Jan Jakubík Obtained his PhD in 1997 from the First faculty of medicine, Charles University in Prague, Czech Republic. From 1997 to 1999 was visiting fellow at NIDDK, NIH, Bethesda, MD, USA and from 1999 to 2001 research assistant at the Medical School of the University of Minnesota, Minneapolis, MN, USA. Since 2016 he has been head of the Laboratory of Neurochemistry, Institute of Physiology, Czech Academy of Sciences, Prague, Czech Republic. His research is focused on i) Neurochemistry of the cholinergic system – Role of cholinergic neurotransmission in Alzheimer’s disease. Role of membrane cholesterol and lipids in cholinergic signalling. ii) Neuropharmacology of GPCRs – Atypical agonists. Functionally selective and biased agonists. Long-acting antagonists. PAM-antagonists. Bifunctional antagonists. iii) Receptor Theory – Allosteric modulation, The operational model of agonism, and Signalling bias. Among his achievements are: Conceptualization of signalling bias at the G-protein level, Discovery of long-acting muscarinic antagonists, Unravelling the role of cholesterol and lipids in cholinergic signalling, Establishing an integral part of the binding-pocket vestibule in receptor function, Discovery of positive allosteric modulators of acetylcholine, Discovery of allosteric agonists.