Highly diverseBradyrhizobiumstrains nodulate genistoid legumes (brooms) in the Canary Islands, Morocco, Spain and the Americas. Phylogenetic analyses of ITS,atpD,glnIIandrecAsequences revealed that these isolates represent at least four distinct evolutionary lineages within the genus, namelyBradyrhizobium japonicumand three unnamed genospecies. DNA–DNA hybridization experiments confirmed that one of the latter represents a new taxonomic species for which the nameBradyrhizobium canarienseis proposed.B. canariensepopulations experience homologous recombination at housekeeping loci, but are sexually isolated from sympatricB. japonicumbv.genistearumstrains in soils of the Canary Islands.B. canariensestrains are highly acid-tolerant, nodulate diverse legumes in the tribes Genisteae and Loteae, but notGlycinespecies, whereas acid-sensitiveB. japonicumsoybean isolates such as USDA 6^Tand USDA 110 do not nodulate genistoid legumes. Based on host-range experiments and phylogenetic analyses of symbioticnifHandnodCsequences, the biovarietiesgenistearumandglycinearumfor the genistoid legume and soybean isolates, respectively, were proposed.B. canariensebv.genistearumstrains display an overlapped host range withB. japonicumbv.genistearumisolates, both sharing monophyleticnifHandnodCalleles, possibly due to the lateral transfer of a conjugative chromosomal symbiotic island across species.B. canarienseis the sister species ofB. japonicum, as inferred from a maximum-likelihoodBradyrhizobiumspecies phylogeny estimated from congruentglnII+recAsequence partitions, which resolves eight species clades. In addition to the currently described species, this phylogeny uncovered the novelBradyrhizobiumgenospecies alpha and beta and the photosynthetic strains as independent evolutionary lineages. The type strain forB. canarienseis BTA-1^T(=ATCC BAA-1002^T=LMG 22265^T=CFNE 1008^T).

In our search for quorum-sensing (QS) disrupting molecules, 75 fungal isolates were recovered from reef organisms (endophytes), saline lakes and mangrove rhizosphere. Their QS inhibitory activity was evaluated in Chromobacterium violaceum CVO26. Four strains of endophytic fungi stood out for their potent activity at concentrations from 500 to 50 μg mL⁻¹. The molecular characterization, based on the internal transcribed spacer (ITS) region sequences (ITS1, 5.8S and ITS2) between the rRNA of 18S and 28S, identified these strains as belonging to four genera: Sarocladium (LAEE06), Fusarium (LAEE13), Epicoccum (LAEE14), and Khuskia (LAEE21). Interestingly, three came from coral species and two of them came from the same organism, the coral Diploria strigosa. Metabolic profiles obtained by Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS) suggest that a combination of fungal secondary metabolites and fatty acids could be the responsible for the observed activities. The LC-HRMS analysis also revealed the presence of potentially new secondary metabolites. This is, to the best of our knowledge, the first report of QS inhibition by marine endophytic fungi.