One of the main threats to the survival of Asian turtles is the demand in China for turtles for use as food and medicine products. As the demand for turtle products escalated over the past 20 years entrepreneurs initiated commercial breeding facilities for profit. To gain a better understanding of the scale of the captive turtle trade we conducted a survey of the 1,499 large turtle farms known by branch offices of the Endangered Species Import and Export Management Office and the Provincial Forestry Bureaus. The results from the 684 respondent farms (46%) indicated that a total of >300 million turtles are sold per year and are worth c. USD 750 million. Although the bulk of these figures comprise the common Chinese softshell turtle Pelodiscus sinensis many other species are also farmed, including Critically Endangered species and even species native to North America. As 54% of known farms did not respond to our survey we suggest that the trade in captive turtles is probably a multi-billion dollar industry. This is likely considering that many Chinese turtle farms operate covertly and are thus impossible to survey. The large scale of turtle farming in China raises questions about the impact of so many threatened turtles being bred and sold for profit. Because the commercial breeding of these species is so well developed and large in scale, the deleterious impact is serious and difficult to control.

China is the largest consumer of turtles in the world and international trade has been cited as the greatest threat to Asian turtles. Two main types of trade in live turtles occur in China: for food and traditional Chinese medicine, and for pets, including those for release by Buddhists. The food trade involves the largest quantities of turtles. In recent years, however, the international pet turtle trade has increased dramatically. Yuehe Pet Market in Guangzhou is the largest pet market in China. selling live chelonians and other animals. To understand the potential impacts of the pet trade on chelonians we conducted seven surveys in Yuehe Pet Market from August 2006 to March 2008. Over 39,000 individual chelonians of 61 species were recorded (19.1% of the global total of 319 species). Fifteen (24.6%) of these species are native to China and 46 (75.4%) are native to other countries. Two are designated as grade II key state-protected species in China. Thirty-eight (62.3%) are CITES listed species (four in CITES Appendix I, 26 in CITES II and eight in CITES III). Four are categorized on the IUCN Red List as Critically Endangered, 16 as Endangered and 19 as Vulnerable. Our surveys indicate that increasing demand and the illegal international pet trade could be having a severe impact on chelonian conservation, and we make recommendations for law enforcement and conservation.

Over the past 16 years, 13 new species of geoemydid turtles have been described from China. Ten of these new species are based on specimens purchased through the Hong Kong animal trade. Unfortunately, attempts by scientists to discover wild populations of some these newly described species have failed, raising questions about the legitimacy of the type localities and concerns over the validity of the species. Here the phylogenetic and taxonomic validity of two of these species is tested. Mitochondrial DNA haplotypes and allozyme genotypes of specimens matching the descriptions of Mauremys iversoni and Cuora serrata are compared to specimens of established species collected from known localities. The available evidence is consistent with the hypothesis that the specimens represent polyphyletic, intergeneric hybrids. The systematic status of all the new forms of turtles described from pet trade specimens are critical data for conservation efforts, particularly captive breeding.

Ammonia is one of major pollutants in aquatic environment that induces severe stress and toxicity to organisms in aquatic system. The intestine acts a major defense line that protects living organisms from biotic and abiotic stresses. In the current study, we examined the effects of ammonia on intestinal histomorphology, transcriptional levels of intestinal barrier functioning genes and intestinal microbiota of Chinese striped-neck turtle (Mauremys sinensis). Thus, the turtles were placed in water with addition of ammonia at 0 (control), 100, 200 mg L^-1 for 30 days. Our findings showed that ammonia reduced the villus length and induced the inflammatory cells appearance. In addition, the epithelial tight junction genes, claudin and zonola occludin significantly downregulated in ammonia exposed groups as compared to control group (P < 0.05). Similarly, the mRNA expression levels of MUC-2 gene also significantly decreased in ammonia treated groups (P < 0.05). However, the expression levels of intestinal immune related genes such as IL-10, IL-12, TGF-β1, TNF-α and IFN-γ significantly increased (P < 0.05). Furthermore, ammonia changed gut microbial diversity variedly. At the phylum levels, Firmicutes increased, whereas Bacteroidota, Desulfobacterota and Synergistota decreased significantly. Likewise, Lachnospiraceae, Bacteroides, Eubacteriaceae, Desulfovibrio, Muribaculaceae, Bilophila, Cloacibacillus, Christensenellaceae, Ruminococcus and Parabacteroides decreased while, Romboutsia and Turicibacter increased in ammonia exposed groups. In conclusion, ammonia at 100 and 200 mg L^-1 could alter the intestinal barrier function and change the composition of intestinal microbiota, leading to bad health status in M. sinensis.