River and open-channel flows present complex three-dimensional (3D) structures in the water column and on the surface as a result of the existence of secondary currents driven by either centrifugal force or turbulence. This paper experimentally investigates secondary currents in a straight, turbulent, rough-bed open-channel flow; the effect of different channel width to flow depth ratios (12.25, 15, and 20) on secondary currents; and surface boils associated with secondary currents at moderate Reynolds numbers. Nearly instantaneous profiles of three components of flow velocity and turbulence characteristics in the water column were measured by using an acoustic Doppler velocity profiler (ADVP). Simultaneously, large-scale particle image velocimetry was used to measure the water surface velocities and turbulence structures. Mean velocity patterns in the water undulate across the channel, indicating the presence of secondary currents in the long-term average flow structure. Secondary currents affect the dis...