Hepatitis C virus caused acute and chronic hepatitis. Micro RNA could be used as disease biomarkers. This study was look into Micro RNA125b, Micro RNA 489-3p, and biochemical analysis as a diagnostic for HCV. This study included 20 individuals had chronic liver disease admitted to the Gastroenterology Hospital in Mansoura and Oncology Institute in Mansoura. All individuals were subjected to full clinical examinations. Following analyzing all patients, polymerase chain reaction (PCR) was confirmed to be positive for chronic HCV in 5 individuals. Five negative control samples were taken from healthy control group at the same age range and sex. Serum liver function parameters and AFP with gene expression of microRNA125b and microRNA 489-3p were estimated.. Results: The HCV group significantly increased AST, ALT, DBIL, TBIL, ALP, GGT, and ALB) while significantly decreasing albumin concentrations. In the HCV group AFP was substantially higher than in control. The HCV group had a considerable rise in miRNA125b (10.07+ 0.19) but a significant drop in miRNA489-3p (0.36+ 0.02). In addition to biochemical investigation, we found that miRNA125b and miRNA489-3p expression levels employed as valuable marker for HCV diagnosis. Keywords: MicroRNA 125b , MicroRNA 489-3p, HCV.

Hepatitis B virus (HBV) is a blood-borne virus that poses chief public health threats and it is the frequent reason for cirrhosis all over the world. microRNA could be used as disease biomarkers. This investigation was carried out to investigate mRNA -125b, mRNA 489-3p and biochemical analysis as marker for chronic HBV. This work included 20 patients had chronic liver disease admitted to the Gastroenterology Hospital and Oncology Institute in Mansoura. After investigating the patients, 5 patients, from the 20 patients, were positive PCR chronic HBV. Serum samples were obtained from those 5 patients. For control, negative serum samples were collected at the same age range and sex. Liver function parameters and AFP were estimated. RNA was extracted from serum for gene expression of mRNA125b and mRNA 489-3p. The main results were; HBV group showed a significant increase in ALT, AST, TBIL, GGT, ALP while there was significant decrease in albumin levels. In HBV group AFP was significantly increased compared to control. HBV group revealed a significant increase (6.70 ± 0.18) in mRNA125b, with a significant decrease in mRNA489-3p (0.51 ± 0.03). On conclusion, mRNA125b and mRNA489-3p expressions along with biochemical analysis could be used as an accurate marker for HBV diagnosis. Keywords: microRNA 125b , microRNA 489-3p, HBV.