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Małgorzata Kasprowicz-Potocka (PhD, MSc, female) is Associated Professor at PULS. Małgorzata is nutritionist specializing in animal nutrition, especially pigs. She also is intersting in feed additives and protein alternatives in animal diets, feed chemistry and feed processing as extrusion, germination, fermentation, etc. She is an author or co-author of more than 30 peer reviewed papers (H-index: 6), reviews, and popular articles. She is involved in supervising PhD, MSc and BSc students. Matador has many years experience in realization of programmes and grants (Ministry Programmes, National Programmes, Biostrateg).
The aim of this study was to: (1) provide controlled fermentation of narrow-leafed lupine seeds; (2) monitor seed composition, and (3) determine the influence of fermentation on the performance, gut environment and physiology, and selected blood metabolic parameters, in young pigs. Firstly, the effect of 24 h lupine seed fermentation by bacteria and yeast on seed chemical composition was determined. It increased contents of crude protein, crude fiber and ash, but reduced nitrogen-free extractive levels. The amino acid profile of fermented lupine (FL) was similar to that of raw lupine (RL) seeds, whereas the contents of oligosaccharides and P-phytate decreased significantly, in contrast to alkaloids. In fermented feed, pH dropped from 5.5 to 3.9. In the 28-day experiment, 24 male pigs were divided into three groups. The control group was fed a soybean meal diet (SBM), whereas in the experimental diets, 50% of SBM protein was replaced by RL or FL. Afterwards, eight pigs from each group were euthanized and their digesta and blood samples were collected. The FL use did not affect pigs’ performance, nor their metabolic, microbial and most gastrointestinal tract parameters, but influenced crypt depth. Fermentation affected concentrations of short chain fatty acids and p-cresole in the proximal colon segment. In the small intestine, the levels of acetate and butyrate decreased, and, in the caecum, the propionate level decreased. Fermentation significantly lowered the pH of the middle colon digesta and ammonia contents compared to RL. A part of SBM may be successfully replaced by RL and FL in young pigs’ diets.
Anita Zaworska-Zakrzewska; Małgorzata Kasprowicz-Potocka; Robert Mikuła; Marcin Taciak; Ewa Pruszyńska-Oszmałek; Andrzej Frankiewicz. Growth Performance, Gut Environment and Physiology of the Gastrointestinal Tract in Weaned Piglets Fed a Diet Supplemented with Raw and Fermented Narrow-Leafed Lupine Seeds. Animals 2020, 10, 2084 .
AMA StyleAnita Zaworska-Zakrzewska, Małgorzata Kasprowicz-Potocka, Robert Mikuła, Marcin Taciak, Ewa Pruszyńska-Oszmałek, Andrzej Frankiewicz. Growth Performance, Gut Environment and Physiology of the Gastrointestinal Tract in Weaned Piglets Fed a Diet Supplemented with Raw and Fermented Narrow-Leafed Lupine Seeds. Animals. 2020; 10 (11):2084.
Chicago/Turabian StyleAnita Zaworska-Zakrzewska; Małgorzata Kasprowicz-Potocka; Robert Mikuła; Marcin Taciak; Ewa Pruszyńska-Oszmałek; Andrzej Frankiewicz. 2020. "Growth Performance, Gut Environment and Physiology of the Gastrointestinal Tract in Weaned Piglets Fed a Diet Supplemented with Raw and Fermented Narrow-Leafed Lupine Seeds." Animals 10, no. 11: 2084.
The aim of this study was to compare the chemical composition of European soy seeds. A mycological and toxigenic screening was carried out on 18 varieties of soy seeds harvested in Poland. Moreover, the level of soybean meal (SBM) substitution by raw soybean seeds was analysed in terms of its effect on young pigs’ performance (body weight gain, feed intake, feed utilisation) along with apparent total tract digestibility (ATTD) of dry matter and crude protein in the diets. In a 28-day trial, 48 male pigs were tested using a marker method with TiO2. In their diets, SBM was replaced by soy seeds in the amounts of 0%, 5%, 10%, 15%, 20% and 25%. In the last 3 days of the experiment, samples of excreta from each animal separately were collected three times per day. The chemical composition of soybean seeds differed in terms of their contents of crude protein, ether extract, neutral detergent fibre and raffinose family oligosaccharides, as well as the trypsin inhibitor activity. Seeds were also contaminated with fungi, yeast and mycotoxins, mainly zearalenone and deoxynivalenol. The ATTD of crude protein ranged from 70.6% to 77.6% and that of dry matter from 93.5% to 94.6%, with no differences between the groups being found (p > 0.05). Pigs’ performance parameters were reduced strongly with increasing amounts of raw seeds in the diets (p < 0.05). The results indicate that only a 5% addition of raw soy seeds in pigs‘ diet is recommended.
Anita Zaworska-Zakrzewska; Małgorzata Kasprowicz-Potocka; Magdalena Twarużek; Robert Kosicki; Jan Grajewski; Zuzanna Wiśniewska; Andrzej Rutkowski. A Comparison of the Composition and Contamination of Soybean Cultivated in Europe and Limitation of Raw Soy Seed Content in Weaned Pigs’ Diets. Animals 2020, 10, 1972 .
AMA StyleAnita Zaworska-Zakrzewska, Małgorzata Kasprowicz-Potocka, Magdalena Twarużek, Robert Kosicki, Jan Grajewski, Zuzanna Wiśniewska, Andrzej Rutkowski. A Comparison of the Composition and Contamination of Soybean Cultivated in Europe and Limitation of Raw Soy Seed Content in Weaned Pigs’ Diets. Animals. 2020; 10 (11):1972.
Chicago/Turabian StyleAnita Zaworska-Zakrzewska; Małgorzata Kasprowicz-Potocka; Magdalena Twarużek; Robert Kosicki; Jan Grajewski; Zuzanna Wiśniewska; Andrzej Rutkowski. 2020. "A Comparison of the Composition and Contamination of Soybean Cultivated in Europe and Limitation of Raw Soy Seed Content in Weaned Pigs’ Diets." Animals 10, no. 11: 1972.
The rearing of piglets is the most difficult period in the pigs’ production because of their poorly developed digestive system and the low activity of digestive enzymes. Problems in nutrition and stress cause some disorders in the functioning of the digestive system leading to diarrhea and the mortality of piglets. Starting in 2006 in the EU, a total ban on antibiotics in their use as growth promoters was introduced. Since then, new and safe feed additives have been sought in order to replace antibiotics. Organic and inorganic acids as well as their salts were recognized as effective and safe additives. Due to their properties, they can improve feed palatability and digestibility, reduce the buffer capacity of feed, impact the development and functioning of the pig’s digestive system and improve the health and growth parameters. However, the effectiveness of acids is related to their qualitative and quantitative share in the feed additive. In this review, some strategies for using organic acids, their mixtures and also some new multi-component products will be discussed.
Piotr Nowak; Anita Zaworska-Zakrzewska; Andrzej Frankiewicz; Małgorzata Kasprowicz-Potocka. The effects and mechanisms of acids on the health of piglets and weaners – a review. Annals of Animal Science 2020, -1, 1 .
AMA StylePiotr Nowak, Anita Zaworska-Zakrzewska, Andrzej Frankiewicz, Małgorzata Kasprowicz-Potocka. The effects and mechanisms of acids on the health of piglets and weaners – a review. Annals of Animal Science. 2020; -1 (ahead-of-p):1.
Chicago/Turabian StylePiotr Nowak; Anita Zaworska-Zakrzewska; Andrzej Frankiewicz; Małgorzata Kasprowicz-Potocka. 2020. "The effects and mechanisms of acids on the health of piglets and weaners – a review." Annals of Animal Science -1, no. ahead-of-p: 1.
Małgorzata Kasprowicz-Potocka; Anita Zaworska-Zakrzewska; Andrzej Rutkowski. Effect of Phytase on Digestibility and Performance of Growing and Finishing Pigs Fed Diets with Lupins and Rapeseed Meal. Journal of Agricultural Science and Technology A 2020, 10, 1 .
AMA StyleMałgorzata Kasprowicz-Potocka, Anita Zaworska-Zakrzewska, Andrzej Rutkowski. Effect of Phytase on Digestibility and Performance of Growing and Finishing Pigs Fed Diets with Lupins and Rapeseed Meal. Journal of Agricultural Science and Technology A. 2020; 10 (4):1.
Chicago/Turabian StyleMałgorzata Kasprowicz-Potocka; Anita Zaworska-Zakrzewska; Andrzej Rutkowski. 2020. "Effect of Phytase on Digestibility and Performance of Growing and Finishing Pigs Fed Diets with Lupins and Rapeseed Meal." Journal of Agricultural Science and Technology A 10, no. 4: 1.
The objective of the study was to determine the effect of replacing soybean meal with 5% of raw soybean seeds on the growth, feed consumption and utilization of growing pigs, also the carcass and pork quality. The growth experiment was conducted on 120 pigs of approx. 18.5 kg allocated to two dietary treatments. The animals from the control treatment (CON) were offered a diet with soybean meal, and the experimental group (EXP) was given 5% raw soybean seeds (NON-GMO, Augusta var.) instead of SBM. The experiment lasted 88 days. After the experiment, eight pigs from each group were euthanized and meat samples were collected. No diet effects on the animals’ performance and carcass quality were observed (P>0.05). The experimental diet affected (P<0.05) meat color, and also meat composition (higher water content and lower intramuscular fat content). The composition of fatty acids in the meat did not differ significantly, except for higher C16:1 content in the EXP group. The introduction of 5% raw soybean seeds into the diets did not impact animal performance, but it reduced some indices of the pork quality.
Anita Zaworska-Zakrzewska; Małgorzata Kasprowicz-Potocka; Zuzanna Wiśniewska; Andrzej Rutkowski; Marcin Hejdysz; Sebastian Kaczmarek; Piotr Nowak; Anna Zmudzińska; Mirosław Banaszak. The Chemical Composition of Domestic Soybean Seeds and the Effects of Partial Substitution of Soybean Meal by Raw Soybean Seeds in the Diet on Pigs’ Growth Performance and Pork Quality (M. Longissimus Lumborum). Annals of Animal Science 2020, 20, 521 -533.
AMA StyleAnita Zaworska-Zakrzewska, Małgorzata Kasprowicz-Potocka, Zuzanna Wiśniewska, Andrzej Rutkowski, Marcin Hejdysz, Sebastian Kaczmarek, Piotr Nowak, Anna Zmudzińska, Mirosław Banaszak. The Chemical Composition of Domestic Soybean Seeds and the Effects of Partial Substitution of Soybean Meal by Raw Soybean Seeds in the Diet on Pigs’ Growth Performance and Pork Quality (M. Longissimus Lumborum). Annals of Animal Science. 2020; 20 (2):521-533.
Chicago/Turabian StyleAnita Zaworska-Zakrzewska; Małgorzata Kasprowicz-Potocka; Zuzanna Wiśniewska; Andrzej Rutkowski; Marcin Hejdysz; Sebastian Kaczmarek; Piotr Nowak; Anna Zmudzińska; Mirosław Banaszak. 2020. "The Chemical Composition of Domestic Soybean Seeds and the Effects of Partial Substitution of Soybean Meal by Raw Soybean Seeds in the Diet on Pigs’ Growth Performance and Pork Quality (M. Longissimus Lumborum)." Annals of Animal Science 20, no. 2: 521-533.
The objective of this study was to assess the effect of novel appAT1 and appAT2 phytase inclusion at 250 phytase units (FTU)/kg on weaned piglet performance, the apparent total tract digestibility of P and Ca, and bone mineralization. Piglets (48 males) were randomly divided into four treatment groups: a positive control (PC), with recommended levels of phosphorus (P) and calcium (Ca), a negative control (NC) deficient in P and Ca, and two experimental groups with NC diets supplemented with phytase derived from the appA gene of Escherichia coli. Diets fed in a mashed form were divided into prestarter (0–21 days) and starter (22–42 days) periods. During the whole period of the study, the experimental diets improved (p < 0.05) the body weight gain (BWG) and feed conversion ratio (FCR) compared to the NC diet. The apparent total tract digestibility (ATTD) of the dry matter and crude protein was not significantly different among the diets. Phytase-supplemented diets improved the ATTD of P (p < 0.05) and the ATTD of Ca (p < 0.05). Bone ash content in the third metacarpal and P and Ca content were improved among the phytase supplemented diets compared to the NC diet.
Zuzanna Wiśniewska; Lode Nollet; Anouk Lanckriet; Erik Vanderbeke; Spas Petkov; Nikolay Outchkourov; Małgorzata Kasprowicz-Potocka; Anita Zaworska-Zakrzewska; Sebastian A. Kaczmarek. Effect of Phytase Derived from the E. coli AppA Gene on Weaned Piglet Performance, Apparent Total Tract Digestibility and Bone Mineralization. Animals 2020, 10, 121 .
AMA StyleZuzanna Wiśniewska, Lode Nollet, Anouk Lanckriet, Erik Vanderbeke, Spas Petkov, Nikolay Outchkourov, Małgorzata Kasprowicz-Potocka, Anita Zaworska-Zakrzewska, Sebastian A. Kaczmarek. Effect of Phytase Derived from the E. coli AppA Gene on Weaned Piglet Performance, Apparent Total Tract Digestibility and Bone Mineralization. Animals. 2020; 10 (1):121.
Chicago/Turabian StyleZuzanna Wiśniewska; Lode Nollet; Anouk Lanckriet; Erik Vanderbeke; Spas Petkov; Nikolay Outchkourov; Małgorzata Kasprowicz-Potocka; Anita Zaworska-Zakrzewska; Sebastian A. Kaczmarek. 2020. "Effect of Phytase Derived from the E. coli AppA Gene on Weaned Piglet Performance, Apparent Total Tract Digestibility and Bone Mineralization." Animals 10, no. 1: 121.
The aim of the research was to determine the affectivity of 4 different eubiotic preparations on the growth performance of pigs, microbial status, the integrity of intestinal mucosa, and some blood parameters. The experiment was conducted for 28 days on 48 male piglets allocated to six dietary treatments. Group 1 was offered a diet without eubiotic; 2 – a diet with acids mixture; 3 – phytobiotic, medium-chain fatty acids (MCFA) and yeast; 4 – probiotic, MCFA, and yeast; 5 – phytobiotic, probiotic, acids mixture, and sodium butyrate; 6 – phytobiotic, probiotic, MCFA, and sodium butyrate. The average daily weight gains and feed intake were recorded. Blood samples, digesta samples, and ileal tissue samples were collected for studies. There was no significant difference in gain, feed intake, or FCR among the treatments as well as in the ileal and caecal pH value, microbial content, and total SCFA content in caecal digesta. Ammonia content in ileal digesta was significantly higher in comparison with other groups and in caecal digesta was significantly higher in group 6 in comparison with groups 1 and 2. Villi height was significantly higher (P<0.05) in groups 2, 3 and 6 compared to the control. Villi height to crypt depth ratio was significantly higher (P<0.05) in groups 5 and 2, but the most promising effects seem to be from combinations 3 and 4. In comparison with control: in groups 2, 3 and 5 higher ALT, glucose and triglyceride; in groups 3, 4 and 5 higher total protein and cholesterol; in group 4 higher albumin and in group 6 higher BUN, were found. Generally, used eubiotic preparations affected gut morphology and some blood parameters but did not affect microbiota, pig growth or feed utilization.
Piotr Nowak; Małgorzata Kasprowicz-Potocka; Anita Zaworska-Zakrzewska; Włodzimierz Nowak; Barbara Stefańska; Anna Sip; Włodzimierz Grajek; Katarzyna Grajek; Andrzej Frankiewicz. The Effect of Combined Feed Additives on Growing Pigs’ Performance and Digestive Tract Parameters. Annals of Animal Science 2019, 19, 807 -819.
AMA StylePiotr Nowak, Małgorzata Kasprowicz-Potocka, Anita Zaworska-Zakrzewska, Włodzimierz Nowak, Barbara Stefańska, Anna Sip, Włodzimierz Grajek, Katarzyna Grajek, Andrzej Frankiewicz. The Effect of Combined Feed Additives on Growing Pigs’ Performance and Digestive Tract Parameters. Annals of Animal Science. 2019; 19 (3):807-819.
Chicago/Turabian StylePiotr Nowak; Małgorzata Kasprowicz-Potocka; Anita Zaworska-Zakrzewska; Włodzimierz Nowak; Barbara Stefańska; Anna Sip; Włodzimierz Grajek; Katarzyna Grajek; Andrzej Frankiewicz. 2019. "The Effect of Combined Feed Additives on Growing Pigs’ Performance and Digestive Tract Parameters." Annals of Animal Science 19, no. 3: 807-819.
Anita Zaworska-Zakrzewska; Małgorzata Kasprowicz-Potocka; Zuzanna Wiśniewska Piotr Nowak; Andrzej Rutkowski. The Nutritional Value of Yellow Lupine (Lupinus luteus) for Growing Pigs. Journal of Agricultural Science and Technology A 2019, 9, 1 .
AMA StyleAnita Zaworska-Zakrzewska, Małgorzata Kasprowicz-Potocka, Zuzanna Wiśniewska Piotr Nowak, Andrzej Rutkowski. The Nutritional Value of Yellow Lupine (Lupinus luteus) for Growing Pigs. Journal of Agricultural Science and Technology A. 2019; 9 (6):1.
Chicago/Turabian StyleAnita Zaworska-Zakrzewska; Małgorzata Kasprowicz-Potocka; Zuzanna Wiśniewska Piotr Nowak; Andrzej Rutkowski. 2019. "The Nutritional Value of Yellow Lupine (Lupinus luteus) for Growing Pigs." Journal of Agricultural Science and Technology A 9, no. 6: 1.
Barbara Stefańska; Jolanta Komisarek; Daniel Stanisławski; Michał Gąsiorek; Małgorzata Kasprowicz-Potocka; Andrzej Frankiewicz; Włodzimierz Nowak. The effect of Yarrowia lipolytica culture on growth performance, ruminal fermentation and blood parameters of dairy calves. Animal Feed Science and Technology 2018, 243, 72 -79.
AMA StyleBarbara Stefańska, Jolanta Komisarek, Daniel Stanisławski, Michał Gąsiorek, Małgorzata Kasprowicz-Potocka, Andrzej Frankiewicz, Włodzimierz Nowak. The effect of Yarrowia lipolytica culture on growth performance, ruminal fermentation and blood parameters of dairy calves. Animal Feed Science and Technology. 2018; 243 ():72-79.
Chicago/Turabian StyleBarbara Stefańska; Jolanta Komisarek; Daniel Stanisławski; Michał Gąsiorek; Małgorzata Kasprowicz-Potocka; Andrzej Frankiewicz; Włodzimierz Nowak. 2018. "The effect of Yarrowia lipolytica culture on growth performance, ruminal fermentation and blood parameters of dairy calves." Animal Feed Science and Technology 243, no. : 72-79.
A. Zaworska; M. Kasprowicz-Potocka; A. Rutkowski; D. Jamroz. The influence of dietary raw and extruded field peas (Pisum sativum L.) on nutrients digestibility and performance of weaned and fattening pigs. Journal of Animal and Feed Sciences 2018, 27, 123 -130.
AMA StyleA. Zaworska, M. Kasprowicz-Potocka, A. Rutkowski, D. Jamroz. The influence of dietary raw and extruded field peas (Pisum sativum L.) on nutrients digestibility and performance of weaned and fattening pigs. Journal of Animal and Feed Sciences. 2018; 27 (2):123-130.
Chicago/Turabian StyleA. Zaworska; M. Kasprowicz-Potocka; A. Rutkowski; D. Jamroz. 2018. "The influence of dietary raw and extruded field peas (Pisum sativum L.) on nutrients digestibility and performance of weaned and fattening pigs." Journal of Animal and Feed Sciences 27, no. 2: 123-130.
M. Hejdysz; S. A. Kaczmarek; Marta Kubiś; D. Jamroz; M. Kasprowicz-Potocka; A. Zaworska; A. Rutkowski. Effect of increasing levels of raw and extruded narrow-leafed lupin seeds in broiler diet on performance parameters, nutrient digestibility and AMEN value of diet. Journal of Animal and Feed Sciences 2018, 1 .
AMA StyleM. Hejdysz, S. A. Kaczmarek, Marta Kubiś, D. Jamroz, M. Kasprowicz-Potocka, A. Zaworska, A. Rutkowski. Effect of increasing levels of raw and extruded narrow-leafed lupin seeds in broiler diet on performance parameters, nutrient digestibility and AMEN value of diet. Journal of Animal and Feed Sciences. 2018; ():1.
Chicago/Turabian StyleM. Hejdysz; S. A. Kaczmarek; Marta Kubiś; D. Jamroz; M. Kasprowicz-Potocka; A. Zaworska; A. Rutkowski. 2018. "Effect of increasing levels of raw and extruded narrow-leafed lupin seeds in broiler diet on performance parameters, nutrient digestibility and AMEN value of diet." Journal of Animal and Feed Sciences , no. : 1.
The aim of this study was to compare the effect of probiotic bacteria, prebiotics, phytobiotics and their combinations on performance and microbial activity in the digestive tract of growing pigs. The experiment was conducted over 28 d on 48 male pigs of about 12 kg body weight (BW), which were allocated to following treatments.: (1) Control Group (Con) without additive, (2) Group I, addition of a prebiotic (inulin), (3) Group Ph, a phytobiotic (herbal water extracts), (4) Group P, a probiotic composed of four strains of lactic acid bacteria, (5) Group PhP, phytobiotic and probiotic bacteria and (6) Group PhPI, a phytobiotic, probiotic bacteria and a prebiotic. Animal performance was recorded and at d 28 six pigs from each group were euthanised to collect digesta samples. In all groups except for Group I, diarrhoea incidents were observed. Groups Ph and P had significantly higher daily gains and final BW, and Group Ph utilised feed better than other groups. The pH of ileal digesta was significantly lower in Group PhPI. In the caecal digesta of Groups I, P and PhP, the pH level was lower than in the other groups but dry matter contents was significantly higher in Groups Con and I. The short-chain fatty acids and particular acid content differed significantly only in the colonic digesta. The yeast and mould numbers in caecal digesta was highest in Group Con. No treatment effects were observed for the number of lactic acid bacteria, coli group bacteria or Clostridium. However, the observed significantly higher number of total bacteria suggests that a multi-component eubiotic treatment changes the bacterial composition and distribution more effectively. Our findings indicated that all used additives changed the intestinal microflora, but the multi-component eubiotics were not beneficial as feed additives offered separately. Moreover, supplementation of phytobiotics and probiotic bacteria also improved the animal performance significantly.
Piotr Nowak; Małgorzata Kasprowicz-Potocka; Anita Zaworska; Włodzimierz Nowak; Barbara Stefańska; Anna Sip; Włodzimierz Grajek; Wojciech Juzwa; Marcin Taciak; Marcin Barszcz; Anna Tuśnio; Katarzyna Grajek; Joanna Foksowicz-Flaczyk; Andrzej Frankiewicz. The effect of eubiotic feed additives on the performance of growing pigs and the activity of intestinal microflora. Archives of Animal Nutrition 2017, 71, 455 -469.
AMA StylePiotr Nowak, Małgorzata Kasprowicz-Potocka, Anita Zaworska, Włodzimierz Nowak, Barbara Stefańska, Anna Sip, Włodzimierz Grajek, Wojciech Juzwa, Marcin Taciak, Marcin Barszcz, Anna Tuśnio, Katarzyna Grajek, Joanna Foksowicz-Flaczyk, Andrzej Frankiewicz. The effect of eubiotic feed additives on the performance of growing pigs and the activity of intestinal microflora. Archives of Animal Nutrition. 2017; 71 (6):455-469.
Chicago/Turabian StylePiotr Nowak; Małgorzata Kasprowicz-Potocka; Anita Zaworska; Włodzimierz Nowak; Barbara Stefańska; Anna Sip; Włodzimierz Grajek; Wojciech Juzwa; Marcin Taciak; Marcin Barszcz; Anna Tuśnio; Katarzyna Grajek; Joanna Foksowicz-Flaczyk; Andrzej Frankiewicz. 2017. "The effect of eubiotic feed additives on the performance of growing pigs and the activity of intestinal microflora." Archives of Animal Nutrition 71, no. 6: 455-469.
The usefulness of Candida utilis, Saccharomyces cerevisiae, and Kluyveromyces lactis to the fermentation of high alkaloid lupin seeds was examined. Seeds were mixed with water and sterilized at 121 °C for 20 min. After cooling, the mixture was inoculated with yeast and fermented for 48 or 72 hr at 30 °C. After enzymes deactivation samples were dried. Fermentation increased the content of crude protein (p < .05) and affected the amino acid profile of the protein (p < .05). There was no yeast strain effect on the crude protein content, but the true protein content was higher (p > .05) after K. lactis and S. cerevisieae fermentation. After 72 hr fermentation the content of crude protein was significantly higher but several necessary amino acids content was lower in comparison with 48 hr fermentation time. Fermentation reduced (p < .05) phytate, raffinose family oligosaccharide content and also alkaloid content in the final products. Fermentation process increased total and lactic acid bacteria count, the number of yeasts and molds as well as the number of coliform bacteria. The three yeast strains used were not fully suitable for the fermentation of high alkaloid lupin seeds. Candida utilis strain was found as the most favorable because of better chemical and microbial characteristic of fermented products. The present study reports, that the fermentation process increased protein content and partially improved quality microbial and antinutritional character of seeds by reducing raffinose family oligosaccharides, phytate content, and alkaloids Taking this into account, fermented lupin seeds could be recommended as an alternative, protein-rich component of diets for animals and people. However, it should be remembered that lupin is a potential allergen and its status after fermentation has not yet been investigated.
Małgorzata Kasprowicz-Potocka; A. Zaworska; Piotr Gulewicz; P. Nowak; A. Frankiewicz. The effect of fermentation of high alkaloid seeds of Lupinus angustifolius var. Karo by Saccharomyces cerevisieae , Kluyveromyces lactis , and Candida utilis on the chemical and microbial composition of products. Journal of Food Processing and Preservation 2017, 42, e13487 .
AMA StyleMałgorzata Kasprowicz-Potocka, A. Zaworska, Piotr Gulewicz, P. Nowak, A. Frankiewicz. The effect of fermentation of high alkaloid seeds of Lupinus angustifolius var. Karo by Saccharomyces cerevisieae , Kluyveromyces lactis , and Candida utilis on the chemical and microbial composition of products. Journal of Food Processing and Preservation. 2017; 42 (2):e13487.
Chicago/Turabian StyleMałgorzata Kasprowicz-Potocka; A. Zaworska; Piotr Gulewicz; P. Nowak; A. Frankiewicz. 2017. "The effect of fermentation of high alkaloid seeds of Lupinus angustifolius var. Karo by Saccharomyces cerevisieae , Kluyveromyces lactis , and Candida utilis on the chemical and microbial composition of products." Journal of Food Processing and Preservation 42, no. 2: e13487.
The aims of this study were to provide a controlled fermentation process of blue lupin seeds (Lupinus angustifolius, cv. Neptun), monitor the changes in seed composition and determine the influence of the fermentation on the apparent ileal digestibility (AID) of crude protein (CP) and amino acids in growing pigs, compared with raw lupin seeds. The fermentation with bacteria and yeast was conducted for 24 h at 25ºC under aerobic conditions. Seed fermentation increased the contents of CP, fibre, fat and ash and most of the analysed amino acids but reduced the levels of the nitrogen-free extractives. Furthermore, fermentation decreased the contents of raffinose family oligosaccharides and phytic acids but increased the alkaloid content. The AID was estimated on three barrows (mean initial body weight 25 kg), surgically fitted with a T-cannula in the distal ileum. The pigs received three diets, each for 6 d, within three experimental periods (3 × 3 Latin Square design). The diets contained soybean meal (Group SBM), raw lupin seeds (Group RL) or fermented lupin seeds (Group FL) as solely protein sources. Fermentation had a positive impact on the AID of CP and methionine, cysteine, isoleucine, leucine, phenylalanine and valine (p < 0.05). Feeding raw or fermented lupin seeds did not affect the microbial status of the ileal digesta. Moreover, ammonia content in the caecal digesta did not differ between Groups RL and FL, although it was significantly higher than in Group SBM. It can be concluded that the fermentation process modified the chemical composition of nutrients in seeds, which can influence the digestibility and utilisation of the fermentation product in animal diets compared to raw seeds.
Anita Zaworska; Andrzej Frankiewicz; Małgorzata Kasprowicz-Potocka. The influence of narrow-leafed lupin seed fermentation on their chemical composition and ileal digestibility and microbiota in growing pigs. Archives of Animal Nutrition 2017, 71, 285 -296.
AMA StyleAnita Zaworska, Andrzej Frankiewicz, Małgorzata Kasprowicz-Potocka. The influence of narrow-leafed lupin seed fermentation on their chemical composition and ileal digestibility and microbiota in growing pigs. Archives of Animal Nutrition. 2017; 71 (4):285-296.
Chicago/Turabian StyleAnita Zaworska; Andrzej Frankiewicz; Małgorzata Kasprowicz-Potocka. 2017. "The influence of narrow-leafed lupin seed fermentation on their chemical composition and ileal digestibility and microbiota in growing pigs." Archives of Animal Nutrition 71, no. 4: 285-296.
A. Zaworska; M. Kasprowicz-Potocka; A. Frankiewicz; Zenon Zdunczyk; Jerzy Juskiewicz. Effects of fermentation of narrow-leafed lupine (L. angustifolius) seeds on their chemical composition and physiological parameters in rats. Journal of Animal and Feed Sciences 2016, 25, 326 -334.
AMA StyleA. Zaworska, M. Kasprowicz-Potocka, A. Frankiewicz, Zenon Zdunczyk, Jerzy Juskiewicz. Effects of fermentation of narrow-leafed lupine (L. angustifolius) seeds on their chemical composition and physiological parameters in rats. Journal of Animal and Feed Sciences. 2016; 25 (4):326-334.
Chicago/Turabian StyleA. Zaworska; M. Kasprowicz-Potocka; A. Frankiewicz; Zenon Zdunczyk; Jerzy Juskiewicz. 2016. "Effects of fermentation of narrow-leafed lupine (L. angustifolius) seeds on their chemical composition and physiological parameters in rats." Journal of Animal and Feed Sciences 25, no. 4: 326-334.
S.A. Kaczmarek; M. Hejdysz; Marta Kubiś; M. Kasprowicz-Potocka; A. Rutkowski. The nutritional value of yellow lupin (Lupinus luteus L.) for broilers. Animal Feed Science and Technology 2016, 222, 43 -53.
AMA StyleS.A. Kaczmarek, M. Hejdysz, Marta Kubiś, M. Kasprowicz-Potocka, A. Rutkowski. The nutritional value of yellow lupin (Lupinus luteus L.) for broilers. Animal Feed Science and Technology. 2016; 222 ():43-53.
Chicago/Turabian StyleS.A. Kaczmarek; M. Hejdysz; Marta Kubiś; M. Kasprowicz-Potocka; A. Rutkowski. 2016. "The nutritional value of yellow lupin (Lupinus luteus L.) for broilers." Animal Feed Science and Technology 222, no. : 43-53.
The effect of soya bean meal (SBM) substitution in the diet with white lupine (WL) seeds on performances and some parameters of the digestive tract of pigs were studied. The digestibility experiment was performed on 30 pigs using difference method combined with marker method, where one group was offered basal diet and in diet of experimental groups 25% of the basal diet was substituted with SBM or WL meal. The growth experiment was conducted on 48 pigs of approximately 20 kg body weight. The animals from the control group were fed with diet containing SBM, whereas in the experimental groups 50% or 100% of SBM was substituted with WL seeds. At the end of the experiment, eight pigs from each group were euthanized, and digesta and tissues were sampled. The apparent total tract digestibility of crude protein and dry matter was higher (p < 0.05), but crude fibre and ether extract were lower (p < 0.05) in the WL seeds than in SBM. Substituting 50% of SBM in the control diet with WL seeds did not affect significantly pig performance. Total substitution of SBM with WL seeds impacted negatively pigs growth and feed intake (p < 0.05), but it did not affect the length of villi and crypt depth. It also significantly increased ileum digesta viscosity and ammonia concentration in the ileum and caecum digesta. In the ileal digesta of animals offered WL seeds, an increased (p < 0.05) total bacterial and Enterobacteriaceae numbers were found, whereas reduced number of yeast and mould was found in the caecal digesta. The substitution of 50% SBM with WL seeds in the growing pig diet did not affect pigs' results, but the total substitution significantly decreased the performance of pigs and affected intestinal ecology.
M. Kasprowicz-Potocka; A. Zaworska; S. Kaczmarek; M. Hejdysz; R. Mikuła; A. Rutkowski. The effect ofLupinus albusseeds on digestibility, performance and gastrointestinal tract indices in pigs. Journal of Animal Physiology and Animal Nutrition 2016, 101, e216 -e224.
AMA StyleM. Kasprowicz-Potocka, A. Zaworska, S. Kaczmarek, M. Hejdysz, R. Mikuła, A. Rutkowski. The effect ofLupinus albusseeds on digestibility, performance and gastrointestinal tract indices in pigs. Journal of Animal Physiology and Animal Nutrition. 2016; 101 (5):e216-e224.
Chicago/Turabian StyleM. Kasprowicz-Potocka; A. Zaworska; S. Kaczmarek; M. Hejdysz; R. Mikuła; A. Rutkowski. 2016. "The effect ofLupinus albusseeds on digestibility, performance and gastrointestinal tract indices in pigs." Journal of Animal Physiology and Animal Nutrition 101, no. 5: e216-e224.
The aim of this study was to determine the nutrient digestibility of seeds of four varieties of narrow-leafed lupines (Lupinus angustifolius) and the possibility of soya bean meal (SBM) substitution by lupine seeds alone and in combination with rapeseed meal (RSM) in the diets of pigs. The seeds of the lupine varieties Kalif, Sonet, Zeus and Boruta were analysed. The apparent total tract digestibility (ATTD) was determined on 50 cross-bred pigs using the difference method with titanium dioxide as a marker. The substitution of SBM by lupine seeds alone (at 0 - 100%) was tested on 60 pigs (20-105 kg body weight (BW)) and by a combination of lupine seeds and RSM on 180 fattening pigs (35-80 kg BW). The chemical composition of lupine seeds differed considerably, especially in terms of crude protein and mineral content. All seeds contained less than 0.05% alkaloids and 9.3% oligosaccharides in dry matter. The ATTD of protein ranged from 70% to 74%, those of ether extract from 36% to 55% and those of gross energy from 77% to 84%. The entire replacement of SBM by lupine seeds (var. Sonet) did not have a negative effect on the performance of grower and fattener pigs. The substitution of SBM by a combination of lupines and RSM reduced the performance of growing and finishing pigs significantly.
Małgorzata Kasprowicz-Potocka; Anita Zaworska-Zakrzewska; Sebastian Kaczmarek; Andrzej Rutkowski. The nutritional value of narrow-leafed lupine (Lupinus angustifolius) for fattening pigs. Archives of Animal Nutrition 2016, 70, 209 -223.
AMA StyleMałgorzata Kasprowicz-Potocka, Anita Zaworska-Zakrzewska, Sebastian Kaczmarek, Andrzej Rutkowski. The nutritional value of narrow-leafed lupine (Lupinus angustifolius) for fattening pigs. Archives of Animal Nutrition. 2016; 70 (3):209-223.
Chicago/Turabian StyleMałgorzata Kasprowicz-Potocka; Anita Zaworska-Zakrzewska; Sebastian Kaczmarek; Andrzej Rutkowski. 2016. "The nutritional value of narrow-leafed lupine (Lupinus angustifolius) for fattening pigs." Archives of Animal Nutrition 70, no. 3: 209-223.
The aim of the study was to investigate the effect of BCS (Body Condition Score) on the calving day and its decrease during early lactation on the biochemical blood indices and reproductive performance. One hundred and thirty-one Polish Holstein-Friesian cows were divided into three groups according to the North-American body condition score on the calving day (AC≤3.5 point BCS; MID 3.51-3.75 point BCS; FAT>3.75 point BCS) and according to the decrease in BCS during early lactation (HG >0.49 point BCS; WEL 0.49-0.25 point BCS, L3.75 BCS) the lowest conception rate of the first insemination, insemination index and thereby the longest days open were observed. The highest decreases in BCS (HG >0.49 points) resulted in increased concentrations of NEFA on 3 d and of BHBA on 3 and 5 d. Moreover, in the HG group, the lowest concentration of T3 on 3, 5 and 28 d as compared to the WEL group was recorded. We concluded that the BCS (>3.5 points) on the calving day had a significantly negative effect on the metabolic status of dairy cows in the postpartum period estimated by the concentration of biochemical blood indices characterising carbohydrates (IGF-I, insulin) and lipid (NEFA, BHBA) metabolism and also the reproductive performance such as the conception rate of the first insemination, insemination index, days open. We suggested that the blood serum concentrations of IGF-I and NEFA were the most sensitive biochemical markers of the metabolic status of dairy cows in our study.
Barbara Stefańska; Włodzimierz Nowak; Ewa Pruszynska-Oszmalek; Robert Mikuła; Daniel Stanisławski; Małgorzata Kasprowicz-Potocka; Andrzej Frankiewicz; Paweł Maćkowiak. The Effect of Body Condition Score on the Biochemical Blood Indices and Reproductive Performance of Dairy Cows. Annals of Animal Science 2016, 16, 129 -143.
AMA StyleBarbara Stefańska, Włodzimierz Nowak, Ewa Pruszynska-Oszmalek, Robert Mikuła, Daniel Stanisławski, Małgorzata Kasprowicz-Potocka, Andrzej Frankiewicz, Paweł Maćkowiak. The Effect of Body Condition Score on the Biochemical Blood Indices and Reproductive Performance of Dairy Cows. Annals of Animal Science. 2016; 16 (1):129-143.
Chicago/Turabian StyleBarbara Stefańska; Włodzimierz Nowak; Ewa Pruszynska-Oszmalek; Robert Mikuła; Daniel Stanisławski; Małgorzata Kasprowicz-Potocka; Andrzej Frankiewicz; Paweł Maćkowiak. 2016. "The Effect of Body Condition Score on the Biochemical Blood Indices and Reproductive Performance of Dairy Cows." Annals of Animal Science 16, no. 1: 129-143.
The effect of 24-hour fermentation of lupin seeds by different yeast strains on their chemical composition was determined. After fermentation, the mass fraction of proteins increased and their in vitro digestibility and biological activity significantly improved. The amino acid profile of fermented products was similar to that of raw lupin seeds. The significant reduction in the mass fraction of oligosaccharides and phytate, but not of alkaloids was found. The pH level of fermented products decreased as a consequence of the increase of lactic and propionic acid mass fractions. The most favourable changes in the chemical composition of blue lupin seeds were obtained in fermentation with Saccharomyces cerevisiae baker’s yeast and Fermivin 7013 strain.
Małgorzata Kasprowicz-Potocka; Paulina Borowczyk; Anita Zaworska; Włodzimierz Nowak; Andrzej Frankiewicz; Piotr Gulewicz. The Effect of Dry Yeast Fermentation on Chemical Composition and Protein Characteristics of Blue Lupin Seeds. Food Technology and Biotechnology 2016, 54, 360 -366.
AMA StyleMałgorzata Kasprowicz-Potocka, Paulina Borowczyk, Anita Zaworska, Włodzimierz Nowak, Andrzej Frankiewicz, Piotr Gulewicz. The Effect of Dry Yeast Fermentation on Chemical Composition and Protein Characteristics of Blue Lupin Seeds. Food Technology and Biotechnology. 2016; 54 (3):360-366.
Chicago/Turabian StyleMałgorzata Kasprowicz-Potocka; Paulina Borowczyk; Anita Zaworska; Włodzimierz Nowak; Andrzej Frankiewicz; Piotr Gulewicz. 2016. "The Effect of Dry Yeast Fermentation on Chemical Composition and Protein Characteristics of Blue Lupin Seeds." Food Technology and Biotechnology 54, no. 3: 360-366.