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Much attention is paid to the relationship between bacteria and plants in the process of the bioremediation of oil-contaminated soils, but the effect of petroleum degrading bacteria that synthesize phytohormones on the content and distribution of these compounds in plants is poorly studied. The goal of the present field experiment was to study the effects of hydrocarbon-oxidizing bacteria that produce auxins on the growth, biochemical characteristics, and hormonal status of barley plants in the presence of oil, as well as assessing the effect of bacteria and plants separately and in association with the content of oil hydrocarbons in the soil. The treatment of plants with strains of Enterobacter sp. UOM 3 and Pseudomonas hunanensis IB C7 led to an increase in the length and mass of roots and shoots and the leaf surface index, and an improvement in some parameters of the elements of the crop structure, which were suppressed by the pollutant. The most noticeable effect of bacteria on the plant hormonal system was a decrease in the accumulation of abscisic acid. The data obtained indicate that the introduction of microorganisms weakened the negative effects on plants under abiotic stress caused by the presence of oil. Plant-bacteria associations were more effective in reducing the content of hydrocarbons in the soil and increasing its microbiological activity than when either organism was used individually.
Elena Kuzina; Gulnaz Rafikova; Lidiya Vysotskaya; Tatyana Arkhipova; Margarita Bakaeva; Dar’Ya Chetverikova; Guzel Kudoyarova; Tatyana Korshunova; Sergey Chetverikov. Influence of Hydrocarbon-Oxidizing Bacteria on the Growth, Biochemical Characteristics, and Hormonal Status of Barley Plants and the Content of Petroleum Hydrocarbons in the Soil. Plants 2021, 10, 1745 .
AMA StyleElena Kuzina, Gulnaz Rafikova, Lidiya Vysotskaya, Tatyana Arkhipova, Margarita Bakaeva, Dar’Ya Chetverikova, Guzel Kudoyarova, Tatyana Korshunova, Sergey Chetverikov. Influence of Hydrocarbon-Oxidizing Bacteria on the Growth, Biochemical Characteristics, and Hormonal Status of Barley Plants and the Content of Petroleum Hydrocarbons in the Soil. Plants. 2021; 10 (8):1745.
Chicago/Turabian StyleElena Kuzina; Gulnaz Rafikova; Lidiya Vysotskaya; Tatyana Arkhipova; Margarita Bakaeva; Dar’Ya Chetverikova; Guzel Kudoyarova; Tatyana Korshunova; Sergey Chetverikov. 2021. "Influence of Hydrocarbon-Oxidizing Bacteria on the Growth, Biochemical Characteristics, and Hormonal Status of Barley Plants and the Content of Petroleum Hydrocarbons in the Soil." Plants 10, no. 8: 1745.
Plant-bacteria consortia are more effective in bioremediation of petroleum contaminated soil than when either organism is used individually. The reason for this is that plant root exudates promote growth and activity of oil degrading bacteria. However, insufficient attention has been paid to the ability of bacteria to influence root exudation. Therefore, the influence of barley plants and/or bacterial inoculation (Pseudomonas hunanensis IB C7 and Enterobacter sp. UOM 3) on the content of organic acids, sugars and plant hormones in the eluate from clean and oil-polluted sand was studied separately or in combination. These strains are capable of oxidizing hydrocarbons and synthesizing auxins. Concentrations of organic acids and sugars were determined using capillary electrophoresis, and hormones by enzyme-linked immunosorbent assays. In the absence of plants, no sugars were detected in the sand, confirming that root exudates are their main source. Introducing bacteria into the sand increased total contents of organic compounds both in the presence and absence of oil. This increase could be related to the increase in auxin amounts in the sand eluate, as well as in plants. The results indicate that bacteria are able to increase the level of root exudation. Since auxins can promote root exudation, bacterial production of this hormone is likely responsible for increased concentrations of soluble organic compounds in the sand. Bacterial mediation of root exudation by affecting plant hormonal status should be considered when choosing microorganisms for phytoremediation.
Sergey Chetverikov; Lidiya Vysotskaya; Elena Kuzina; Tatiana Arkhipova; Margarita Bakaeva; Gulnaz Rafikova; Tatiana Korshunova; Darya Chetverikova; Gaisar Hkudaygulov; Guzel Kudoyarova. Effects of Association of Barley Plants with Hydrocarbon-Degrading Bacteria on the Content of Soluble Organic Compounds in Clean and Oil-Contaminated Sand. Plants 2021, 10, 975 .
AMA StyleSergey Chetverikov, Lidiya Vysotskaya, Elena Kuzina, Tatiana Arkhipova, Margarita Bakaeva, Gulnaz Rafikova, Tatiana Korshunova, Darya Chetverikova, Gaisar Hkudaygulov, Guzel Kudoyarova. Effects of Association of Barley Plants with Hydrocarbon-Degrading Bacteria on the Content of Soluble Organic Compounds in Clean and Oil-Contaminated Sand. Plants. 2021; 10 (5):975.
Chicago/Turabian StyleSergey Chetverikov; Lidiya Vysotskaya; Elena Kuzina; Tatiana Arkhipova; Margarita Bakaeva; Gulnaz Rafikova; Tatiana Korshunova; Darya Chetverikova; Gaisar Hkudaygulov; Guzel Kudoyarova. 2021. "Effects of Association of Barley Plants with Hydrocarbon-Degrading Bacteria on the Content of Soluble Organic Compounds in Clean and Oil-Contaminated Sand." Plants 10, no. 5: 975.
The phytoremediation of soil contaminated with petroleum oil products relies on co-operation between plants and rhizosphere bacteria, including the plant growth-promoting effect of the bacteria. We studied the capacity of strains of Pseudomonas, selected as oil degraders, to produce plant hormones and promote plant growth. Strains with intermediate auxin production were the most effective in stimulating the seedling growth of seven plant species under normal conditions. Bacterial seed treatment resulted in about a 1.6-fold increase in the weight of barley seedlings, with the increment being much lower in other plant species. The strains P. plecoglossicida 2.4-D and P. hunanensis IB C7, characterized by highly efficient oil degradation (about 70%) and stable intermediate in vitro auxin production in the presence of oil, were selected for further study with barley. These strains increased the seed germination percentage approximately two-fold under 5% oil concentration in the soil, while a positive effect on further seedling growth was significant when the oil concentration was raised to 8%. This resulted in a 1.3–1.7-fold increase in the seedling mass after 7 days of growth, depending on the bacterial strain. Thus, strains of oil-degrading bacteria selected for their intermediate and stable production of auxin were found to be effective ameliorators of plant growth inhibition resulting from petroleum stress.
Margarita Bakaeva; Elena Kuzina; Lidiya Vysotskaya; Guzel Kudoyarova; Tat’Yana Arkhipova; Gulnaz Rafikova; Sergey Chetverikov; Tat’Yana Korshunova; Dar'ya Chetverikova; Oleg Loginov. Capacity of Pseudomonas Strains to Degrade Hydrocarbons, Produce Auxins and Maintain Plant Growth under Normal Conditions and in the Presence of Petroleum Contaminants. Plants 2020, 9, 379 .
AMA StyleMargarita Bakaeva, Elena Kuzina, Lidiya Vysotskaya, Guzel Kudoyarova, Tat’Yana Arkhipova, Gulnaz Rafikova, Sergey Chetverikov, Tat’Yana Korshunova, Dar'ya Chetverikova, Oleg Loginov. Capacity of Pseudomonas Strains to Degrade Hydrocarbons, Produce Auxins and Maintain Plant Growth under Normal Conditions and in the Presence of Petroleum Contaminants. Plants. 2020; 9 (3):379.
Chicago/Turabian StyleMargarita Bakaeva; Elena Kuzina; Lidiya Vysotskaya; Guzel Kudoyarova; Tat’Yana Arkhipova; Gulnaz Rafikova; Sergey Chetverikov; Tat’Yana Korshunova; Dar'ya Chetverikova; Oleg Loginov. 2020. "Capacity of Pseudomonas Strains to Degrade Hydrocarbons, Produce Auxins and Maintain Plant Growth under Normal Conditions and in the Presence of Petroleum Contaminants." Plants 9, no. 3: 379.