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The blackwater stream of domestic wastewater contains energy and the majority of nutrients that can contribute to a circular economy. Hygienically safe and odor-free nutrient solution produced from anaerobically treated source-separated blackwater through an integrated post-treatment unit can be used as a source of liquid fertilizer. However, the high water content in the liquid fertilizer represents a storage or transportation challenge when utilized on agricultural areas, which are often situated far from the urban areas. Integration of microalgae into treated source-separated blackwater (BW) has been shown to effectively assimilate and recover phosphorus (P) and nitrogen (N) in the form of green biomass to be used as slow release biofertilizer and hence close the nutrient loop. With this objective, a lab-scale flat panel photobioreactor was used to cultivate Chlorella sorokiniana strain NIVA CHL 176 in a chemostat mode of operation. The growth of C. sorokiniana on treated source-separated blackwater as a substrate was monitored by measuring dry biomass concentration at a dilution rate of 1.38 d−1, temperature of 37 °C and pH of 7. The results indicate that the N and P recovery rates of C. sorokiniana were 99 mg N L−1d−1 and 8 mg P L−1d−1 for 10% treated BW and reached 213 mg N L−1d−1 and 35 mg P L−1d−1, respectively when using 20% treated BW as a substrate. The corresponding biomass yield on light, N and P on the 20% treated BW substrate were 0.37 g (mol photon)−1, 9.1 g g−1 and 54.1 g g−1, respectively, and up to 99% of N and P were removed from the blackwater.
Melesse Eshetu Moges; Arve Heistad; Thorsten Heidorn. Nutrient Recovery from Anaerobically Treated Blackwater and Improving Its Effluent Quality through Microalgae Biomass Production. Water 2020, 12, 592 .
AMA StyleMelesse Eshetu Moges, Arve Heistad, Thorsten Heidorn. Nutrient Recovery from Anaerobically Treated Blackwater and Improving Its Effluent Quality through Microalgae Biomass Production. Water. 2020; 12 (2):592.
Chicago/Turabian StyleMelesse Eshetu Moges; Arve Heistad; Thorsten Heidorn. 2020. "Nutrient Recovery from Anaerobically Treated Blackwater and Improving Its Effluent Quality through Microalgae Biomass Production." Water 12, no. 2: 592.
Nitrogenase based hydrogen production was examined in a ΔhupW strain of the filamentous heterocystous cyanobacterium Nostoc PCC 7120, i.e., cells lacking the last step in the maturation system of the large subunit of the uptake hydrogenase and as a consequence with a non-functional uptake hydrogenase. The cells were grown in a developed flat panel photobioreactor system with 3.0L culture volume either aerobically (air) or anaerobically (Ar or 80% N2/20% Ar) and illuminated with a mixture of red and white LED. Aerobic growth of the ΔhupW strain of Nostoc PCC 7120 at 44μmolar photons m(-2)s(-1) PAR gave the highest hydrogen production of 0.7mL H2 L(-1)h(-1), 0.53mmol H2 mg chlorophyll a(-1)h(-1), and a light energy conversion efficiency of 1.2%. Anaerobic growth using 100% argon showed a maximal hydrogen production of 1.7mLL(-1)h(-1), 0.85mmol per mg chlorophyll a(-1) h(-1), and a light energy conversion efficiency of 2.7%. Altering between argon/N2 (20/80) and 100% argon phases resulted in a maximal hydrogen production at hour 128 (100% argon phase) with 6.2mL H2L(-1)h(-1), 0.71mL H2 mg chlorophyll a(-1)h(-1), and a light energy efficiency conversion of 4.0%. The highest buildup of hydrogen gas observed was 6.89% H2 (100% argon phase) of the total photobioreactor system with a maximal production of 4.85mL H2 L(-1)h(-1). The present study clearly demonstrates the potential to use purpose design cyanobacteria in developed flat panel photobioreactor systems for the direct production of the solar fuel hydrogen. Further improvements in the strain used, environmental conditions employed, and growth, production and collection systems used, are needed before a sustainable and economical cyanobacterial based hydrogen production can be realized.
Marcus Nyberg; Thorsten Heidorn; Peter Lindblad. Hydrogen production by the engineered cyanobacterial strain Nostoc PCC 7120 ΔhupW examined in a flat panel photobioreactor system. Journal of Biotechnology 2015, 215, 35 -43.
AMA StyleMarcus Nyberg, Thorsten Heidorn, Peter Lindblad. Hydrogen production by the engineered cyanobacterial strain Nostoc PCC 7120 ΔhupW examined in a flat panel photobioreactor system. Journal of Biotechnology. 2015; 215 ():35-43.
Chicago/Turabian StyleMarcus Nyberg; Thorsten Heidorn; Peter Lindblad. 2015. "Hydrogen production by the engineered cyanobacterial strain Nostoc PCC 7120 ΔhupW examined in a flat panel photobioreactor system." Journal of Biotechnology 215, no. : 35-43.