To improve land use efficiency, urban renewal must also consider urban microclimates and heat islands. Existing research has depended on manual interpretation of high-resolution optical satellite imagery to resolve land surface temperature (LST) changes caused by urban renewal; however, the acquired ground time series data tend to be uneven and unique to specific frameworks. The objective of this study was to establish a more general framework to study LST changes caused by urban renewal using multi-source remote sensing data. Specifically, urban renewal areas during 2007–2017 were obtained by integrating Landsat and yearly Phased Array type L-band Synthetic Aperture Radar (PALSAR) images, and LST was retrieved from Landsat thermal infrared data using the generalized single-channel algorithm. Our results showed that urban renewal land (URL) area accounted for 1.88% of urban land area. Relative LST between URL and general urban land (GUL) of Liwan, Yuexiu, Haizhu, and Tianhe districts dropped by 0.88, 0.42, 0.43, and 0.10 K, respectively, whereas those of Baiyun, Huangpu, Panyu, and Luogang districts presented opposite characteristics, with a rise in the LST of 0.98, 1.03, 1.63, and 2.11 K, respectively. These results are attributable to population density, building density, and landscape pattern changes during the urban renewal process.