on the reabsorption efficiency of proteins using cultured renal cells. Here, we developed an in vitro assay system for quantifying the endocytic uptakes of fluorescent-labeled proteins by flow cytometry in S1 and S2 cells derived from mouse kidney proximal tubules. Endocytic uptakes of fluorescent-labeled albumin, transferrin, β2-MG, and metallothionein into S1 cells were confirmed by fluorescence imaging and flow cytometry. The exposure of S1 and S2 cells to Cd at 1 and 3 µM for 3 days resulted in significant decreases in the uptakes of β2-MG and metallothionein but not in those of albumin or transferrin. These results suggest that Cd affects the tubular reabsorption of low-molecular-weight proteins even at nonlethal concentrations. The in vitro assay system developed in this study to evaluate the endocytic uptakes of proteins may serve as a useful tool for detecting toxicants that cause renal tubular dysfunctions.
Hitomi Fujishiro; Hazuki Yamamoto; Nobuki Otera; Nanae Oka; Mei Jinno; Seiichiro Himeno. In Vitro Evaluation of the Effects of Cadmium on Endocytic Uptakes of Proteins into Cultured Proximal Tubule Epithelial Cells. Toxics 2020, 8, 24 .
AMA StyleHitomi Fujishiro, Hazuki Yamamoto, Nobuki Otera, Nanae Oka, Mei Jinno, Seiichiro Himeno. In Vitro Evaluation of the Effects of Cadmium on Endocytic Uptakes of Proteins into Cultured Proximal Tubule Epithelial Cells. Toxics. 2020; 8 (2):24.
Chicago/Turabian StyleHitomi Fujishiro; Hazuki Yamamoto; Nobuki Otera; Nanae Oka; Mei Jinno; Seiichiro Himeno. 2020. "In Vitro Evaluation of the Effects of Cadmium on Endocytic Uptakes of Proteins into Cultured Proximal Tubule Epithelial Cells." Toxics 8, no. 2: 24.
The transport systems for metals play crucial roles in both the physiological functions of essential metals and the toxic effects of hazardous metals in mammals and plants. In mammalian cells, Zn transporters such as ZIP8 and ZIP14 have been found to function as the transporters for Mn(II) and Cd(II), contributing to the maintenance of Mn homeostasis and metallothionein-independent transports of Cd, respectively. In rice, the Mn transporter OsNramp5 expressed in the root is used for the uptake of Cd from the soil. Japan began to cultivate OsNramp5 mutant rice, which was found to accumulate little Cd, to prevent Cd accumulation. Inorganic trivalent arsenic (As(III)) is absorbed into mammalian cells via aquaglyceroporin, a water and glycerol channel. The ortholog of aquaporin in rice, OsLsi1, was found to be an Si transporter expressed in rice root, and is responsible for the absorption of soil As(III) into the root. Since rice is a hyperaccumulator of Si, higher amounts of As(III) are incorporated into rice compared to other plants. Thus, the transporters of essential metals are also utilized to incorporate toxic metals in both mammals and plants, and understanding the mechanisms of metal transports is important for the development of mitigation strategies against food contamination.
Seiichiro Himeno; Daigo Sumi; Hitomi Fujishiro. Toxicometallomics of Cadmium, Manganese and Arsenic with Special Reference to the Roles of Metal Transporters. Toxicological Research 2019, 35, 311 -317.
AMA StyleSeiichiro Himeno, Daigo Sumi, Hitomi Fujishiro. Toxicometallomics of Cadmium, Manganese and Arsenic with Special Reference to the Roles of Metal Transporters. Toxicological Research. 2019; 35 (4):311-317.
Chicago/Turabian StyleSeiichiro Himeno; Daigo Sumi; Hitomi Fujishiro. 2019. "Toxicometallomics of Cadmium, Manganese and Arsenic with Special Reference to the Roles of Metal Transporters." Toxicological Research 35, no. 4: 311-317.
The mechanisms underlying tissue cadmium (Cd) accumulation in mammals have long been investigated with a focus on the roles of metallothionein, a high-affinity Cd-binding protein. However, the precise mechanisms underlying the influx and efflux of Cd at the cell membrane have remained unclear. As Cd is not an essential element, the transport pathways for essential elements are thought to be utilized for Cd transport, especially for entry into cells. Recently, the roles of metal transporters for iron, calcium, zinc, and manganese in cellular Cd transport have begun to be elucidated. The expression and roles of these transporters vary depending on the tissue or cell types and the nutritional statuses of the elements. Consequently, the usage of metal transporters for Cd transport is also altered depending on the tissue and nutritional status of an element. In this chapter, recent advances in understanding the mechanisms underlying cellular Cd transport from the standpoint of the roles of metal transporters are discussed.
Seiichiro Himeno; Hitomi Fujishiro. Roles of Metal Transporters in Cellular Cadmium Transport in Mammals. Current Topics in Environmental Health and Preventive Medicine 2019, 163 -178.
AMA StyleSeiichiro Himeno, Hitomi Fujishiro. Roles of Metal Transporters in Cellular Cadmium Transport in Mammals. Current Topics in Environmental Health and Preventive Medicine. 2019; ():163-178.
Chicago/Turabian StyleSeiichiro Himeno; Hitomi Fujishiro. 2019. "Roles of Metal Transporters in Cellular Cadmium Transport in Mammals." Current Topics in Environmental Health and Preventive Medicine , no. : 163-178.
Groundwater particularly drinking water contamination with metals has created an environmental disaster in Bangladesh. Manganese (Mn), an essential trace element, plays a key role in the development and function of the brain. Excess Mn exposure is reported to be associated with complex neurological disorders. Here, we have found a notably large extent of Mn above the permissive limit in the tube-well water of Rajshahi and Naogaon districts in Bangladesh. Higher levels of Mn in hair and nail samples, and a decreasing level of butyrylcholinesterase (BChE) activity were detected in plasma samples of the human subjects recruited from Naogaon district. Mn concentrations in water, hair, and nails were negatively correlated with the plasma BChE levels in Mn-exposed populations. To compare and validate these human studies, an animal model was used to determine the in vivo effects of Mn on neurobehavioral changes and blood BChE levels. In elevated plus maze, the time spent was significantly reduced in open arms and increased in closed arms of Mn-exposed mice compared to control group. The mean latency time to find the platform was declined significantly in control mice compared to Mn-treated group during 7 days in Morris water maze test, and Mn-exposed group also spent significantly less time in the desired quadrant as compared to the control group in probe trial. BChE activity was significantly reduced in Mn-exposed mice compared to control mice. Taken together, these results suggest that plasma BChE levels may serve as reliable biomarker of Mn-induced neurotoxicity related to behavioral changes.
Adiba Anjum; Sheta Biswas; Mizanur Rahman; Atiqur Rahman; Abu Eabrahim Siddique; Yeasir Karim; Sharmin Aktar; Farjana Nikkon; Azizul Haque; Seiichiro Himeno; Khaled Hossain; Zahangir Alam Saud. Butyrylcholinesterase—a potential plasma biomarker in manganese-induced neurobehavioral changes. Environmental Science and Pollution Research 2019, 26, 6378 -6387.
AMA StyleAdiba Anjum, Sheta Biswas, Mizanur Rahman, Atiqur Rahman, Abu Eabrahim Siddique, Yeasir Karim, Sharmin Aktar, Farjana Nikkon, Azizul Haque, Seiichiro Himeno, Khaled Hossain, Zahangir Alam Saud. Butyrylcholinesterase—a potential plasma biomarker in manganese-induced neurobehavioral changes. Environmental Science and Pollution Research. 2019; 26 (7):6378-6387.
Chicago/Turabian StyleAdiba Anjum; Sheta Biswas; Mizanur Rahman; Atiqur Rahman; Abu Eabrahim Siddique; Yeasir Karim; Sharmin Aktar; Farjana Nikkon; Azizul Haque; Seiichiro Himeno; Khaled Hossain; Zahangir Alam Saud. 2019. "Butyrylcholinesterase—a potential plasma biomarker in manganese-induced neurobehavioral changes." Environmental Science and Pollution Research 26, no. 7: 6378-6387.
Groundwater used for drinking has been contaminated with naturally occurring inorganic arsenic and other metals, and metal-contaminated drinking water is the biggest threat to public health in Bangladesh. Toxic metals present in the drinking water have a strong relationship with chronic diseases in humans. Antimony (Sb), a naturally occurring metal, has been reported to be present in the drinking water along with other heavy metals in Bangladesh. Although Sb is present in the environment, very little attention has been given to the toxic effects of Sb. The present study was designed to investigate the in vivo effects of Sb on neurobehavioral changes like anxiety, learning and memory impairment, and blood indices related to organ dysfunction. Mice exposed to antimony potassium-tartrate hydrate (Sb) (10 mg/kg body weight) significantly (p < 0.05) decreased the time spent in open arms while increased the time spent in closed arms compared to the control mice in elevated plus maze. The mean latency time of control group to find the platform decreased (p < 0.05) significantly during 7 days learning as compared to Sb-treated group in Morris water maze test, and Sb-exposed group spent significantly (p < 0.05) less time in the desired quadrant as compared to the control group in probe trial. Sb treatment also significantly altered blood indices related to liver and kidney dysfunction. Additionally, Sb-induced biochemical alterations were associated with significant perturbations in histological architecture of liver and kidney of Sb-exposed mice. These data suggest that Sb has a toxic effect on neurobehavioral and biochemical changes in mice.
Tanzina Tanu; Adiba Anjum; Momotaj Jahan; Farjana Nikkon; Mominul Hoque; Apurba Kumar Roy; Azizul Haque; Seiichiro Himeno; Khaled Hossain; Zahangir Alam Saud. Antimony-Induced Neurobehavioral and Biochemical Perturbations in Mice. Biological Trace Element Research 2018, 186, 199 -207.
AMA StyleTanzina Tanu, Adiba Anjum, Momotaj Jahan, Farjana Nikkon, Mominul Hoque, Apurba Kumar Roy, Azizul Haque, Seiichiro Himeno, Khaled Hossain, Zahangir Alam Saud. Antimony-Induced Neurobehavioral and Biochemical Perturbations in Mice. Biological Trace Element Research. 2018; 186 (1):199-207.
Chicago/Turabian StyleTanzina Tanu; Adiba Anjum; Momotaj Jahan; Farjana Nikkon; Mominul Hoque; Apurba Kumar Roy; Azizul Haque; Seiichiro Himeno; Khaled Hossain; Zahangir Alam Saud. 2018. "Antimony-Induced Neurobehavioral and Biochemical Perturbations in Mice." Biological Trace Element Research 186, no. 1: 199-207.
Chronic exposure to arsenic is associated with increased morbidity and mortality from cardiovascular disease (CVD); however, plausible biomarker for early prediction and the underlying mechanism of arsenic-related CVD have not yet been clearly understood. Endothelial dysfunction plays a central role in the development of CVD. We hypothesized that endothelial damage or dysfunction is an important aspect and may be an early event of arsenic-related CVD. Soluble thrombomodulin (sTM) in serum is thought to be a specific and stable marker for endothelial damage or dysfunction. This study was designed to evaluate the association between chronic exposure to arsenic and sTM among human subjects in arsenic-endemic and non-endemic rural areas in Bangladesh. A total of 321 study subjects (217 from arsenic-endemic areas and 104 from a non-endemic area) were recruited. Subjects’ arsenic exposure levels (i.e., drinking water, hair and nail arsenic concentrations) were measured by Inductively Coupled Plasma Mass Spectroscopy. The subjects’ serum sTM levels were quantified by immunoassay kit. The average sTM levels of the subjects in arsenic-endemic and non-endemic areas were 4.58 ± 2.20 and 2.84 ± 1.29 (ng mL-1) respectively, and the difference was significant (p<0.001). Arsenic exposure levels showed a significant (water arsenic: rs = 0.339, p<0.001, hair arsenic: rs = 0.352, p<0.001 and nail arsenic: rs = 0.308, p<0.001) positive associations with sTM levels. Soluble TM levels were higher in the higher exposure gradients if we stratified the subjects into tertile groups (low, medium and high) based on the arsenic concentrations of the subjects’ drinking water, hair and nails. Finally, increased levels of sTM were negatively correlated with high density lipoprotein cholesterol (HDL-C), and positively correlated with intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1). Results of this study show that chronic exposure to arsenic has mild to moderate association with sTM levels.
M. M. Hasibuzzaman; Shakhawoat Hossain; Shofikul Islam; Atiqur Rahman; Adiba Anjum; Faruk Hossain; Nayan Chandra Mohanto; Rezaul Karim; Mominul Hoque; Zahangir Alam Saud; Hideki Miyataka; Seiichiro Himeno; Khaled Hossain. Association between arsenic exposure and soluble thrombomodulin: A cross sectional study in Bangladesh. PLoS ONE 2017, 12, e0175154 .
AMA StyleM. M. Hasibuzzaman, Shakhawoat Hossain, Shofikul Islam, Atiqur Rahman, Adiba Anjum, Faruk Hossain, Nayan Chandra Mohanto, Rezaul Karim, Mominul Hoque, Zahangir Alam Saud, Hideki Miyataka, Seiichiro Himeno, Khaled Hossain. Association between arsenic exposure and soluble thrombomodulin: A cross sectional study in Bangladesh. PLoS ONE. 2017; 12 (4):e0175154.
Chicago/Turabian StyleM. M. Hasibuzzaman; Shakhawoat Hossain; Shofikul Islam; Atiqur Rahman; Adiba Anjum; Faruk Hossain; Nayan Chandra Mohanto; Rezaul Karim; Mominul Hoque; Zahangir Alam Saud; Hideki Miyataka; Seiichiro Himeno; Khaled Hossain. 2017. "Association between arsenic exposure and soluble thrombomodulin: A cross sectional study in Bangladesh." PLoS ONE 12, no. 4: e0175154.
Chronic exposure to arsenic is associated with cancer and hypertension. Growing evidence suggests that altered methylation in long interspersed nuclear element-1 (LINE-1) is involved in many types of disorders, including cardiovascular disease. Here we evaluated the association between arsenic exposure and LINE-1 methylation levels, especially in relation to blood pressure (BP). A total of 236 subjects (175 from arsenic-endemic areas and 61 from a non-endemic area) in rural Bangladesh were recruited. The subjects’ arsenic exposure levels (i.e., drinking water, hair and nail arsenic concentrations) were measured by inductively coupled plasma mass spectroscopy. The subjects’ LINE-1 methylation levels were determined by pyrosequencing. The average LINE-1 methylation levels of the subjects living in the arsenic-endemic areas were significantly (p < 0.01) lower than those of the subjects living in the non-endemic area. In a sex-stratified analysis, the arsenic exposure levels in female but not male subjects showed a significant inverse association with LINE-1 methylation levels before (water arsenic: p < 0.01, hair arsenic: p < 0.05, nail arsenic: p < 0.001) and after (water arsenic: p < 0.01, hair arsenic: p < 0.05, nail arsenic: p < 0.001) adjustment for age, body mass index and smoking. Analyses examining interactions among arsenic levels, BP and LINE-1 methylation showed that arsenic-related elevated levels of BP were associated with LINE-1 hypomethylation. Our findings demonstrated that chronic exposure to arsenic was inversely associated with LINE-1 methylation levels in blood leukocyte DNA and this was more pronounced in females than males; in addition, the decreased levels of LINE-1 methylation might be involved in the arsenic-induced elevation of BP.
Khaled Hossain; Takehiro Suzuki; M. M. Hasibuzzaman; Shofikul Islam; Atiqur Rahman; Sudip Kumar Paul; Tanzina Tanu; Shakhawoat Hossain; Zahangir Alam Saud; Mashiur Rahman; Farjana Nikkon; Hideki Miyataka; Seiichiro Himeno; Keiko Nohara. Chronic exposure to arsenic, LINE-1 hypomethylation, and blood pressure: a cross-sectional study in Bangladesh. Environmental Health 2017, 16, 1 -12.
AMA StyleKhaled Hossain, Takehiro Suzuki, M. M. Hasibuzzaman, Shofikul Islam, Atiqur Rahman, Sudip Kumar Paul, Tanzina Tanu, Shakhawoat Hossain, Zahangir Alam Saud, Mashiur Rahman, Farjana Nikkon, Hideki Miyataka, Seiichiro Himeno, Keiko Nohara. Chronic exposure to arsenic, LINE-1 hypomethylation, and blood pressure: a cross-sectional study in Bangladesh. Environmental Health. 2017; 16 (1):1-12.
Chicago/Turabian StyleKhaled Hossain; Takehiro Suzuki; M. M. Hasibuzzaman; Shofikul Islam; Atiqur Rahman; Sudip Kumar Paul; Tanzina Tanu; Shakhawoat Hossain; Zahangir Alam Saud; Mashiur Rahman; Farjana Nikkon; Hideki Miyataka; Seiichiro Himeno; Keiko Nohara. 2017. "Chronic exposure to arsenic, LINE-1 hypomethylation, and blood pressure: a cross-sectional study in Bangladesh." Environmental Health 16, no. 1: 1-12.
As cadmium (Cd) is a nonessential metal, there is no specific transport system for cellular entry of Cd in the organisms. The establishment of Cd-resistant cells from metallothionein-null mouse cells, application of multi-tracer technique, and microarray analyses have revealed that Cd2+ shares the pathway for cellular incorporation with Mn2+, and the responsible transporters for this pathway were found to be ZIP8 (Zrt- and Irt-related protein 8) and ZIP14. Although other transport systems for iron or calcium are also utilized for cellular incorporation of Cd2+ and Mn2+, characterization of ZIP8 and ZIP14 has demonstrated important physiological and pathological roles of these transporters in metal transport. We show here the significant roles of ZIP8 in segment-specific transport of Cd in proximal tubule of the kidney and the roles of ZIP14 and ZnT10 in Mn transport in neuronal cells in the presence of cytokine. Recently, critical roles of Mn transport systems have been highlighted by the findings of human diseases related to the mutation in ZIP8 and ZnT10. This chapter summarized historical background and recent advances in the studies on the roles of ZIP8 and ZIP14 in the transport of Cd2+ and Mn2+.
Seiichiro Himeno; Hitomi Fujishiro. Roles of Zinc Transporters in Cellular Transport of Cadmium and Manganese. Chemistry and Biology of Pteridines and Folates 2017, 265 -283.
AMA StyleSeiichiro Himeno, Hitomi Fujishiro. Roles of Zinc Transporters in Cellular Transport of Cadmium and Manganese. Chemistry and Biology of Pteridines and Folates. 2017; ():265-283.
Chicago/Turabian StyleSeiichiro Himeno; Hitomi Fujishiro. 2017. "Roles of Zinc Transporters in Cellular Transport of Cadmium and Manganese." Chemistry and Biology of Pteridines and Folates , no. : 265-283.
Intestinal absorption of cadmium (Cd) is considered to be mediated mainly by the ferrous iron transporter DMT1, or the calcium transporter CaT1. The roles of zinc transporters such as ZIP8 and ZIP14 remain unclear, and the roles of these four transporters in the intestinal uptake of Cd under physiological conditions have not been compared. Here, we used a trans-well cell culture system to investigate the effects of the down-regulation of these four transporters on the uptake of Cd from the apical side of enterocytes. We used a Caco-2-kh cell line that can form tight junctions within a few days. The transfection of DMT1 siRNA significantly decreased the Cd uptake from the apical side at 5 μM, but not at 0.1 or 1 μM. The transfection of ZIP14 siRNA markedly decreased the Cd uptake at 0.1 and 1 μM, but not at 5 μM. The transfection of siRNA of CaT1 or ZIP8 did not alter the Cd uptake at any concentrations of Cd examined. These results suggest that DMT1 and ZIP14 play different roles in intestinal Cd absorption depending on the concentration of Cd.
Hitomi Fujishiro; Satoko Hamao; Rina Tanaka; Taiho Kambe; Seiichiro Himeno. Concentration-dependent roles of DMT1 and ZIP14 in cadmium absorption in Caco-2 cells. The Journal of Toxicological Sciences 2017, 42, 559 -567.
AMA StyleHitomi Fujishiro, Satoko Hamao, Rina Tanaka, Taiho Kambe, Seiichiro Himeno. Concentration-dependent roles of DMT1 and ZIP14 in cadmium absorption in Caco-2 cells. The Journal of Toxicological Sciences. 2017; 42 (5):559-567.
Chicago/Turabian StyleHitomi Fujishiro; Satoko Hamao; Rina Tanaka; Taiho Kambe; Seiichiro Himeno. 2017. "Concentration-dependent roles of DMT1 and ZIP14 in cadmium absorption in Caco-2 cells." The Journal of Toxicological Sciences 42, no. 5: 559-567.
Fish is the main source of animal protein and micronutrients for inhabitants in the lower Mekong River basin. Consumption of fish in the basin ranges from 41 to 51 kg capita(-1) year(-1). Thus, concerns of human health impacts caused by daily intake of metals contained in fish, and the incidence of bacterial contamination from Listeria and Escherichia coli have been raised. This study was conducted to 1) determine concentrations of metals, fecal indicator organisms, and Listeria spp. in cultivated common diet fish, and 2) assess human health risks as results of fish consumption on a daily basis. The results showed significant impacts of metal accumulation in fish especially from the intensive aquaculture. Chemical use to promote the rapid allometric growth of fish was expected to be the explanation for this finding. Concentrations of metals contained in different fish species were not statistically different with the exceptions of Na, Mn, and Zn. This might be due to the mobility of elements in aquaculture farms. Listeria and E. coli log CFU/g were 1.36 ± 0.11 (standard error) and 1.57 ± 0.1 s.e., respectively with higher counts observed in samples collected in market sites. Lastly, for human health risk assessment via fish consumption, it was found that hazard quotients of consuming As, Cu, and Zn contained in all fish species could contribute adverse health effects to the local residents (hazard quotients higher than 1). Therefore, risk management measures must be promoted and implemented in all study areas to reduce potential risks to local Vietnamese residents.
Penradee Chanpiwat; Suthipong Sthiannopkao; Kenneth Widmer; Seiichiro Himeno; Hideki Miyataka; Ngoc-Ut Vu; Van-Viet Tran; Thi-Tuyet-Ngan Pham. Assessment of metal and bacterial contamination in cultivated fish and impact on human health for residents living in the Mekong Delta. Chemosphere 2016, 163, 342 -350.
AMA StylePenradee Chanpiwat, Suthipong Sthiannopkao, Kenneth Widmer, Seiichiro Himeno, Hideki Miyataka, Ngoc-Ut Vu, Van-Viet Tran, Thi-Tuyet-Ngan Pham. Assessment of metal and bacterial contamination in cultivated fish and impact on human health for residents living in the Mekong Delta. Chemosphere. 2016; 163 ():342-350.
Chicago/Turabian StylePenradee Chanpiwat; Suthipong Sthiannopkao; Kenneth Widmer; Seiichiro Himeno; Hideki Miyataka; Ngoc-Ut Vu; Van-Viet Tran; Thi-Tuyet-Ngan Pham. 2016. "Assessment of metal and bacterial contamination in cultivated fish and impact on human health for residents living in the Mekong Delta." Chemosphere 163, no. : 342-350.
Arsenic (As) toxicity has caused an environmental tragedy affecting millions of people in the world. Little is known about the toxic effects of As on neurobehavioral and biochemical changes in vivo. Along this line of metal toxicity, co-exposure of lead (Pb) could aggravate the situation in the host. The present study was designed to explore the combined effects of As and Pb on behavioral changes like anxiety, spatial memory and learning impairment, and blood indices related to organ dysfunction. Exposure of mice to As (10 mg/kg body weight), Pb (10 mg/kg body weight), and As + Pb via drinking water significantly decreased the time spent exploring the open arms while it increased the time spent in the closed arms compared to control mice in the elevated plus maze. The mean latency time of the control group to find the platform decreased significantly during the learning for 7 days compared to all three treated groups in the Morris water maze test, and the As-exposed group spent significantly less time in the desired quadrant as compared to the control group in the probe trial. Both metals posed an anxiety-like behavior and deficits in spatial memory and learning, and also altered blood indices related to liver and kidney dysfunction, and a combined exposure of these metals inhibited the individual accumulation of As and Pb. Taken together, these data suggest that As has more toxic effects on neurobehavioral and biochemical changes than Pb, and there may be antagonism in the effects and accumulation between these two toxicants.
Sharmin Aktar; Momotaj Jahan; Shahnur Alam; Nayan Chandra Mohanto; Afroza Arefin; Atiqur Rahman; Azizul Haque; Seiichiro Himeno; Khaled Hossain; Zahangir Alam Saud. Individual and Combined Effects of Arsenic and Lead on Behavioral and Biochemical Changes in Mice. Biological Trace Element Research 2016, 177, 288 -296.
AMA StyleSharmin Aktar, Momotaj Jahan, Shahnur Alam, Nayan Chandra Mohanto, Afroza Arefin, Atiqur Rahman, Azizul Haque, Seiichiro Himeno, Khaled Hossain, Zahangir Alam Saud. Individual and Combined Effects of Arsenic and Lead on Behavioral and Biochemical Changes in Mice. Biological Trace Element Research. 2016; 177 (2):288-296.
Chicago/Turabian StyleSharmin Aktar; Momotaj Jahan; Shahnur Alam; Nayan Chandra Mohanto; Afroza Arefin; Atiqur Rahman; Azizul Haque; Seiichiro Himeno; Khaled Hossain; Zahangir Alam Saud. 2016. "Individual and Combined Effects of Arsenic and Lead on Behavioral and Biochemical Changes in Mice." Biological Trace Element Research 177, no. 2: 288-296.
We previously reported that two splicing variants of human AS3MT mRNA, exon-3 skipping form (Δ3) and exons-4 and -5 skipping form (Δ4,5), were detected in HepG2 cells and that both variants lacked arsenic methylation activity (Sumi et al., 2011). Here we studied whether hydrogen peroxide (H2O2) triggers alternative splicing of AS3MT mRNA. The results showed that exposure of HepG2 cells to H2O2 resulted in increased levels of a novel spliced form skipping exon-3 to exon-10 (Δ3-10) in an H2O2-concentration-dependent manner, although no change was detected in the mRNA levels of Δ3 AS3MT. We found decreased protein levels of serine/arginine-rich 40 (SRp40), which we determined to be a candidate splice factor for controlling the splicing of AS3MT mRNA. We next compared the amounts of methylated arsenic metabolites between control and H2O2-exposed HepG2 cells after the addition of arsenite as a substance. The results showed lower levels of methylated arsenic metabolites in HepG2 cells exposed to H2O2. These data suggest that the splicing of AS3MT pre-mRNA was disconcerted by oxidative stress and that abnormal alternative splicing of AS3MT mRNA may affect arsenic methylation ability.
Daigo Sumi; Chieri Takeda; Daiki Yasuoka; Seiichiro Himeno. Hydrogen peroxide triggers a novel alternative splicing of arsenic (+3 oxidation state) methyltransferase gene. Biochemical and Biophysical Research Communications 2016, 480, 18 -22.
AMA StyleDaigo Sumi, Chieri Takeda, Daiki Yasuoka, Seiichiro Himeno. Hydrogen peroxide triggers a novel alternative splicing of arsenic (+3 oxidation state) methyltransferase gene. Biochemical and Biophysical Research Communications. 2016; 480 (1):18-22.
Chicago/Turabian StyleDaigo Sumi; Chieri Takeda; Daiki Yasuoka; Seiichiro Himeno. 2016. "Hydrogen peroxide triggers a novel alternative splicing of arsenic (+3 oxidation state) methyltransferase gene." Biochemical and Biophysical Research Communications 480, no. 1: 18-22.
Manganese (Mn) homeostasis involves coordinated regulation of specific proteins involved in Mn influx and efflux. However, the proteins that are involved in detoxification/efflux have not been completely resolved, nor has the basis by which they select their metal substrate. Here, we compared six proteins, which were reported to be involved in Mn detoxification/efflux, by evaluating their ability to reduce Mn toxicity in chicken DT40 cells, finding that human ZnT10 (hZnT10) was the most significant contributor. A domain swapping and substitution analysis between hZnT10 and a zinc-specific transporter hZnT1 showed that residue N43, which corresponds to the His residue constituting the potential intramembranous zinc coordination site in other ZnT transporters, is necessary to impart hZnT10's unique Mn mobilization activity; residues C52 and L242 in transmembrane domains II and V play a subtler role in controlling the metal specificity of hZnT10. Interestingly, the H->N reversion mutant in hZnT1 conferred Mn transport activity and loss of zinc transport activity. These results provide important information about Mn detoxification/efflux mechanisms in vertebrate cells as well as the molecular characterization of hZnT10 as a Mn transporter.
Yukina Nishito; Natsuko Tsuji; Hitomi Fujishiro; Taka-Aki Takeda; Tomohiro Yamazaki; Fumie Teranishi; Fumiko Okazaki; Ayu Matsunaga; Karin Tuschl; Rajini Rao; Satoshi Kono; Hiroaki Miyajima; Hiroshi Narita; Seiichiro Himeno; Taiho Kambe. Direct Comparison of Manganese Detoxification/Efflux Proteins and Molecular Characterization of ZnT10 Protein as a Manganese Transporter. Journal of Biological Chemistry 2016, 291, 14773 -14787.
AMA StyleYukina Nishito, Natsuko Tsuji, Hitomi Fujishiro, Taka-Aki Takeda, Tomohiro Yamazaki, Fumie Teranishi, Fumiko Okazaki, Ayu Matsunaga, Karin Tuschl, Rajini Rao, Satoshi Kono, Hiroaki Miyajima, Hiroshi Narita, Seiichiro Himeno, Taiho Kambe. Direct Comparison of Manganese Detoxification/Efflux Proteins and Molecular Characterization of ZnT10 Protein as a Manganese Transporter. Journal of Biological Chemistry. 2016; 291 (28):14773-14787.
Chicago/Turabian StyleYukina Nishito; Natsuko Tsuji; Hitomi Fujishiro; Taka-Aki Takeda; Tomohiro Yamazaki; Fumie Teranishi; Fumiko Okazaki; Ayu Matsunaga; Karin Tuschl; Rajini Rao; Satoshi Kono; Hiroaki Miyajima; Hiroshi Narita; Seiichiro Himeno; Taiho Kambe. 2016. "Direct Comparison of Manganese Detoxification/Efflux Proteins and Molecular Characterization of ZnT10 Protein as a Manganese Transporter." Journal of Biological Chemistry 291, no. 28: 14773-14787.
K Hossain; M Rahman; S Hossain; M Karim; M Islam; S Himeno. Associations of chronic arsenic exposure with circulating biomarkers of cardiovascular diseases. Arsenic in the Environment - Proceedings 2016, 368 -369.
AMA StyleK Hossain, M Rahman, S Hossain, M Karim, M Islam, S Himeno. Associations of chronic arsenic exposure with circulating biomarkers of cardiovascular diseases. Arsenic in the Environment - Proceedings. 2016; ():368-369.
Chicago/Turabian StyleK Hossain; M Rahman; S Hossain; M Karim; M Islam; S Himeno. 2016. "Associations of chronic arsenic exposure with circulating biomarkers of cardiovascular diseases." Arsenic in the Environment - Proceedings , no. : 368-369.
Seiichiro Himeno; C Tohmori; D Sumi; H Miyataka; S Sthiannopkao. Assessment of arsenic exposure among the residents living along the Mekong River in Cambodia. Arsenic in the Environment - Proceedings 2016, 390 -391.
AMA StyleSeiichiro Himeno, C Tohmori, D Sumi, H Miyataka, S Sthiannopkao. Assessment of arsenic exposure among the residents living along the Mekong River in Cambodia. Arsenic in the Environment - Proceedings. 2016; ():390-391.
Chicago/Turabian StyleSeiichiro Himeno; C Tohmori; D Sumi; H Miyataka; S Sthiannopkao. 2016. "Assessment of arsenic exposure among the residents living along the Mekong River in Cambodia." Arsenic in the Environment - Proceedings , no. : 390-391.
Dietary intake of macro-, trace and toxic elements was determined among the young adult population of Eastern Poland. The study was performed in 2011-2013 and involved 583 participants living in Lublin and its province. Dietary intakes of metals were determined using a 24-hour dietary recall technique and a market basket method. The analytical quantification of As, Ca, Cd, Cu, Fe, K, Mg, Mn, Mo, Na, Ni, Pb, Se and Zn was performed using ICP-MS technique, whereas the content of mercury was determined using the Mercury Analyzer. Performed investigations revealed that daily dietary intakes of the majority of the study elements (macro- and trace) and toxic elements such as As, Pb or Hg are within the range of reference values. However, high consumption of Na and improper Na/K ratio combined with low intake of Mg may be harmful to the health of the population. Moreover, obtained data suggest that the risk of developing diseases among population in Eastern Poland related to high exposure to Ni and Cd absorbed from foodstuffs was high.
Wojciech Koch; Rezaul Karim; Zbigniew Marzec; Hideki Miyataka; Seiichiro Himeno; Yoshinori Asakawa. Dietary intake of metals by the young adult population of Eastern Poland: Results from a market basket study. Journal of Trace Elements in Medicine and Biology 2016, 35, 36 -42.
AMA StyleWojciech Koch, Rezaul Karim, Zbigniew Marzec, Hideki Miyataka, Seiichiro Himeno, Yoshinori Asakawa. Dietary intake of metals by the young adult population of Eastern Poland: Results from a market basket study. Journal of Trace Elements in Medicine and Biology. 2016; 35 ():36-42.
Chicago/Turabian StyleWojciech Koch; Rezaul Karim; Zbigniew Marzec; Hideki Miyataka; Seiichiro Himeno; Yoshinori Asakawa. 2016. "Dietary intake of metals by the young adult population of Eastern Poland: Results from a market basket study." Journal of Trace Elements in Medicine and Biology 35, no. : 36-42.
AimsAll-trans retinoic acid (ATRA) is used to treat patients with acute promyelocytic leukemia (APL) due to its ability to resume the differentiation of APL cells. Recently, clinical trials have been started to evaluate ATRA plus arsenic trioxide (ATO) as a combination treatment for APL patients. However, little is known about the detailed mechanisms underlying its efficacy. We therefore investigated the effects of this combination on the differentiation and differentiation-related gene expression.Main methodsHuman leukemia HL-60 cells differentiation was examined using nitro blue tetrazolium and CD11b. The levels of mRNA and protein were determined by RT-qPCR, microarray, western blot and ELISA, respectively. The promoter activity was assessed by luciferase activity. The arsenic concentration was determined by ICP-MS.Key findingsATRA-induced HL-60 differentiation was augmented by co-treatment with ATO. A microarray analysis showed that ATRA plus ATO treatment markedly down-regulated the expression of proteinase 3 (PRTN3), which is involved in the differentiation arrest of leukemia cells, compared with treatment with ATRA alone. The PRTN3 mRNA level was suppressed by treatment with ATRA alone, and then further suppressed by co-treatment with ATO, accompanied by a concomitant increase in Sp1 protein, which is known to facilitate differentiation. The expression levels of azurocidin, telomerase reverse transcriptase, ferritin, and interleukin-1β were also altered by co-treatment with ATO.SignificanceCo-treatment with ATO enhances ATRA-induced HL-60 differentiation by altering the expression of genes involved in cell differentiation, providing the molecular basis for a combination therapy using ATO plus ATRA to treat leukemia patients.
Daigo Sumi; Kazumi Suzukawa; Seiichiro Himeno. Arsenic trioxide augments all-trans retinoic acid-induced differentiation of HL-60 cells. Life Sciences 2016, 149, 42 -50.
AMA StyleDaigo Sumi, Kazumi Suzukawa, Seiichiro Himeno. Arsenic trioxide augments all-trans retinoic acid-induced differentiation of HL-60 cells. Life Sciences. 2016; 149 ():42-50.
Chicago/Turabian StyleDaigo Sumi; Kazumi Suzukawa; Seiichiro Himeno. 2016. "Arsenic trioxide augments all-trans retinoic acid-induced differentiation of HL-60 cells." Life Sciences 149, no. : 42-50.
Cardiovascular diseases (CVDs) and cancers are the major causes of chronic arsenic exposure-related morbidity and mortality. Matrix metalloproteinase-2 (MMP-2) and −9 (MMP-9) are deeply involved in the pathogenesis of CVDs and cancers. This study has been designed to evaluate the interactions of arsenic exposure with serum MMP-2 and MMP-9 concentrations especially in relation to the circulating biomarkers of CVDs. A total of 373 human subjects, 265 from arsenic-endemic and 108 from non-endemic areas in Bangladesh were recruited for this study. Arsenic concentrations in the specimens were measured by inductively coupled plasma mass spectroscopy (ICP-MS) and serum MMPs were quantified by immunoassay kits. Serum MMP-2 and MMP-9 concentrations in arsenic-endemic population were significantly (p < 0.001) higher than those in non-endemic population. Both MMPs showed significant positive interactions with drinking water (r s = 0.208, p < 0.001 for MMP-2; r s = 0.163, p < 0.01 for MMP-9), hair (r s = 0.163, p < 0.01 for MMP-2; r s = 0.173, p < 0.01 for MMP-9) and nail (r s = 0.160, p < 0.01 for MMP-2; r s = 0.182, p < 0.001 for MMP-9) arsenic of the study subjects. MMP-2 concentrations were 1.02, 1.03 and 1.05 times, and MMP-9 concentrations were 1.03, 1.06 and 1.07 times greater for 1 unit increase in log-transformed water, hair and nail arsenic concentrations, respectively, after adjusting for covariates (age, sex, BMI, smoking habit and hypertension). Furthermore, both MMPs were increased dose-dependently when the study subjects were split into three (≤10, 10.1-50 and > 50 μg/L) groups based on the regulatory upper limit of water arsenic concentration set by WHO and Bangladesh Government. MMPs were also found to be significantly (p < 0.05) associated with each other. Finally, the concentrations of both MMPs were correlated with several circulating markers related to CVDs. This study showed the significant positive associations and dose–response relationships of arsenic exposure with serum MMP-2 and MMP-9 concentrations. This study also showed the interactions of MMP-2 and MMP-9 concentrations with the circulating markers of CVDs suggesting the MMP-2 and MMP-9 -mediated mechanism of arsenic-induced CVDs.
Shofikul Islam; Nayan Chandra Mohanto; Rezaul Karim; Sharmin Aktar; Mominul Hoque; Atiqur Rahman; Momotaj Jahan; Rabeya Khatun; Abdul Aziz; Kazi Abdus Salam; Zahangir Alam Saud; Mostaque Hossain; Aminur Rahman; Abul Mandal; Azizul Haque; Hideki Miyataka; Seiichiro Himeno; Khaled Hossain. Elevated concentrations of serum matrix metalloproteinase-2 and -9 and their associations with circulating markers of cardiovascular diseases in chronic arsenic-exposed individuals. Environmental Health 2015, 14, 92 .
AMA StyleShofikul Islam, Nayan Chandra Mohanto, Rezaul Karim, Sharmin Aktar, Mominul Hoque, Atiqur Rahman, Momotaj Jahan, Rabeya Khatun, Abdul Aziz, Kazi Abdus Salam, Zahangir Alam Saud, Mostaque Hossain, Aminur Rahman, Abul Mandal, Azizul Haque, Hideki Miyataka, Seiichiro Himeno, Khaled Hossain. Elevated concentrations of serum matrix metalloproteinase-2 and -9 and their associations with circulating markers of cardiovascular diseases in chronic arsenic-exposed individuals. Environmental Health. 2015; 14 (1):92.
Chicago/Turabian StyleShofikul Islam; Nayan Chandra Mohanto; Rezaul Karim; Sharmin Aktar; Mominul Hoque; Atiqur Rahman; Momotaj Jahan; Rabeya Khatun; Abdul Aziz; Kazi Abdus Salam; Zahangir Alam Saud; Mostaque Hossain; Aminur Rahman; Abul Mandal; Azizul Haque; Hideki Miyataka; Seiichiro Himeno; Khaled Hossain. 2015. "Elevated concentrations of serum matrix metalloproteinase-2 and -9 and their associations with circulating markers of cardiovascular diseases in chronic arsenic-exposed individuals." Environmental Health 14, no. 1: 92.
Chemical analyses of metal (Cr, Mn, Fe, Co, Ni, Cu, Zn, As, Mo, Ba, and Pb) concentrations in hair, nails, and urine of Cambodians in arsenic-contaminated areas who consumed groundwater daily showed elevated levels in these biomarkers for most metals of toxicological interest. The levels of metals in biomarkers corresponded to their levels in groundwater, especially for As, whose concentrations exceeded the WHO guidelines for drinking water. About 75.6% of hair samples from the population in this study contained As levels higher than the normal level in unexposed individuals (1 mg·kg−1). Most of the population (83.3%) showed As urinary levels exceeding the normal (<50 ng·mg−1). These results indicate the possibility of arsenicosis symptoms in residents of the areas studied. Among the three biomarkers tested, hair has shown to be a reliable indicator of metal exposures. The levels of As (r2 = 0.633), Ba (r2 = 0.646), Fe (r2 = 0.595), and Mo (r2 = 0.555) in hair were strongly positively associated with the levels of those metals in groundwater. In addition, significant weak correlations (p < 0.01) were found between levels of exposure to As and As concentrations in both nails (r2 = 0.544) and urine (r2 = 0.243).
Penradee Chanpiwat; Seiichiro Himeno; Suthipong Sthiannopkao. Arsenic and Other Metals’ Presence in Biomarkers of Cambodians in Arsenic Contaminated Areas. International Journal of Environmental Research and Public Health 2015, 12, 14285 -14300.
AMA StylePenradee Chanpiwat, Seiichiro Himeno, Suthipong Sthiannopkao. Arsenic and Other Metals’ Presence in Biomarkers of Cambodians in Arsenic Contaminated Areas. International Journal of Environmental Research and Public Health. 2015; 12 (11):14285-14300.
Chicago/Turabian StylePenradee Chanpiwat; Seiichiro Himeno; Suthipong Sthiannopkao. 2015. "Arsenic and Other Metals’ Presence in Biomarkers of Cambodians in Arsenic Contaminated Areas." International Journal of Environmental Research and Public Health 12, no. 11: 14285-14300.