This study aimed to systematically assess the phenolic profiles and antioxidant capacities of 21 chestnut samples collected from six geographical areas of China. All these samples exhibit significant differences (p < 0.05) in total phenolic contents (TPC), total flavonoids content (TFC), condensed tannin content (CTC) and antioxidant capacities assessed by DPPH free radical scavenging capacity (DPPH), ABTS free radical scavenging capacities (ABTS), ferric reducing antioxidant power (FRAP), and 14 free phenolic acids. Chestnuts collected from Fuzhou, Jiangxi (East China) exhibited the maximum values for TPC (2.35 mg GAE/g), CTC (13.52 mg CAE/g), DPPH (16.74 μmol TE/g), ABTS (24.83 μmol TE/g), FRAP assays (3.20 mmol FE/100 g), and total free phenolic acids (314.87 µg/g). Vanillin and gallic acids were found to be the most abundant free phenolic compounds among other 14 phenolic compounds detected by HPLC. Overall, the samples from South China revealed maximum mean values for TPC, CTC, DPPH, and ABTS assays. Among the three chestnut varieties, Banli presented prominent mean values for all the assays. These finding will be beneficial for production of novel functional food and developing high-quality chestnut varieties.
To test the hypothesis that migration from the thermal labels on plastic film packaging is a major source of exposure to bisphenols and alternative color developers in food, we analyzed 140 packaging materials from packaged fresh food purchased in North America. No bisphenol A (BPA) was detected in either the packaging samples or thermal labels. However, significant amounts of bisphenol S (BPS) and alternative color developers (up to 214 μg/cm2) were present in thermal labels; their relative occurrence varied among stores. In a controlled experiment, we wrapped fish in film with a thermal label for five days at 4℃. The fish in contact with the label contained BPS (≤ 1140 ng/g wet weight [ww]), 4-hydroxyphenyl 4-isoprooxyphenylsulfone (D-8) (≤ 230 ng/g ww), bis(2-chloroethyl)ether-4,400-dihydroxydiphenyl sulfone monomer (D-90) (≤ 3.41 ng/g ww), and Pergafast-201 (≤ 1.87 ng/g ww). This study provides evidence, for the first time, that BPS and alternative thermal label color developers migrate from packaging materials into food. Further, BPS migration significantly exceeded the European Union Specific Migration Limit (50 ng/g ww), suggesting that further risk assessment studies are warranted.