Habitat quality (HQ) has been progressively degrading worldwide in recent decades due to rapid climate change and intensive human activities. These changes not only threaten biodiversity and ecosystem functions, but also impact socio-economic development. Therefore, a few studies have focused on the dynamics of HQ and its natural and anthropogenic drivers. However, many contributions have failed to reveal how these factors interact to impact HQ, especially in ecologically fragile areas. We estimated HQ in the Songnen Plain of Northeast China, an ecologically fragile area, from 2000 to 2020 using the InVEST model and explored the response of HQ to the interactions of natural factors (topography, climate, NDVI) and anthropogenic factors (nighttime light index, population density) influencing HQ using Structural Equation Modelling (SEM). The results showed that 1) HQ decreased constantly from 2000 to 2018, and then increased slightly from 2018 to 2020. 2) In terms of spatial distribution, HQ appeared to be highly heterogeneous with a pattern of ‘high HQ in the east – low HQ in the center – high HQ in the west’ at each time point. The high-HQ areas were significantly clustered in the eastern parts with dense forests, while the low-HQ areas in the central parts were dominated by a large number of man-made patches of agriculture and towns or cities. 3) The spatial patterns of HQ are mainly affected by the interactions of factors including the natural environment and human disturbance. Natural factors had a greater impact on HQ than human disturbance, and human disturbance factors had significant negative impact among all these factors at 4 time points. Furthermore, the intensity of the impact of various influencing factors on habitat quality, as well as the positive or negative effects of these drivers on habitat quality, changed over time. The most important influencing factor was temperature in 2000 and topography in 2010, 2018, and 2020. This study can provide important suggestions for future ecological protection and restoration in similar ecologically fragile areas.

Heavy metals (HMs) or/and polycyclic aromatic hydrocarbons (PAHs) stress have significant adverse effects on the photosynthetic function and SPAD values of plants. Physiological integration is the typical feature of clonal plants, which can mitigate the adverse effects on ramets under heterogeneous stress. However, the sustainability of physiological integration between clones over prolonged stress durations, the dynamics of integration intensity and potential differences under various stress types remain unclear. This study examined the effects of three different heterogeneous stresses—cadmium (Cd), phenanthrene (Phe), and a combination of Cd and Phe (Cd + Phe) on the physiological integration of Zoysia japonica at different time points. The results indicate that physiological integration significantly enhances SPAD value, net photosynthetic rate (P_N), stomatal conductance (Cond), intercellular CO₂ concentration (C_i), transpiration rate (Tr), and water use efficiency (WUE). However, the physiological integration intensity diminishes with prolonged stress exposure. In addition, among different stress types, the initial integration intensity was highest under the highest toxicity conditions, it decreased most rapidly, resulting in the lowest integration intensity during the later stages of stress. To sum up, this study highlights the role of physiological integration in maintaining the photosynthetic function of clonal plants under heterogeneous stress and elucidates the temporal changes in integration intensity under different stress conditions.

Benzoapyrene (B[a]P) pollution poses a threat to the environment and the food chain and consequently to human health. However, the alleviation of the harmful effects of B[a]P pollution in perennial ryegrass (Lolium perenne L.) by the application of exogenous nitric oxide (NO) has been ignored. Thus, in this paper the effects of exogenous sodium nitroprusside (SNP, a NO donor) on the growth, photosynthetic fluorescence characteristics, and antioxidant enzyme activity of ryegrass exposed to B[a]P stress are investigated. B[a]P stress induced the reduction of the aboveground and belowground dry weights, chlorophyll (a, b), the total chlorophyll contents, the carotenoid content, the net photosynthetic rate (Pn), the intercellular carbon dioxide concentration (Ci), the water use efficiency (WUE), the photosystem II (PSII) potential activity (Fv/F0), the maximum quantum yield of PSII photochemistry (Fv/Fm), the steady-state fluorescence yield (Fs), and the non-photochemical quenching (qN), while enhancement was recorded in response to the foliar spray of SNP at 200 and 300 μmol·L-1 under B[a]P stress. Gray correlation and principal component analyses show that 200 μmol·L 1 of SNP more drastically alleviated the damage caused by B[a]P stress than 300 μmol·L 1 of SNP. The exogenous NO-mediated alleviation of B[a]P toxicity in ryegrass was associated with preserved photosynthetic characteristics and activation of antioxidant enzymes.