Despite the importance of dairy farming in Uruguay, little information on dairy systems in this country is available in the scientific literature, and management practices that influence calf welfare at the herd level have not been explored. The aims of this study were to (1) describe the prepartum and calf-rearing systems, as well as the management practices that may influence calf welfare in pastured dairy herds in Uruguay, (2) estimate the annual calf mortality risk from birth to weaning, and (3) identify the primary clinical disease syndromes shown by the calves before death. A survey comprising a farm visit and a questionnaire was conducted on 225 randomly selected dairies with >30 milking cows, in 3 strata (31-99, 100-299, and ≥300 milking cows) of 6 Uruguayan departments where dairies are concentrated. Retrospective information from July 2013 to June 2014 was collected. A descriptive analysis was performed and results were inferred into the national dairy cattle population. Several management practices that could contribute to poor calf welfare were identified in a large proportion of farms. The annual calf mortality risk (calves that died between birth and weaning/calves born death or alive × 100, n = 149 farms) was 15.2%. Age at weaning averaged 75 d. Farmers reported that the most common clinical syndromes were diarrhea and respiratory disease in 85.2% and 47.5% of the farms, respectively. There was no continuous veterinary advice in 61.3% of the farms, 20.0% lacked data records, 38.5% had poor drainage in the prepartum area with waterlogging after rainfall, 52.1% monitored the prepartum area ≤2 times per day during the calving season, 65.1% did not perform navel antisepsis on newborns, 62.3% separated the calves from their dams at >24 h postpartum, 95.2% did not have a colostrum management program, 72.4% did not rotate the calf-rearing areas, 59.0% did not disinfect the calf feeders, 85.7% did not have staff dedicated exclusively to calf rearing, and 39.8% did not separate sick from healthy calves. The average volume of milk or milk replacer offered per calf was 4.5 L/day. Several of the identified management practices that affect calf welfare in the prepartum and calf-rearing periods could explain the high mortality risk. An effort should be made to conduct extension work focusing on the dissemination of good management practices to improve calf welfare and reduce calf mortality in Uruguayan dairy farms.

Uruguay is one of the main exporters of beef and dairy products, and cattle production is one of the main economic sectors in this country. Rotavirus A (RVA) is the main pathogen associated with neonatal calf diarrhea (NCD), a syndrome that leads to significant economic losses to the livestock industry. The aims of this study are to determine the frequency of RVA infections, and to analyze the genetic diversity of RVA strains in calves in Uruguay. A total of 833 samples from dairy and beef calves were analyzed through RT-qPCR and sequencing. RVA was detected in 57.0% of the samples. The frequency of detection was significantly higher in dairy (59.5%) than beef (28.4%) calves (p < 0.001), while it did not differ significantly among calves born in herds that were vaccinated (64.0%) or not vaccinated (66.7%) against NCD. The frequency of RVA detection and the viral load were significantly higher in samples from diarrheic (72.1%, 7.99 log₁₀ genome copies/mL of feces) than non-diarrheic (59.9%, 7.35 log₁₀ genome copies/mL of feces) calves (p < 0.005 and p = 0.007, respectively). The observed G-types (VP7) were G6 (77.6%), G10 (20.7%), and G24 (1.7%), while the P-types were P[5] (28.4%), P[11] (70.7%), and P[33] (0.9%). The G-type and P-type combinations were G6P[11] (40.4%), G6P[5] (38.6%), G10P[11] (19.3%), and the uncommon genotype G24P[33] (1.8%). VP6 and NSP1-5 genotyping were performed to better characterize some strains. The phylogenetic analyses suggested interspecies transmission, including transmission between animals and humans.

Aim To analyse group A rotavirus (RVA) environmental contamination in waters used for calves' consumption and to assess viral viability in dairy farm water sources. Methods and Results We analysed 202 samples of water used for calves' consumption and RVA was detected by RT-qPCR in 35·1% (95% CI: 28·9–42·0%). A marked pattern of seasonality was observed with higher frequency of detection in colder than warmer months (P = 0·002). There was no association between viral load and season or between the number of milking cows in the herd and the detection of RVA in the farm. The viability of the RVA particles detected was confirmed by isolation of RVA in cell culture from 5 of 10 water samples. Furthermore, an RVA waterborne outbreak of neonatal calf diarrhoea was described. Conclusions We demonstrate that RVA is frequent in dairy farm waters, and that the virus is infectious and capable of generating a diarrhoea outbreak. Significance and Impact of the Study Neonatal diarrhoea syndrome leads to economic losses to the livestock industry worldwide. To determine transmission routes is essential to take action in this regard and reduce the impact that this syndrome has for the livestock production. The results obtained in this work alert the dairy industry and highlight that mitigation strategies are crucial to improve the microbiological quality of this water.

Viral infections affecting cattle lead to economic losses to the livestock industry worldwide, but little is known about the circulation, pathogenicity and genetic diversity of enteric bovine astrovirus (BoAstV) in America. The aim of this work was to describe the prevalence and genetic diversity of enteric BoAstV in dairy cattle in Uruguay. A total of 457 fecal and 43 intestinal contents from dairy calves were collected between July 2015 and May 2017 and tested by RT-PCR, followed by sequencing and phylogenetic analyses of the polymerase and capsid regions. Twenty-six percent (128/500) of the samples were positive. Three different species within the Mamastrovirus genus were identified, including Mamastrovirus 28, Mamastrovirus 33 (3 samples each) and an unclassified Mamastrovirus species (19 samples). The unclassified species was characterized as a novel Mamastrovirus species. BoAstV circulates in Uruguayan dairy cattle with a high genetic diversity. The eventual clinicopathological significance of enteric BoAstV infection in cattle needs further investigation.