The consumption of probiotics has been associated with a wide range of health benefits for consumers. Products containing probiotics need to have effective delivery of the microorganisms for their consumption to translate into benefits to the consumer. In the last few years, the microencapsulation of probiotic microorganisms has gained interest as a method to improve the delivery of probiotics in the host as well as extending the shelf life of probiotic-containing products. The microencapsulation of probiotics presents several aspects to be considered, such as the type of probiotic microorganisms, the methods of encapsulation, and the coating materials. The aim of this review is to present an updated overview of the most recent and common coating materials used for the microencapsulation of probiotics, as well as the involved techniques and the results of research studies, providing a useful knowledge basis to identify challenges, opportunities, and future trends around coating materials involved in the probiotic microencapsulation.

Trypanosoma cruziis the causal agent of Chagas’ disease which affects millions of people around the world mostly in Central and South America.T. cruziexpresses a wide variety of proteins on its surface membrane which has an important role in the biology of these parasites. Surface molecules of the parasites are the result of the environment to which the parasites are exposed during their life cycle. Hence,T. cruzidisplays several modifications when they move from one host to another. Due to the complexity of this parasite’s cell surface, this review presents some membrane proteins organized as large families, as they are the most abundant and/or relevant throughout theT. cruzimembrane.

Manganese peroxidases (MnP) from the white-rot fungi Phanerochaete chrysosporium catalyse the oxidation of Mn²⁺ to Mn³⁺, a strong oxidizer able to oxidize a wide variety of organic compounds. Different approaches have been used to unravel the enzymatic properties and potential applications of MnP. However, these efforts have been hampered by the limited production of native MnP by fungi. Heterologous expression of MnP has been achieved in both eukaryotic and prokaryotic expression systems, although with limited production and many disadvantages in the process. Here we described a novel molecular approach for the expression and purification of manganese peroxidase isoform 1 (MnP1) from P. chrysosporium using an E. coli-expression system. The proposed strategy involved the codon optimization and chemical synthesis of the MnP1 gene for optimised expression in the E. coli T7 shuffle host. Recombinant MnP1 (rMnP1) was expressed as a fusion protein, which was recovered from solubilised inclusion bodies. rMnP1 was purified from the fusion protein using intein-based protein purification techniques and a one-step affinity chromatography. The designated strategy allowed production of an active enzyme able to oxidize guaiacol or Mn²⁺.

Baking and brewing are among the oldest bioprocesses refined by human societies. Both fermentative processes have successfully used domesticated strains of Saccharomyces cerevisiae in their process as the biocatalyst throughout their evolution. However, the dominance of S. cerevisiae has limited the capability for diversification of many organoleptic properties of the final product, such as aroma and flavours. The use of non-Saccharomyces yeasts can be an enormous source of opportunities for innovation in both fermentative processes. Torulaspora delbrueckii is a ubiquitous yeast species, and numerous strains have been isolated from many different bioprocesses. The strains of T. delbrueckii, once considered microbial contamination, have recently shown several advantages over S. cerevisiae strains, including higher ethanol tolerance; better capabilities to consume wort sugars; higher resistance to hop/pH/osmotic stress; and freeze-thaw resistance, among others. This chapter aims to present a comprehensive review of frontier research on T. delbrueckii regarding its potential and prospects for the baking and brewing industries.