In this study, trypsin (Enzyme Comission 3.4.21.4) was immobilized in a low cost, lignocellulosic support (corn cob powder—CCP) with the goal of obtaining peptides with bioactive potential from cheese whey. The pretreated support was activated with glyoxyl groups, glutaraldehyde and IDA-glyoxyl. The immobilization yields of the derivatives were higher than 83%, and the retention of catalytic activity was higher than 74%. The trypsin-glyoxyl-CCP derivative was thermally stable at 65 °C, a value that was 1090-fold higher than that obtained with the free enzyme. The trypsin-IDA-glyoxyl-CCP and trypsin-glutaraldehyde-CCP derivatives had thermal stabilities that were 883- and five-fold higher, respectively, then those obtained with the free enzyme. In the batch experiments, trypsin-IDA-glyoxyl-CCP retained 91% of its activity and had a degree of hydrolysis of 12.49%, while the values for trypsin-glyoxyl-CCP were 87% and 15.46%, respectively. The stabilized derivative trypsin-glyoxyl-CCP was also tested in an upflow packed-bed reactor. The hydrodynamic characterization of this reactor was a plug flow pattern, and the kinetics of this system provided a relative activity of 3.04 ± 0.01 U·g⁻¹ and an average degree of hydrolysis of 23%, which were suitable for the production of potentially bioactive peptides.

The authors would like to add the following sentence to the “Acknowledgments” section of their article [1]:

Os hábitos alimentares inadequados estão diretamente relacionados ao desenvolvimento de doenças crônicas, como o câncer, cuja incidência crescente é confirmada por levantamentos epidemiológicos nacionais. O câncer de mama, que afeta predominantemente mulheres acima dos 40 anos, exige estratégias de prevenção focadas na nutrição. Diante desse quadro, este trabalho teve como objetivo analisar os benefícios das isoflavonas da soja na prevenção do câncer de mama e elaborar um folder educativo para o público-alvo. Para elucidar os benefícios das isoflavonas e sua relação com a prevenção, foi realizada uma revisão narrativa da literatura a partir da busca de artigos nas bases de dados on-line Pubmed e Scielo. Os resultados obtidos mostram uma relação positiva entre o consumo de isoflavonas e a redução do risco da doença, desde que associado a estilos de vida saudáveis. Conclui-se que a soja é uma aliada na prevenção e que a comunicação efetiva desses dados, através do folder, é crucial para a conscientização. Entretanto, sugere-se a necessidade de estudos mais abrangentes com grupos controle para evidenciar os mecanismos de ação das isoflavonas no organismo humano.