Microbiomes are important for crop performance. However, a deeper knowledge of crop-associated microbial communities is needed to harness beneficial host-microbe interactions. Here, by assessing the assembly and functions of maize microbiomes across soil types, climate zones, and genotypes, we found that the stem xylem selectively recruits highly conserved microbes dominated by Gammaproteobacteria. We showed that the proportion of bacterial taxa carrying the nitrogenase gene (nifH) was larger in stem xylem than in other organs such as root and leaf endosphere. Of the 25 core bacterial taxa identified in xylem sap, several isolated strains were confirmed to be active nitrogen-fixers or to assist with biological nitrogen fixation. On this basis, we established synthetic communities (SynComs) consisting of two core diazotrophs and two helpers. GFP-tagged strains and ¹⁵N isotopic dilution method demonstrated that these SynComs do thrive and contribute, through biological nitrogen fixation, 11.8% of the total N accumulated in maize stems. These core taxa in xylem sap represent an untapped resource that can be exploited to increase crop productivity.

Belowground nitrogen (N) transfer from legumes to non-legumes provides an important N source for crop yield and N utilization. However, whether root contact facilitates N transfer and the extent to which N transfer contributes to crop productivity and N utilization have not been clarified. In our study, two-year rain shelter experiments were conducted to quantify the effect of root contact on N transfer in a maize/alfalfa intercropping system. N transfer occurred mainly one direction from alfalfa to maize during the growth period. Following the N0 treatment, the amount of N transfer from alfalfa to maize was 204.56 mg pot⁻¹ with no root barrier and 165.13 mg pot⁻¹ with a nylon net barrier, accounting for 4.72% and 4.48% of the total N accumulated in maize, respectively. Following the N1 treatment, the amount of N transfer from alfalfa to maize was 197.70 mg pot⁻¹ with no root barrier and 139.04 mg pot⁻¹ with a nylon net barrier, accounting for 3.64% and 2.36% of the total N accumulated in the maize, respectively. Furthermore, the amount of N transfer without no root barrier was 1.24–1.42 times higher than that with a nylon net barrier regardless of the level of N addition. Our results highlight the importance and the relevance of root contact for the enhancement of N transfer in a maize/alfalfa intercropping system.

Ridge tillage and straw returning are tillage practices widely used in the Chinese Mollisol region. However, the effects of ridge tillage combined with straw returning on runoff and soil loss control are still unclear. The objective of this study was to compare the effects of ridge tillage practices (contour ridge (CR)) and longitudinal ridge (LR), straw returning practices (straw on the furrow surface (SS)) and straw below the furrow (SB)), and their interactions on the runoff and soil loss by using simulated rainfall experiment. Two rainfall intensities (45 and 60 mm h⁻¹) were applied to six combinations of ridge tillage and straw returning (contour ridge treatment, contour ridge with straw on the furrow surface treatment, contour ridge with straw below the furrow treatment, longitudinal ridge treatment, longitudinal ridge with straw on the furrow surface treatment, and longitudinal ridge with straw below the furrow treatment) on a 5° slope. The results showed that the phenomenon of ridge failure was common in the treatments with contour ridge. The average runoff rate and soil loss rate after ridge failure for treatments with contour ridge were separated 2.8 and 3.5 times greater than those of before failure at 60 mm h⁻¹. However, the corresponding values were only 68.6% and 43.3% of the average value of longitudinal ridge treatment and longitudinal ridge with straw below the furrow treatment at 60 mm h⁻¹. The water storage capacities of treatments with contour ridge remained constant when the rainfall intensity varied. The water storage capacities of contour ridge with straw on and below the furrow treatments were separate 3.0 and 1.0 mm less than that of contour ridge. However, longitudinal ridge with straw on the furrow surface treatment increased the runoff rate by 7.4% but reduced the soil loss rate by 72.6% when compared with longitudinal ridge treatment and longitudinal ridge with straw below the furrow treatment under the two rainfall intensities. Longitudinal with straw on the furrow surface treatment was more conducive to the stability of ridges, and there was no significant difference in total soil loss between longitudinal ridge with straw on the furrow surface treatment and treatments with contour ridge. This study was based on simulated rainfall conditions, and its adaptability under long-term positioning monitor in the field should be added in future.