In this study, an effective circulating system was developed to remove heavy metals in medical waste incineration (MWI) fly ash. MWI fly ash (MWIFA)-column experiments were performed to remove Cu, Pb, Zn, Cd and Ni from MWIFA using EDTA disodium (Na₂EDTA). Iron-column experiments were conducted to study the removal effect of zero-valent iron on the five heavy metals from washing wastewater. TCLP test method was employed to evaluate heavy metals toxicity of MWIFA residues generated after 0-0.2 mol/L Na₂EDTA solution treated. After washed by 0.2 mol/L Na₂EDTA solution, TCLP leaching values of Cu, Pb, Zn, Cd and Ni were the lowest and satisfied the standard (GB 5085.3–2007), and the leaching values were 58.4±2.0 mg/L, 2.81±0.14 mg/L, 64.3±4.0 mg/L, 0.156±0.005 mg/L, 0.381±0.006 mg/L. Concentrations of Cu, Pb, Zn, Cd and Ni in iron-column effluent were reduced by 99.7%, 91.6%, 91.6%, 75.4% and 75.7%, respectively. Na₂EDTA was recovered and recycled to the removal of heavy metals from MWIFA. Compared new Na₂EDTA solution with recycled Na₂EDTA solution, recycled Na₂EDTA and water could be reutilized to dispose MWIFA. The removal efficiencies of Cu, Pb, Zn, Cd and Ni by recycled 0.2 mol/L Na₂EDTA solution were 67.1%, 68.8%, 63.2%, 73.9%, 50.7%, respectively, the removal efficiencies that using recovered Na₂EDTA decreased by 2.6%, 3.9%, 3.3%, 4.2%, and 1.6%, respectively. Successive batch experiments were also conducted to evaluate industrialization potential and reusable times for recycled Na₂EDTA. After 4 recirculation cycles, extraction efficiencies of Pb and Cd (removal efficiency at different cycles divided by removal efficiency of new Na₂EDTA) declined towards 80%.