Urban vegetation is important in providing ecosystem services to people. Different urban vegetation types provide contrasting suites of ecosystem services and disservices. Understanding public perceptions of the ecosystem services and disservices can therefore play an important role in shaping the planning and management of urban areas. We conducted an online survey (n = 1000) to understand how residents in the tropical city of Singapore perceived urban vegetation and the associated ecosystem services and disservices. The questionnaire was designed to explore whether different urban vegetation types (grass, shrubs, trees, trees over shrubs, and secondary forest) were perceived as equal in providing benefits. Respondents considered ecosystem services provided by urban vegetation to be more important than disservices. Among ecosystem services, regulating services were most highly rated, with more than 80% of the respondents appreciating urban vegetation for providing shade and improving air quality. Respondents recognized that different vegetation types provided different ecosystem services. For example, secondary forest was most commonly associated with education and wildlife, while trees were strongly associated with cooling and air quality. We conclude that in developing plans and designs for urban vegetation and ecosystem services, it is important to understand the perceptions, priorities, and concerns of residents.