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Eutrophication of Lake Victoria led to changes in its phytoplankton communities. However, different levels of eutrophication exist in the open lake and the bays, and between embayments. This study utilized spatial and temporal sampling of Napoleon Gulf and Murchison Bay, exhibiting different trophic conditions. Over one year, we investigated phytoplankton biomass, richness, diversity and dissimilarity, and related the dynamics of the dominant species to the limnological and climatic conditions. The results confirmed that Napoleon Gulf and Murchison Bay showed large differences in eutrophication status, with lower nutrient concentrations in Napoleon Gulf than in Murchison Bay, where a strong gradient was observed from inshore to offshore areas. These nutrient dynamics resulted in a 4 to 10 fold higher chlorophyll-a in Murchison Bay than in Napoleon Gulf. From the embayments, 135 phytoplankton taxa were recorded with no significant differences in alpha diversity. However, high dissimilarity in community structure was observed in beta diversity, mostly due to a turnover among the dominant toxigenic species. Thus, from a similar species pool, there was a shift in the dominant toxigenic cyanobacteria from Microcystis flos-aquae and M. aeruginosa in Murchison Bay, Dolichospermum circinale and Planktolyngbya circumcreta in Napoleon Gulf to D. circinale in the offshore stations. These cyanobacteria are toxigenic taxa with known health hazards. Using partial least square models, we showed that both climatic variables (e.g. wind, solar radiation) and levels of inorganic dissolved nutrients (e.g. SRP, NO3–, and NH4+) are the main drivers of differences and dominance in cyanobacteria communities in northern Lake Victoria.
Mark Olokotum; Marc Troussellier; Arthur Escalas; Sahima Hamlaoui; William Okello; Ronald Semyalo; Jean-François Humbert; Cécile Bernard. High nutrient loading and climatic parameters influence the dominance and dissimilarity of toxigenic cyanobacteria in northern bays of Lake Victoria. Journal of Great Lakes Research 2021, 47, 985 -996.
AMA StyleMark Olokotum, Marc Troussellier, Arthur Escalas, Sahima Hamlaoui, William Okello, Ronald Semyalo, Jean-François Humbert, Cécile Bernard. High nutrient loading and climatic parameters influence the dominance and dissimilarity of toxigenic cyanobacteria in northern bays of Lake Victoria. Journal of Great Lakes Research. 2021; 47 (4):985-996.
Chicago/Turabian StyleMark Olokotum; Marc Troussellier; Arthur Escalas; Sahima Hamlaoui; William Okello; Ronald Semyalo; Jean-François Humbert; Cécile Bernard. 2021. "High nutrient loading and climatic parameters influence the dominance and dissimilarity of toxigenic cyanobacteria in northern bays of Lake Victoria." Journal of Great Lakes Research 47, no. 4: 985-996.
The supply of drinking water is a vital challenge for the people who live on the African continent, as this continent is experiencing strong demographic growth and therefore increasing water demands. To meet these needs, surface water resources are becoming increasingly mobilized because underground resources are not always available or have already been overexploited. This situation is the case in the region of Abidjan in the Ivory Coast, where the drinking water deficit is a growing problem and it is therefore necessary to mobilize new water resources to ensure the supply of drinking water. Among the potential resources, local managers have identified a freshwater lagoon, Lagoon Aghien, That is in close proximity to the city of Abidjan. With the aim of enhancing knowledge on the ecological functioning of the lagoon and contributing to the assessment of its ability to provide drinking water, several physical and chemical parameters of the water and the phytoplankton community of the lagoon were monitored for 17 months (December 2016-April 2018) at six sampling stations. Our findings show that the lagoon is eutrophic, as evidenced by the high concentrations of total phosphorus (>140 μg L-1), nitrogen (1.36 mg L-1) and average chlorophyll-a (26 to 167 μg L-1) concentrations. The phytoplankton community in the lagoon is dominated by genera typical of eutrophic environments including mixotrophic genera such as Peridinium and by cyanobacteria such as Cylindrospermopsis/Raphidiopsis, Microcystis and Dolichospermum that can potentially produce cyanotoxins. The two rainfall peaks that occur in June and October appeared to be major events in terms of nutrient flows entering the lagoon, and the dynamics of these flows are complex. Significant differences were also found in the nutrient concentrations and to a lesser extent in the phytoplankton communities among the different stations, especially during the rainfall peaks. Overall, these results reveal that the quality of the lagoon’s water is already severely degraded, and this degradation could increase in future years due to increasing urbanization in the watershed. These results therefore raise questions about the potential use of the lagoon as a source of drinking water if measures are not taken very quickly to protect this lagoon from increasing eutrophication and other pollution sources.
Mathias Koffi Ahoutou; Rosine Yao Djeha; Eric Kouamé Yao; Catherine Quiblier; Julie Niamen-Ebrottié; Sahima Hamlaoui; Kevin Tambosco; Jean-Louis Perrin; Marc Troussellier; Cécile Bernard; Luc Seguis; Marc Bouvy; Jacques Pédron; Felix Koffi Konan; Jean-François Humbert; Julien Kalpy Coulibaly. Assessment of some key indicators of the ecological status of an African freshwater lagoon (Lagoon Aghien, Ivory Coast). PLOS ONE 2021, 16, e0251065 .
AMA StyleMathias Koffi Ahoutou, Rosine Yao Djeha, Eric Kouamé Yao, Catherine Quiblier, Julie Niamen-Ebrottié, Sahima Hamlaoui, Kevin Tambosco, Jean-Louis Perrin, Marc Troussellier, Cécile Bernard, Luc Seguis, Marc Bouvy, Jacques Pédron, Felix Koffi Konan, Jean-François Humbert, Julien Kalpy Coulibaly. Assessment of some key indicators of the ecological status of an African freshwater lagoon (Lagoon Aghien, Ivory Coast). PLOS ONE. 2021; 16 (5):e0251065.
Chicago/Turabian StyleMathias Koffi Ahoutou; Rosine Yao Djeha; Eric Kouamé Yao; Catherine Quiblier; Julie Niamen-Ebrottié; Sahima Hamlaoui; Kevin Tambosco; Jean-Louis Perrin; Marc Troussellier; Cécile Bernard; Luc Seguis; Marc Bouvy; Jacques Pédron; Felix Koffi Konan; Jean-François Humbert; Julien Kalpy Coulibaly. 2021. "Assessment of some key indicators of the ecological status of an African freshwater lagoon (Lagoon Aghien, Ivory Coast)." PLOS ONE 16, no. 5: e0251065.
Cyanobacteria are able to synthesize a high diversity of natural compounds that account for their success in the colonization of a variety of ecological niches. Many of them have beneficial properties. The mud from the thermal baths of Balaruc-Les-Bains, one of the oldest thermal baths in France, has long been recognized as a healing treatment for arthro-rheumatic diseases. To characterize the cyanobacteria living in these muds, several strains were isolated from the water column and biofilms of the retention basin and analyzed using a polyphasic approach. Morphological, ultrastructural and molecular (16S rRNA gene and 16S-23S ITS region sequencing) methods were employed to identify nine cyanobacterial strains belonging to the orders Chroococcales, Synechococcales, Oscillatoriales and Nostocales. The combination of morphological and genetic characteristics supported the description of a new genus and species with the type species as Pseudochroococcus coutei. The taxonomic diversity in the muds from Thermes de Balaruc-Les-Bains appears higher than previously documented, providing new candidate taxa for their observed therapeutic properties.
C Duval; S Hamlaoui; B Piquet; G Toutirais; C Yéprémian; A Reinhardt; S Duperron; B Marie; J Demay; C Bernard. Diversity of cyanobacteria from thermal muds (Balaruc-Les-Bains, France) with the description of Pseudochroococcus coutei gen. nov., sp. nov. FEMS Microbes 2021, 2, 1 .
AMA StyleC Duval, S Hamlaoui, B Piquet, G Toutirais, C Yéprémian, A Reinhardt, S Duperron, B Marie, J Demay, C Bernard. Diversity of cyanobacteria from thermal muds (Balaruc-Les-Bains, France) with the description of Pseudochroococcus coutei gen. nov., sp. nov. FEMS Microbes. 2021; 2 ():1.
Chicago/Turabian StyleC Duval; S Hamlaoui; B Piquet; G Toutirais; C Yéprémian; A Reinhardt; S Duperron; B Marie; J Demay; C Bernard. 2021. "Diversity of cyanobacteria from thermal muds (Balaruc-Les-Bains, France) with the description of Pseudochroococcus coutei gen. nov., sp. nov." FEMS Microbes 2, no. : 1.
Background: The Balaruc-les-Bains’ thermal mud was found to be colonized predominantly by microorganisms, with cyanobacteria constituting the primary organism in the microbial biofilm observed on the mud surface. The success of cyanobacteria in colonizing this specific ecological niche can be explained in part by their taxa-specific adaptation capacities, and also the diversity of bioactive natural products that they synthesize. This array of components has physiological and ecological properties that may be exploited for various applications. Methods: Nine cyanobacterial strains were isolated from Balaruc thermal mud and maintained in the Paris Museum Collection (PMC). Full genome sequencing was performed coupled with targeted and untargeted metabolomic analyses (HPLC-DAD and LC-MS/MS). Bioassays were performed to determine antioxidant, anti-inflammatory, and wound-healing properties. Results: Biosynthetic pathways for phycobiliproteins, scytonemin, and carotenoid pigments and 124 metabolite biosynthetic gene clusters (BGCs) were characterized. Several compounds with known antioxidant or anti-inflammatory properties, such as carotenoids, phycobilins, mycosporine-like amino acids, and aeruginosins, and other bioactive metabolites like microginins, microviridins, and anabaenolysins were identified. Secretion of the proinflammatory cytokines TNF-α, IL-1β, IL-6, and IL-8 appeared to be inhibited by crude extracts of Planktothricoides raciborskii PMC 877.14, Nostoc sp. PMC 881.14, and Pseudo-chroococcus couteii PMC 885.14. The extract of the Aliinostoc sp. PMC 882.14 strain was able to slightly enhance migration of HaCat cells that may be helpful in wound healing. Several antioxidant compounds were detected, but no significant effects on nitric oxide secretion were observed. There was no cytotoxicity on the three cell types tested, indicating that cyanobacterial extracts may have anti-inflammatory therapeutic potential without harming body cells. These data open up promising uses for these extracts and their respective molecules in drugs or thermal therapies.
Justine Demay; Sébastien Halary; Adeline Knittel-Obrecht; Pascal Villa; Charlotte Duval; Sahima Hamlaoui; Théotime Roussel; Claude Yéprémian; Anita Reinhardt; Cécile Bernard; Benjamin Marie. Anti-Inflammatory, Antioxidant, and Wound-Healing Properties of Cyanobacteria from Thermal Mud of Balaruc-Les-Bains, France: A Multi-Approach Study. Biomolecules 2020, 11, 28 .
AMA StyleJustine Demay, Sébastien Halary, Adeline Knittel-Obrecht, Pascal Villa, Charlotte Duval, Sahima Hamlaoui, Théotime Roussel, Claude Yéprémian, Anita Reinhardt, Cécile Bernard, Benjamin Marie. Anti-Inflammatory, Antioxidant, and Wound-Healing Properties of Cyanobacteria from Thermal Mud of Balaruc-Les-Bains, France: A Multi-Approach Study. Biomolecules. 2020; 11 (1):28.
Chicago/Turabian StyleJustine Demay; Sébastien Halary; Adeline Knittel-Obrecht; Pascal Villa; Charlotte Duval; Sahima Hamlaoui; Théotime Roussel; Claude Yéprémian; Anita Reinhardt; Cécile Bernard; Benjamin Marie. 2020. "Anti-Inflammatory, Antioxidant, and Wound-Healing Properties of Cyanobacteria from Thermal Mud of Balaruc-Les-Bains, France: A Multi-Approach Study." Biomolecules 11, no. 1: 28.
Cyanobacteria are able to synthesize a high diversity of natural compounds that account for their success in the colonization of a variety of ecological niches. Many of them have beneficial properties. The mud from the thermal baths of Balaruc-Les-Bains, one of the oldest thermal baths in France, has long been recognized as a healing treatment for arthro-rheumatic diseases. To characterize the cyanobacteria living in these muds and the metabolites they potentially produce, several strains were isolated from the water column and biofilms of the retention basin and analyzed using a polyphasic approach. Morphological, ultrastructural and molecular (16S rRNA gene and 16S-23S ITS region sequencing) methods were employed to identify nine cyanobacterial strains belonging to the orders Chroococcales, Synechococcales, Oscillatoriales and Nostocales. The combination of morphological and genetic characteristics supported the description of a new genus and species with the type species as Pseudo-chroococcus couteii. The high taxonomic diversity in the muds of the thermal baths of Balaruc-Les-Bains along with literature reports of the potential for bioactive metabolite synthesis of these taxa allowed us to hypothesize that some of the metabolites produced by these strains could contribute to the therapeutic properties of the muds from Thermes de Balaruc-Les-Bains.
Charlotte Duval; Sahima Hamlaoui; Berenice Piquet; Geraldine Toutirais; Claude Yepremian; A. Reinhardt; Sebastien Duperron; Benjamin Marie; Justine DeMay; Cecile Bernard. Characterization of cyanobacteria isolated from thermal muds of Balaruc-Les-Bains (France) and description of a new genus and species Pseudo-chroococcus couteii. 2020, 1 .
AMA StyleCharlotte Duval, Sahima Hamlaoui, Berenice Piquet, Geraldine Toutirais, Claude Yepremian, A. Reinhardt, Sebastien Duperron, Benjamin Marie, Justine DeMay, Cecile Bernard. Characterization of cyanobacteria isolated from thermal muds of Balaruc-Les-Bains (France) and description of a new genus and species Pseudo-chroococcus couteii. . 2020; ():1.
Chicago/Turabian StyleCharlotte Duval; Sahima Hamlaoui; Berenice Piquet; Geraldine Toutirais; Claude Yepremian; A. Reinhardt; Sebastien Duperron; Benjamin Marie; Justine DeMay; Cecile Bernard. 2020. "Characterization of cyanobacteria isolated from thermal muds of Balaruc-Les-Bains (France) and description of a new genus and species Pseudo-chroococcus couteii." , no. : 1.
Background: The Balaruc-les-Bains’ thermal mud was found to be colonized predominantly by microorganisms, with cyanobacteria constituting the primary organism in the microbial biofilm observed on the mud surface. The success of cyanobacteria in colonizing this specific ecological niche can be explained in part by their taxa-specific adaptation capacities, and also the diversity of bioactive natural products that they synthesize. This array of components has physiological and ecological properties that may be exploited for various applications.
Justine DeMay; Sébastien Halary; Adeline Knittel-Obrecht; Pascal Villa; Charlotte Duval; Sahima Hamlaoui; Théotime Roussel; Claude Yéprémain; Anita Reinhardt; Cécile Bernard; Benjamin Marie. Anti-Inflammatory, Antioxidant and Wound Healing Properties of Cyanobacteria from Thermal Mud of Balaruc-les-Bains, France: A Multi-Approach Study. 2020, 1 .
AMA StyleJustine DeMay, Sébastien Halary, Adeline Knittel-Obrecht, Pascal Villa, Charlotte Duval, Sahima Hamlaoui, Théotime Roussel, Claude Yéprémain, Anita Reinhardt, Cécile Bernard, Benjamin Marie. Anti-Inflammatory, Antioxidant and Wound Healing Properties of Cyanobacteria from Thermal Mud of Balaruc-les-Bains, France: A Multi-Approach Study. . 2020; ():1.
Chicago/Turabian StyleJustine DeMay; Sébastien Halary; Adeline Knittel-Obrecht; Pascal Villa; Charlotte Duval; Sahima Hamlaoui; Théotime Roussel; Claude Yéprémain; Anita Reinhardt; Cécile Bernard; Benjamin Marie. 2020. "Anti-Inflammatory, Antioxidant and Wound Healing Properties of Cyanobacteria from Thermal Mud of Balaruc-les-Bains, France: A Multi-Approach Study." , no. : 1.
Benthic cyanobacteria strains from Guadeloupe have been investigated for the first time by combining phylogenetic, chemical and biological studies in order to better understand the taxonomic and chemical diversity as well as the biological activities of these cyanobacteria through the effect of their specialized metabolites. Therefore, in addition to the construction of the phylogenetic tree, indicating the presence of 12 potentially new species, an LC-MS/MS data analysis workflow was applied to provide an overview on chemical diversity of 20 cyanobacterial extracts, which was linked to antimicrobial activities evaluation against human pathogenic and ichtyopathogenic environmental strains.
Sébastien Duperron; Mehdi A. Beniddir; Sylvain Durand; Arlette Longeon; Charlotte Duval; Olivier Gros; Cécile Bernard; Marie-Lise Bourguet-Kondracki. New Benthic Cyanobacteria from Guadeloupe Mangroves as Producers of Antimicrobials. Marine Drugs 2019, 18, 16 .
AMA StyleSébastien Duperron, Mehdi A. Beniddir, Sylvain Durand, Arlette Longeon, Charlotte Duval, Olivier Gros, Cécile Bernard, Marie-Lise Bourguet-Kondracki. New Benthic Cyanobacteria from Guadeloupe Mangroves as Producers of Antimicrobials. Marine Drugs. 2019; 18 (1):16.
Chicago/Turabian StyleSébastien Duperron; Mehdi A. Beniddir; Sylvain Durand; Arlette Longeon; Charlotte Duval; Olivier Gros; Cécile Bernard; Marie-Lise Bourguet-Kondracki. 2019. "New Benthic Cyanobacteria from Guadeloupe Mangroves as Producers of Antimicrobials." Marine Drugs 18, no. 1: 16.
Evaluating the causes and consequences of dominance by a limited number of taxa in phytoplankton communities is of huge importance in the current context of increasing anthropogenic pressures on natural ecosystems. This is of particular concern in densely populated urban areas where usages and impacts of human populations on water ecosystems are strongly interconnected. Microbial biodiversity is commonly used as a bioindicator of environmental quality and ecosystem functioning, but there are few studies at the regional scale that integrate the drivers of dominance in phytoplankton communities and their consequences on the structure and functioning of these communities. Here, we studied the causes and consequences of phytoplankton dominance in 50 environmentally contrasted waterbodies, sampled over four summer campaigns in the highly-populated Île-de-France region (IDF). Phytoplankton dominance was observed in 32-52% of the communities and most cases were attributed to Chlorophyta (35.5-40.6% of cases) and Cyanobacteria (30.3-36.5%). The best predictors of dominance were identified using multinomial logistic regression and included waterbody features (surface, depth and connection to the hydrological network) and water column characteristics (total N, TN:TP ratio, water temperature and stratification). The consequences of dominance were dependent on the identity of the dominant organisms and included modifications of biological attributes (richness, cohesion) and functioning (biomass, RUE) of phytoplankton communities. We constructed co-occurrence networks using high resolution phytoplankton biomass and demonstrated that networks under dominance by Chlorophyta and Cyanobacteria exhibited significantly different structure compared with networks without dominance. Furthermore, dominance by Cyanobacteria was associated with more profound network modifications (e.g. cohesion, size, density, efficiency and proportion of negative links), suggesting a stronger disruption of the structure and functioning of phytoplankton communities in the conditions in which this group dominates. Finally, we provide a synthesis on the relationships between environmental drivers, dominance status, community attributes and network structure.
Arthur Escalas; Arnaud Catherine; Selma Maloufi; Maria Cellamare; Sahima Hamlaoui; Claude Yéprémian; Clarisse Louvard; Marc Troussellier; Cécile Bernard. Drivers and ecological consequences of dominance in periurban phytoplankton communities using networks approaches. Water Research 2019, 163, 114893 .
AMA StyleArthur Escalas, Arnaud Catherine, Selma Maloufi, Maria Cellamare, Sahima Hamlaoui, Claude Yéprémian, Clarisse Louvard, Marc Troussellier, Cécile Bernard. Drivers and ecological consequences of dominance in periurban phytoplankton communities using networks approaches. Water Research. 2019; 163 ():114893.
Chicago/Turabian StyleArthur Escalas; Arnaud Catherine; Selma Maloufi; Maria Cellamare; Sahima Hamlaoui; Claude Yéprémian; Clarisse Louvard; Marc Troussellier; Cécile Bernard. 2019. "Drivers and ecological consequences of dominance in periurban phytoplankton communities using networks approaches." Water Research 163, no. : 114893.
Efficient RNA extraction methods are needed to study transcript regulation. Such methods must lyse the cell without degrading the genetic material. For cyanobacteria this can be particularly challenging because of the presence of the cyanobacteria cell envelope. The great breath of cyanobacterial shape and size (unicellular, colonial, or filamentous multicellular) created a variety of cell lysis methods. However, there is still a lack of reliable techniques for nucleic acid extraction for several types of cyanobacteria. Here we designed and tested 15 extraction methods using physical, thermic or chemical stress on the filamentous cyanobacteria Planktothrix agardhii. Techniques based on the use of beads, sonication, and heat shock appeared to be too soft to break the Planktothrix agardhii cell envelope, whereas techniques based on the use of detergents degraded the cell envelope but also the RNA. Two protocols allowed to successfully obtain good-quality RNA. The first protocol consisted to manually crush the frozen cell pellet with a pestle and the second was based on the use of high-intensity ultra-sonication. When comparing these two, the high-intensity ultra-sonication protocol was less laborious, faster and allowed to extract 3.5 times more RNA compared to the liquid nitrogen pestle protocol. The high-intensity ultra-sonication protocol was then tested on five Planktothrix strains, this protocol allowed to obtain >8.5 μg of RNA for approximatively 3.5 × 108 cells. The extracted RNA were characterized by 260/280 and 260/230 ratio > to 2, indicating that the samples were devoid of contaminant, and RNA Quality Number > to 7, meaning that the integrity of RNA was preserved with this extraction method. In conclusion, the method we developed based on high-intensity ultra-sonication proved its efficacy in the extraction of Planktothrix RNA and could be helpful for other types of samples.
Sandra Kim Tiam; Katia Comte; Caroline Dalle; Charlotte Duval; Claire Pancrace; Muriel Gugger; Benjamin Marie; Claude Yéprémian; Cécile Bernard. Development of a new extraction method based on high-intensity ultra-sonication to study RNA regulation of the filamentous cyanobacteria Planktothrix. PLOS ONE 2019, 14, e0222029 .
AMA StyleSandra Kim Tiam, Katia Comte, Caroline Dalle, Charlotte Duval, Claire Pancrace, Muriel Gugger, Benjamin Marie, Claude Yéprémian, Cécile Bernard. Development of a new extraction method based on high-intensity ultra-sonication to study RNA regulation of the filamentous cyanobacteria Planktothrix. PLOS ONE. 2019; 14 (9):e0222029.
Chicago/Turabian StyleSandra Kim Tiam; Katia Comte; Caroline Dalle; Charlotte Duval; Claire Pancrace; Muriel Gugger; Benjamin Marie; Claude Yéprémian; Cécile Bernard. 2019. "Development of a new extraction method based on high-intensity ultra-sonication to study RNA regulation of the filamentous cyanobacteria Planktothrix." PLOS ONE 14, no. 9: e0222029.
Cyanobacteria are an ancient lineage of slow-growing photosynthetic bacteria and a prolific source of natural products with diverse chemical structures and potent biological activities and toxicities. The chemical identification of these compounds remains a major bottleneck. Strategies that can prioritize the most prolific strains and novel compounds are of great interest. Here, we combine chemical analysis and genomics to investigate the chemodiversity of secondary metabolites based on their pattern of distribution within some cyanobacteria. Planktothrix being a cyanobacterial genus known to form blooms worldwide and to produce a broad spectrum of toxins and other bioactive compounds, we applied this combined approach on four closely related strains of Planktothrix. The chemical diversity of the metabolites produced by the four strains was evaluated using an untargeted metabolomics strategy with high-resolution LC–MS. Metabolite profiles were correlated with the potential of metabolite production identified by genomics for the different strains. Although, the Planktothrix strains present a global similarity in terms of a biosynthetic cluster gene for microcystin, aeruginosin, and prenylagaramide for example, we found remarkable strain-specific chemodiversity. Only few of the chemical features were common to the four studied strains. Additionally, the MS/MS data were analyzed using Global Natural Products Social Molecular Networking (GNPS) to identify molecular families of the same biosynthetic origin. In conclusion, we depict an efficient, integrative strategy for elucidating the chemical diversity of a given genus and link the data obtained from analytical chemistry to biosynthetic genes of cyanobacteria.
Sandra Kim Tiam; Muriel Gugger; Justine DeMay; Séverine Le Manach; Charlotte Duval; Cécile Bernard; Benjamin Marie. Insights into the Diversity of Secondary Metabolites of Planktothrix Using a Biphasic Approach Combining Global Genomics and Metabolomics. Toxins 2019, 11, 498 .
AMA StyleSandra Kim Tiam, Muriel Gugger, Justine DeMay, Séverine Le Manach, Charlotte Duval, Cécile Bernard, Benjamin Marie. Insights into the Diversity of Secondary Metabolites of Planktothrix Using a Biphasic Approach Combining Global Genomics and Metabolomics. Toxins. 2019; 11 (9):498.
Chicago/Turabian StyleSandra Kim Tiam; Muriel Gugger; Justine DeMay; Séverine Le Manach; Charlotte Duval; Cécile Bernard; Benjamin Marie. 2019. "Insights into the Diversity of Secondary Metabolites of Planktothrix Using a Biphasic Approach Combining Global Genomics and Metabolomics." Toxins 11, no. 9: 498.
Photosynthetic organisms need to sense and respond to fluctuating environmental conditions, to perform efficient photosynthesis and avoid the formation of harmful reactive oxygen species. Cyanobacteria have developed a photoprotective mechanism that decreases the energy arriving at the reaction centers by increasing thermal energy dissipation at the level of the phycobilisome, the extramembranal light-harvesting antenna. This mechanism is triggered by the photoactive orange carotenoid protein (OCP). In this study, we characterized OCP and the related photoprotective mechanism in non-stressed and light-stressed cells of three different strains of Planktothrix that can form impressive blooms. In addition to changing lake ecosystemic functions and biodiversity, Planktothrix blooms can have adverse effects on human and animal health as they produce toxins (e.g., microcystins). Three Planktothrix strains were selected: two green strains, PCC 10110 (microcystin producer) and PCC 7805 (non-microcystin producer), and one red strain, PCC 7821. The green strains colonize shallow lakes with higher light intensities while red strains proliferate in deep lakes. Our study allowed us to conclude that there is a correlation between the ecological niche in which these strains proliferate and the rates of induction and recovery of OCP-related photoprotection. However, differences in the resistance to prolonged high-light stress were correlated to a better replacement of damaged D1 protein and not to differences in OCP photoprotection. Finally, microcystins do not seem to be involved in photoprotection as was previously suggested.
Chakib Djediat; Kathleen Feilke; Arthur Brochard; Lucie Caramelle; Sandra Kim Tiam; Pierre Sétif; Theo Gauvrit; Claude Yéprémian; Adjélé Wilson; Léa Talbot; Benjamin Marie; Diana Kirilovsky; Cécile Bernard. Light stress in green and red Planktothrix strains: The orange carotenoid protein and its related photoprotective mechanism. Biochimica et Biophysica Acta (BBA) - Reviews on Cancer 2019, 1861, 148037 .
AMA StyleChakib Djediat, Kathleen Feilke, Arthur Brochard, Lucie Caramelle, Sandra Kim Tiam, Pierre Sétif, Theo Gauvrit, Claude Yéprémian, Adjélé Wilson, Léa Talbot, Benjamin Marie, Diana Kirilovsky, Cécile Bernard. Light stress in green and red Planktothrix strains: The orange carotenoid protein and its related photoprotective mechanism. Biochimica et Biophysica Acta (BBA) - Reviews on Cancer. 2019; 1861 (4):148037.
Chicago/Turabian StyleChakib Djediat; Kathleen Feilke; Arthur Brochard; Lucie Caramelle; Sandra Kim Tiam; Pierre Sétif; Theo Gauvrit; Claude Yéprémian; Adjélé Wilson; Léa Talbot; Benjamin Marie; Diana Kirilovsky; Cécile Bernard. 2019. "Light stress in green and red Planktothrix strains: The orange carotenoid protein and its related photoprotective mechanism." Biochimica et Biophysica Acta (BBA) - Reviews on Cancer 1861, no. 4: 148037.
Cyanobacteria are photosynthetic microorganisms that colonize diverse environments worldwide, ranging from ocean to freshwaters, soils, and extreme environments. Their adaptation capacities and the diversity of natural products that they synthesize, support cyanobacterial success in colonization of their respective ecological niches. Although cyanobacteria are well-known for their toxin production and their relative deleterious consequences, they also produce a large variety of molecules that exhibit beneficial properties with high potential in various fields (e.g., a synthetic analog of dolastatin 10 is used against Hodgkin’s lymphoma). The present review focuses on the beneficial activities of cyanobacterial molecules described so far. Based on an analysis of 670 papers, it appears that more than 90 genera of cyanobacteria have been observed to produce compounds with potentially beneficial activities in which most of them belong to the orders Oscillatoriales, Nostocales, Chroococcales, and Synechococcales. The rest of the cyanobacterial orders (i.e., Pleurocapsales, Chroococcidiopsales, and Gloeobacterales) remain poorly explored in terms of their molecular diversity and relative bioactivity. The diverse cyanobacterial metabolites possessing beneficial bioactivities belong to 10 different chemical classes (alkaloids, depsipeptides, lipopeptides, macrolides/lactones, peptides, terpenes, polysaccharides, lipids, polyketides, and others) that exhibit 14 major kinds of bioactivity. However, no direct relationship between the chemical class and the respective bioactivity of these molecules has been demonstrated. We further selected and specifically described 47 molecule families according to their respective bioactivities and their potential uses in pharmacology, cosmetology, agriculture, or other specific fields of interest. With this up-to-date review, we attempt to present new perspectives for the rational discovery of novel cyanobacterial metabolites with beneficial bioactivity.
Justine DeMay; Cécile Bernard; Anita Reinhardt; Benjamin Marie. Natural Products from Cyanobacteria: Focus on Beneficial Activities. Marine Drugs 2019, 17, 320 .
AMA StyleJustine DeMay, Cécile Bernard, Anita Reinhardt, Benjamin Marie. Natural Products from Cyanobacteria: Focus on Beneficial Activities. Marine Drugs. 2019; 17 (6):320.
Chicago/Turabian StyleJustine DeMay; Cécile Bernard; Anita Reinhardt; Benjamin Marie. 2019. "Natural Products from Cyanobacteria: Focus on Beneficial Activities." Marine Drugs 17, no. 6: 320.
The effect of toxin-producing cyanobacterial blooms on fishes health has been investigated extensively, but no study to date has evaluated this effect on fish-associated microbiota. In this study, we test the effect of pure microcystins and of crude extracts of metabolites from Microcystis aeruginosa cultures on the composition of gut bacterial microbiota in Medaka fishes (Oryzias latipes) exposed for 28 days in a microcosm experiment. A 16S rRNA-based marker gene-based approach was used to investigate the composition of bacterial communities. Results show that fish gut community compositions differ from those occurring in the water, and among individual fishes. Exposure to extracts, rather than pure microcystin, has a significant influence on gut community composition, with a marked increase in relative abundances of pathogen-related bacteria (genera Nocardia and Mycobacterium) in the presence of one extract, and of bacterial orders Sphingomonadales and Saprospirales in the other. We suggest that compounds identified in the cyanobacterial extracts, but not microcystin LR alone, alter the composition of bacterial communities, with possible consequences for various biological functions in fishes. This pioneer microcosm experiment indicates that cyanobacterial blooms probably have an effect on fish gut microbiota and associated functions, including toxin degradation and feed efficiency, and should be further explored.
Sébastien Duperron; Sébastien Halary; Myriam Habiballah; Alison Gallet; Hélène Huet; Charlotte Duval; Cécile Bernard; Benjamin Marie. Response of Fish Gut Microbiota to Toxin-Containing Cyanobacterial Extracts: A Microcosm Study on the Medaka (Oryzias latipes). Environmental Science & Technology Letters 2019, 6, 341 -347.
AMA StyleSébastien Duperron, Sébastien Halary, Myriam Habiballah, Alison Gallet, Hélène Huet, Charlotte Duval, Cécile Bernard, Benjamin Marie. Response of Fish Gut Microbiota to Toxin-Containing Cyanobacterial Extracts: A Microcosm Study on the Medaka (Oryzias latipes). Environmental Science & Technology Letters. 2019; 6 (6):341-347.
Chicago/Turabian StyleSébastien Duperron; Sébastien Halary; Myriam Habiballah; Alison Gallet; Hélène Huet; Charlotte Duval; Cécile Bernard; Benjamin Marie. 2019. "Response of Fish Gut Microbiota to Toxin-Containing Cyanobacterial Extracts: A Microcosm Study on the Medaka (Oryzias latipes)." Environmental Science & Technology Letters 6, no. 6: 341-347.
Cyanobacteria are photosynthetic microorganisms that colonize diverse environments worldwide, ranging from ocean to freshwaters, soils, and extreme environments. Their adaptation capacities and the diversity of natural products (molecules, metabolites, or compounds) that they synthesize support the cyanobacterial success for the colonization of their respective ecological niches. Although cyanobacteria are well-known for their toxin production and their relative deleterious consequences, they also produce a large variety of molecules that exhibit beneficial properties with high potential for various fields of application (e.g., synthetic analog of the dolastatin 10 used against Hodgkin lymphoma). The present review specially focuses on the beneficial activities of cyanobacterial molecules described so far. Based on an analysis of 670 papers, it appears that more than 90 genera of cyanobacteria have been found to produce compounds with potential beneficial activities, most of them belonging to the orders Oscillatoriales, Nostocales Chroococcales, and Synechococcales. The rest of the cyanobacterial orders (i.e., Pleurocapsales, Chroococcidiopsales, and Gloeobacterales) remain poorly explored in terms of their molecular diversity and relative bioactivity. The diverse cyanobacterial molecules presenting beneficial bioactivities belong to 10 different chemical classes (alkaloids, depsipeptides, lipopeptides, macrolides/lactones, peptides, terpenes, polysaccharides, lipids, polyketides, and others) that exhibit 14 major kinds of bioactivity. However, no direct relation between the chemical class and the bioactivity of these molecules has been demonstrated. We further selected and specifically described 50 molecule families according to their specific bioactivities and their potential uses in pharmacology, cosmetology, agriculture, or other specific fields of interest. This up-to-date review takes advantage of the recent progresses in genome sequencing and biosynthetic pathway elucidation, and presents new perspectives for the rational discovery of new cyanobacterial metabolites with beneficial bioactivity.
Justine DeMay; Cécile Bernard; Anita Reinhardt; Benjamin Marie. Natural Products from Cyanobacteria: Focus on Beneficial Activities. 2019, 1 .
AMA StyleJustine DeMay, Cécile Bernard, Anita Reinhardt, Benjamin Marie. Natural Products from Cyanobacteria: Focus on Beneficial Activities. . 2019; ():1.
Chicago/Turabian StyleJustine DeMay; Cécile Bernard; Anita Reinhardt; Benjamin Marie. 2019. "Natural Products from Cyanobacteria: Focus on Beneficial Activities." , no. : 1.
Cyanobacteria are photosynthetic prokaryotes capable of synthesizing a large variety of secondary metabolites that exhibit significant bioactivity or toxicity. Microcystis constitutes one of the most common cyanobacterial genera, forming the intensive blooms that nowadays arise in freshwater ecosystems worldwide. Species in this genus can produce numerous cyanotoxins (i.e., toxic cyanobacterial metabolites), which can be harmful to human health and aquatic organisms. To better understand variations in cyanotoxin production between clones of Microcystis species, we investigated the diversity of 24 strains isolated from the same blooms or from different populations in various geographical areas. Strains were compared by genotyping with 16S-ITS fragment sequencing and metabolite chemotyping using LC ESI-qTOF mass spectrometry. While genotyping can help to discriminate among different species, the global metabolome analysis revealed clearly discriminating molecular profiles among strains. These profiles could be clustered primarily according to their global metabolite content, then according to their genotype, and finally according to their sampling location. A global molecular network of all metabolites produced by Microcystis species highlights the production of a wide set of chemically diverse metabolites, including a few microcystins, many aeruginosins, microginins, cyanopeptolins, and anabaenopeptins, together with a large set of unknown molecules. These components, which constitute the molecular biodiversity of Microcystis species, still need to be investigated in terms of their structure and potential bioactivites (e.g., toxicity).
Séverine Le Manach; Charlotte Duval; Arul Marie; Chakib Djediat; Arnaud Catherine; Marc Edery; Cecile Bernard; Benjamin Marie. Global Metabolomic Characterizations of Microcystis spp. Highlights Clonal Diversity in Natural Bloom-Forming Populations and Expands Metabolite Structural Diversity. Frontiers in Microbiology 2019, 10, 791 .
AMA StyleSéverine Le Manach, Charlotte Duval, Arul Marie, Chakib Djediat, Arnaud Catherine, Marc Edery, Cecile Bernard, Benjamin Marie. Global Metabolomic Characterizations of Microcystis spp. Highlights Clonal Diversity in Natural Bloom-Forming Populations and Expands Metabolite Structural Diversity. Frontiers in Microbiology. 2019; 10 ():791.
Chicago/Turabian StyleSéverine Le Manach; Charlotte Duval; Arul Marie; Chakib Djediat; Arnaud Catherine; Marc Edery; Cecile Bernard; Benjamin Marie. 2019. "Global Metabolomic Characterizations of Microcystis spp. Highlights Clonal Diversity in Natural Bloom-Forming Populations and Expands Metabolite Structural Diversity." Frontiers in Microbiology 10, no. : 791.
With the increasing impact of the global warming, occurrences of cyanobacterial blooms in aquatic ecosystems are becoming a main worldwide ecological concern. Due to their capacity to produce potential toxic metabolites, interactions between the cyanobacteria, their cyanotoxins and the surrounding freshwater organisms have been investigated during the last past years. Non-targeted metabolomic analyses have the powerful capacity to study simultaneously a high number of metabolites and thus to investigate in depth the molecular signatures between various organisms encountering different environmental scenario, and potentially facing cyanobacterial blooms. In this way, the liver metabolomes of two fish species (Perca fluviatilis and Lepomis gibbosus) colonizing various peri-urban lakes of the Île-de-France region displaying high biomass of cyanobacteria, or not, were investigated. The fish metabolome hydrophilic fraction was analyzed by 1H NMR analysis coupled with Batman peak treatment for the quantification and the annotation attempt of the metabolites. The results suggest that similar metabolome profiles occur in both fish species, for individuals collected from cyanobacterial blooming lakes compared to organism from non-cyanobacterial dominant environments. Overall, such environmental metabolomic pilot study provides new research perspectives in ecology and ecotoxicology fields, and may notably provide new information concerning the cyanobacteria/fish ecotoxicological interactions.
Benoît Sotton; Alain Paris; Séverine Le Manach; Alain Blond; Charlotte Duval; Qin Qiao; Arnaud Catherine; Audrey Combes; Valérie Pichon; Cécile Bernard; Benjamin Marie. Specificity of the metabolic signatures of fish from cyanobacteria rich lakes. Chemosphere 2019, 226, 183 -191.
AMA StyleBenoît Sotton, Alain Paris, Séverine Le Manach, Alain Blond, Charlotte Duval, Qin Qiao, Arnaud Catherine, Audrey Combes, Valérie Pichon, Cécile Bernard, Benjamin Marie. Specificity of the metabolic signatures of fish from cyanobacteria rich lakes. Chemosphere. 2019; 226 ():183-191.
Chicago/Turabian StyleBenoît Sotton; Alain Paris; Séverine Le Manach; Alain Blond; Charlotte Duval; Qin Qiao; Arnaud Catherine; Audrey Combes; Valérie Pichon; Cécile Bernard; Benjamin Marie. 2019. "Specificity of the metabolic signatures of fish from cyanobacteria rich lakes." Chemosphere 226, no. : 183-191.
Lake Dziani Dzaha (Mayotte Island, Indian Ocean) is a tropical thalassohaline lake which geochemical and biological conditions make it a unique aquatic ecosystem considered as a modern analogue of Precambrian environments. In the present study, we focused on the diversity of phytoplanktonic communities, which produce very high and stable biomass (mean2014–2015 = 652 ± 179 μg chlorophyll a L−1). As predicted by classical community ecology paradigms, and as observed in similar environments, a single species is expected to dominate the phytoplanktonic communities. To test this hypothesis, we sampled water column in the deepest part of the lake (18 m) during rainy and dry seasons for two consecutive years. Phytoplanktonic communities were characterized using a combination of metagenomic, microscopy-based and flow cytometry approaches, and we used statistical modeling to identify the environmental factors determining the abundance of dominant organisms. As hypothesized, the overall diversity of the phytoplanktonic communities was very low (15 OTUs), but we observed a co-dominance of two, and not only one, OTUs, viz., Arthrospira fusiformis (Cyanobacteria) and Picocystis salinarum (Chlorophyta). We observed a decrease in the abundance of these co-dominant taxa along the depth profile and identified the adverse environmental factors driving this decline. The functional traits measured on isolated strains of these two taxa (i.e., size, pigment composition, and concentration) are then compared and discussed to explain their capacity to cope with the extreme environmental conditions encountered in the aphotic, anoxic, and sulfidic layers of the water column of Lake Dziani Dzaha.
C. Bernard; A. Escalas; N. Villeriot; H. Agogué; M. Hugoni; C. Duval; C. Carré; P. Got; G. Sarazin; D. Jézéquel; C. Leboulanger; V. Grossi; M. Ader; M. Troussellier. Very Low Phytoplankton Diversity in a Tropical Saline-Alkaline Lake, with Co-dominance of Arthrospira fusiformis (Cyanobacteria) and Picocystis salinarum (Chlorophyta). Microbial Ecology 2019, 78, 603 -617.
AMA StyleC. Bernard, A. Escalas, N. Villeriot, H. Agogué, M. Hugoni, C. Duval, C. Carré, P. Got, G. Sarazin, D. Jézéquel, C. Leboulanger, V. Grossi, M. Ader, M. Troussellier. Very Low Phytoplankton Diversity in a Tropical Saline-Alkaline Lake, with Co-dominance of Arthrospira fusiformis (Cyanobacteria) and Picocystis salinarum (Chlorophyta). Microbial Ecology. 2019; 78 (3):603-617.
Chicago/Turabian StyleC. Bernard; A. Escalas; N. Villeriot; H. Agogué; M. Hugoni; C. Duval; C. Carré; P. Got; G. Sarazin; D. Jézéquel; C. Leboulanger; V. Grossi; M. Ader; M. Troussellier. 2019. "Very Low Phytoplankton Diversity in a Tropical Saline-Alkaline Lake, with Co-dominance of Arthrospira fusiformis (Cyanobacteria) and Picocystis salinarum (Chlorophyta)." Microbial Ecology 78, no. 3: 603-617.
Among the diverse toxic components produced by cyanobacteria, microcystins (MCs) are one of the most toxic and notorious cyanotoxin groups. Besides their potent hepatotoxicity, MCs have been revealed to induce potential reproductive toxicity in various animal studies. However, little is still known regarding the distribution of MCs in the reproductive organ, which could directly affect reproductive cells. In order to respond to this question, an acute study was conducted in adult medaka fish (model animal) gavaged with 10 μg.g-1 body weight of pure MC-LR. The histological and immunohistochemical examinations reveal an intense distribution of MC-LR within hepatocytes along with a severe liver lesion in the toxin-treated female and male fish. Besides being accumulated in the hepatocytes, MC-LR was also found in the connective tissue of the ovary and the testis, as well as in oocytes and degenerative spermatocyte-like structures but not spermatocytes. Both liver and gonad play important roles in the reproductive process of oviparous vertebrates. This observation constitutes the first observation of the presence of MC-LR in reproductive cells (female, oocytes) of a vertebrate model with in vivo study. Our results, which provide intracellular localization of MC-LR in the gonad, advance our understanding of the potential reproductive toxicity of MC-LR in fish.
Qin Qiao; Chakib Djediat; Hélène Huet; Charlotte Duval; Séverine Le Manach; Cécile Bernard; Marc Edery; Benjamin Marie. Subcellular localization of microcystin in the liver and the gonads of medaka fish acutely exposed to microcystin-LR. Toxicon 2019, 159, 14 -21.
AMA StyleQin Qiao, Chakib Djediat, Hélène Huet, Charlotte Duval, Séverine Le Manach, Cécile Bernard, Marc Edery, Benjamin Marie. Subcellular localization of microcystin in the liver and the gonads of medaka fish acutely exposed to microcystin-LR. Toxicon. 2019; 159 ():14-21.
Chicago/Turabian StyleQin Qiao; Chakib Djediat; Hélène Huet; Charlotte Duval; Séverine Le Manach; Cécile Bernard; Marc Edery; Benjamin Marie. 2019. "Subcellular localization of microcystin in the liver and the gonads of medaka fish acutely exposed to microcystin-LR." Toxicon 159, no. : 14-21.
Thalassohaline ecosystems are hypersaline environments originating from seawater in which sodium chloride is the most abundant salt and the pH is alkaline. Studies focusing on microbial diversity in thalassohaline lakes are still scarce compared with those on athalassohaline lakes such as soda lakes that have no marine origin. In this work, we investigated multiple facets of bacterial, archaeal and eukaryotic diversity in the thalassohaline Lake Dziani Dzaha using a metabarcoding approach. We showed that bacterial and archaeal diversity were mainly affected by contrasting physicochemical conditions retrieved at different depths. While photosynthetic microorganisms were dominant in surface layers, chemotrophic phyla (Firmicutes or Bacteroidetes) and archaeal methanogens dominated deeper layers. In contrast, eukaryotic diversity was constant regardless of depth and was affected by seasonality. A detailed focus on eukaryotic communities showed that this constant diversity profile was the consequence of the high predominance of Picocystis salinarum, while nondominant eukaryotic groups displayed seasonal diversity turnover. Altogether, our results provided an extensive description of the diversity of the three domains of life in an unexplored extreme environment and showed clear differences in the responses of prokaryotic and eukaryotic communities to environmental conditions. This article is protected by copyright. All rights reserved.
Mylène Hugoni; Arthur Escalas; Cécile Bernard; Sébastien Nicolas; Didier Jézéquel; Fanny Vazzoler; Gerard Sarazin; Christophe Leboulanger; Marc Bouvy; Patrice Got; Magali Ader; Marc Troussellier; Hélène Agogué. Spatiotemporal variations in microbial diversity across the three domains of life in a tropical thalassohaline lake (Dziani Dzaha, Mayotte Island). Molecular Ecology 2018, 27, 4775 -4786.
AMA StyleMylène Hugoni, Arthur Escalas, Cécile Bernard, Sébastien Nicolas, Didier Jézéquel, Fanny Vazzoler, Gerard Sarazin, Christophe Leboulanger, Marc Bouvy, Patrice Got, Magali Ader, Marc Troussellier, Hélène Agogué. Spatiotemporal variations in microbial diversity across the three domains of life in a tropical thalassohaline lake (Dziani Dzaha, Mayotte Island). Molecular Ecology. 2018; 27 (23):4775-4786.
Chicago/Turabian StyleMylène Hugoni; Arthur Escalas; Cécile Bernard; Sébastien Nicolas; Didier Jézéquel; Fanny Vazzoler; Gerard Sarazin; Christophe Leboulanger; Marc Bouvy; Patrice Got; Magali Ader; Marc Troussellier; Hélène Agogué. 2018. "Spatiotemporal variations in microbial diversity across the three domains of life in a tropical thalassohaline lake (Dziani Dzaha, Mayotte Island)." Molecular Ecology 27, no. 23: 4775-4786.
With the increasing impact of the global warming, occurrences of cyanobacterial blooms in aquatic ecosystems are becoming a main ecological concern around the world. Due to their capacity to produce potential toxic metabolites, interactions between the cyanobacteria/cyanotoxin complex and the other freshwater organisms have been widely studied in the past years. Non-targeted metabolomic analyses have the powerful capacity to study a high number of metabolites at the same time and thus to understand in depth the molecular interactions between various organisms in different environmental scenario and notably during cyanobacterial blooms. In this way during summer 2015, liver metabolomes of two fish species, sampled in peri-urban lakes of the île-de-France region containing or not high concentrations of cyanobacteria, were studied. The results suggest that similar metabolome changes occur in both fish species exposed to cyanobacterial blooms compared to them not exposed. Metabolites implicated in protein synthesis, protection against ROS, steroid metabolism, cell signaling, energy storage and membrane integrity/stability have shown the most contrasted changes. Furthermore, it seems that metabolomic studies will provide new information and research perspectives in various ecological fields and notably concerning cyanobacteria/fish interactions but also a promising tool for environmental monitoring of water pollutions.
Benoit Sotton; Alain Paris; Severine Le Manach; Alain Blond; Charlotte Duval; Qin Qiao; Arnaud Catherine; Audrey Combes; Valerie Pichon; Cecile Bernard; Benjamin Marie. Specific metabolic signatures of fish exposed to cyanobacterial blooms. 2018, 416297 .
AMA StyleBenoit Sotton, Alain Paris, Severine Le Manach, Alain Blond, Charlotte Duval, Qin Qiao, Arnaud Catherine, Audrey Combes, Valerie Pichon, Cecile Bernard, Benjamin Marie. Specific metabolic signatures of fish exposed to cyanobacterial blooms. . 2018; ():416297.
Chicago/Turabian StyleBenoit Sotton; Alain Paris; Severine Le Manach; Alain Blond; Charlotte Duval; Qin Qiao; Arnaud Catherine; Audrey Combes; Valerie Pichon; Cecile Bernard; Benjamin Marie. 2018. "Specific metabolic signatures of fish exposed to cyanobacterial blooms." , no. : 416297.