Nathalie Rouach

Dr. Nathalie Rouach

Center for Interdisciplinary Research in Biology, Paris, France
orcid

Research Focus & Metrics

Academic Career Length

27Years (2000-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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15 February 2021 in Nature Neuroscience
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Journal Article
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CECarole Escartin
Carole Escartin
EGElena Galea
Elena Galea
ALAndrás Lakatos
András Lakatos
JOJames P. O’cAllaghan
James P. O’cAllaghan
GPGabor C. Petzold
Gabor C. Petzold
Alexei VerkhratskyAlexei Verkhratsky
Alexei Verkhratsky

5 December 2008 in Science
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Journal Article
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NRNathalie Rouach
Nathalie Rouach
AKAnnette Koulakoff
Annette Koulakoff
VAVeronica Abudara
Veronica Abudara
KWKlaus Willecke
Klaus Willecke
CGChristian Giaume
Christian Giaume
Astrocytes provide metabolic substrates to neurons in an activity-dependent manner. However, the molecular mechanisms involved in this function, as well as its role in synaptic transmission, remain unclear. Here, we show that the gap-junction subunit proteins connexin 43 and 30 allow intercellular trafficking of glucose and its metabolites through astroglial networks. This trafficking is regulated by glutamatergic synaptic activity mediated by AMPA receptors. In the absence of extracellular glucose, the delivery of glucose or lactate to astrocytes sustains glutamatergic synaptic transmission and epileptiform activity only when they are connected by gap junctions. These results indicate that astroglial gap junctions provide an activity-dependent intercellular pathway for the delivery of energetic metabolites from blood vessels to distal neurons.

1 February 2010 in Nature Reviews Neuroscience
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CGChristian Giaume
Christian Giaume
AKAnnette Koulakoff
Annette Koulakoff
LRLisa Roux
Lisa Roux
DHDavid Holcman
David Holcman
NRNathalie Rouach
Nathalie Rouach

9 March 2017 in Brain Structure and Function
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FVFlora Vasile
Flora Vasile
EDElena Dossi
Elena Dossi
NRNathalie Rouach
Nathalie Rouach
Data collected on astrocytes’ physiology in the rodent have placed them as key regulators of synaptic, neuronal, network, and cognitive functions. While these findings proved highly valuable for our awareness and appreciation of non-neuronal cell significance in brain physiology, early structural and phylogenic investigations of human astrocytes hinted at potentially different astrocytic properties. This idea sparked interest to replicate rodent-based studies on human samples, which have revealed an analogous but enhanced involvement of astrocytes in neuronal function of the human brain. Such evidence pointed to a central role of human astrocytes in sustaining more complex information processing. Here, we review the current state of our knowledge of human astrocytes regarding their structure, gene profile, and functions, highlighting the differences with rodent astrocytes. This recent insight is essential for assessment of the relevance of findings using animal models and for comprehending the functional significance of species-specific properties of astrocytes. Moreover, since dysfunctional astrocytes have been described in many brain disorders, a more thorough understanding of human-specific astrocytic properties is crucial for better-adapted translational applications.

2 May 2011 in Proceedings of the National Academy of Sciences
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UPUlrike Pannasch
Ulrike Pannasch
LVLydia Vargová
Lydia Vargová
JRJürgen Reingruber
Jürgen Reingruber
PEPascal Ezan
Pascal Ezan
DHDavid Holcman
David Holcman
NRNathalie Rouach
Nathalie Rouach
Astrocytes dynamically interact with neurons to regulate synaptic transmission. Although the gap junction proteins connexin 30 (Cx30) and connexin 43 (Cx43) mediate the extensive network organization of astrocytes, their role in synaptic physiology is unknown. Here we show, by inactivating Cx30 and Cx43 genes, that astroglial networks tone down hippocampal synaptic transmission in CA1 pyramidal neurons. Gap junctional networking facilitates extracellular glutamate and potassium removal during synaptic activity through modulation of astroglial clearance rate and extracellular space volume. This regulation limits neuronal excitability, release probability, and insertion of postsynaptic AMPA receptors, silencing synapses. By controlling synaptic strength, connexins play an important role in synaptic plasticity. Altogether, these results establish connexins as critical proteins for extracellular homeostasis, important for the formation of functional synapses.

2 March 2014 in Nature Neuroscience
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Journal Article
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UPUlrike Pannasch
Ulrike Pannasch
DFDominik Freche
Dominik Freche
GDGlenn Dallérac
Glenn Dallérac
GGGrégory Ghézali
Grégory Ghézali
CECarole Escartin
Carole Escartin
NRNathalie Rouach
Nathalie Rouach