Whether animals have emotions was historically a long-lasting question but, today, nobody disputes that they do. However, how to assess them and how to guarantee animals their welfare have become important research topics in the last 20 years. Infrared thermography (IRT) is a method to record the electromagnetic radiation emitted by bodies. It can indirectly assess sympathetic and parasympathetic activity via the modification of temperature of different body areas, caused by different phenomena such as stress-induced hyperthermia or variation in blood flow. Compared to other emotional activation assessment methods, IRT has the advantage of being noninvasive, allowing use without the risk of influencing animals’ behavior or physiological responses. This review describes general principles of IRT functioning, as well as its applications in studies regarding emotional reactions of domestic animals, with a brief section dedicated to the experiments on wildlife; it analyzes potentialities and possible flaws, confronting the results obtained in different taxa, and discusses further opportunities for IRT in studies about animal emotions.

Blinking, along with other facial expressions, has been suggested to play a role in dogs’ intra- and interspecific communication, however the feedback this signal elicits from the audience is still poorly studied. In this study, we investigated the behavioural and physiological responses of 54 domestic dogs to videos of conspecifics performing blink. Based on existing literature, we hypothesized that dogs would show a higher rate of blinking when exposed to blink than to another facial expression (nose lick) and to an attentive still-looking face (control). Results showed that dogs blinked more during the blink video compared to the nose lick (NL) video, suggesting a mimicry phenomenon and implying a possible role of blinking in dogs’ communication. Cardiac analyses showed increased heart rate variability values during the video sessions independently to the type of facial signal projected, suggesting that the stimuli were not perceived as stressful. The present results open the door to future investigation of blink synchronization, as this aspect was not directly addressed in the present study. Future research should also explore the effects of eye blink and NL in modulating intraspecific social interactions.

Shelters are stressful environments for domestic dogs which are known to negatively impact their welfare. The introduction of outside stimuli for dogs in this environment can improve their welfare and life conditions. However, our current understanding of the influence of different stimuli on shelter dogs’ welfare is limited and the data is still insufficient to draw conclusions. In this study, we collected 28 days (four weeks) of telemetry data from eight male dogs housed in an Italian shelter for a long period of time. During this period, three types of enrichment were introduced into the dogs’ pens for one week each: entertaining objects, intraspecific, and interspecific social enrichment, by means of the presence of female conspecifics and the presence of a human. To quantify their impact, we introduce novel metrics as indicators of sheltered dogs’ welfare based on telemetry data: the variation of heart rate, muscle activity, and body temperature from an average baseline day, quality of sleep, and the regularity for cyclicity of the aforementioned parameters, based on the day-night cycle. Using these metrics, we show that while all three stimuli statistically improve the dogs’ welfare, the variance between individual dogs is large. Moreover, our findings indicate that the presence of female conspecific is the best stimulus among the three explored options which improves both the quality of sleep and the parameters’ cyclicity. Our results are consistent with previous research findings while providing novel data-driven welfare indicators that promote objectivity. Thus, this research provides some useful guidelines for managing shelters and improving dogs’ welfare.

The Italian wolf Canis lupus italicus faced near extinction in the 19th and early 20th centuries due to extensive persecution, habitat degradation, and prey depletion. Over the past 50 years, conservation efforts, legal protection, and habitat recovery have supported a resurgence, with recent estimates placing the population at approximately 3,300 individuals. Wolves have expanded into lowlands, plains, and coastal areas, including the highly anthropised Po Plain. This expansion intensifies human-wildlife conflicts, particularly in livestock farming and hunting. The invasive coypu (Myocastor coypus) and eastern cottontail (Sylvilagus floridanus) have inflicted considerable ecological and economic damage in northern Italy. These populations are poorly controlled due to their ecological plasticity and lack of natural predators. Given the adaptability of wolves and their opportunistic diet, this study investigates whether they could become a significant prey species for wolves in plains environments, potentially contributing to their control while mitigating livestock predation. Through genetic analysis, scat morphology, and camera trapping, this study confirms the presence of coypu in the diet of a wolf pack in the Torrile and Trecasali Natural Reserve. These findings highlight the potential ecological role of wolves as indirect allies in managing invasive species, which could help alleviate economic and physical damage to agriculture and riverbanks, fostering more positive public attitudes and greater acceptance of the species.