Research Highlights: Mangrove forests are absorbing anthropogenically produced excess nitrogen under moderate to intensive human interaction in the study sites, further indicating the degree of deviation from the natural ecosystem condition. Background and Objectives: Mangrove species, when directly connected to anthropogenic activities such as sewage disposal, agricultural inputs, and receiving of animal manure, absorb excess nutrients from the systems and act as ecological indicators of long-term natural changes. However, there is a paucity of examples of how the mangroves respond to a land-use gradient comparing to the non-mangrove plants under indirect anthropogenic impacts. Materials and Methods: In this investigation, foliar total nitrogen (N), carbon to nitrogen (C/N) ratio, and δ¹⁵N of mangrove and non-mangrove species collected from 15 watersheds on three islands in Okinawa, Japan, have been compared. The land-use areas in the study watersheds were delineated by ArcGIS software, and the correlation between the foliar traits and the human-affected area ratios were examined. Results: Foliar δ¹⁵N of the mangroves, which was significantly different from those of the non-mangroves on each island, showed significantly higher values (5‰ to 14‰) in human-affected forests, whereas the values were up to 3‰ in pristine forests. Furthermore, the significant positive relationship between foliar nitrogenous traits and the human-affected area ratios suggested that the anthropogenic N might be regulating foliar N content and δ¹⁵N signature on the sites. Conclusion: Different degrees of foliar isotopic fractionation with the land-use gradient have clarified that mangroves can be a powerful tool for monitoring ecosystem conditions under anthropogenic disturbances.

Iron solubilization in mangrove soils associated with polyphenols leached out from leaf-litter can improve iron bioavailability. In this context, the leaf-removing process by mangrove crabs would increase reacting frequency of the polyphenols in mangrove leaves with iron in the soils. In this study, we investigated ecological roles of a leaf-removing crab, Neosarmatium smithi, on the iron solubilization process. After the fallen leaves carried by the crabs to their burrows and eaten by them, polyphenols may be remained in their feces. If so, contact of polyphenols in the feces with mangrove soils could promote elution of dissolved iron from the soils. In order to demonstrate this hypothesis, we firstly surveyed the appearance ratio of the black part in crab burrows and measured total phenolic content in feces of N. smithi as well as in the black part soil. Then, we examined influences of the crab feces on dissolved iron elution from mangrove soils. As the results, the appearance ratio of the black part in the burrow was 67% and the phenolic content in the feces, the black part, and the yellow part in crab burrows were 9.93, 0.49, and 0.12mg g－1, respectively. Dissolved iron content in the solution (soil＋water extract from feces) was 0.65μg g－1 and this content was 4.5 times higher than the control (soil＋distilled water). We suggest that the polyphenols remained in the feces affect to solubilize insoluble forms of iron by iron reduction and chelating properties.

This study evaluated the freshwater use of two mangrove species (R. mucronate: Rhizophora mucronata and A. marina: Avicennia marina) growing in a coastal area of Djibouti City and on Moucha Island in Djibouti. We accomplished this by comparing hydrogen and oxygen stable isotope ratios (δ²H and δ¹⁸O) in the body water with those of creek water, soil water, groundwater, and rainwater samples. Line-conditioned excess (lc-excess) was calculated for the δ²H and δ¹⁸O values of the groundwater and rainwater samples. Although two regression lines were drawn using the δ²H and δ¹⁸O values of the plant body water as well as the environmental water, no significant differences were found, suggesting that the effects of isotope fractionation due to evaporation in all the samples were almost similar. Comparison of lc-excess and δ¹⁸O suggested that the body water from the two mangrove species growing on Moucha Is. were strongly influenced by seawater, but contained some freshwater sources other than the seawater, the soil water, and the creek water. It was also found that Avicennia marina growing in the coastal area was strongly influenced by groundwater. The origin of the freshwater absorbed by the mangroves on the island was not clear, but it could be groundwater recharged in a faraway place.