Many beneficial proteins have limited natural availability, which often restricts their supply and thereby reduces their potential for therapeutic or industrial usage. The advent of recombinant DNA (rDNA) technology enables the utilization of different microbes as surrogate hosts to facilitate the production of these proteins. This microbial technology continues to evolve and integrate with modern innovations to develop more effective approaches for increasing the production of recombinant biopharmaceuticals. These strategies encompass fermentation technology, metabolic engineering, the deployment of strong promoters, novel vector elements such as inducers and enhancers, protein tags, secretion signals, synthetic biology, high-throughput devices for cloning, and process screening. This appraisal commences with a general overview regarding the manufacture of recombinant proteins by microbes and the production of biopharmaceuticals, their trends towards the development of biopharmaceuticals, and then discusses the approaches adopted for accomplishing this. The design of the upstream process, which also involves host selection, vector design, and promoter design, is a crucial component of production strategies. On the other hand, the downstream process focuses on extraction and purification techniques. Additionally, the review covers the most modern tools and resources, methods for overcoming low expression, the cost of producing biopharmaceuticals in microbes, and readily available recombinant protein products.

Introduction: The decreasing level of physical activity among female undergraduates is worrying as it is associated with the increased risk of non-communicable diseases. Thus, this study aimed to identify the motivations, barriers and preferences towards exercise among female undergraduates in Malaysia.Methods: A non-probability purposive sampling was used for the recruitment process. The inclusion criteria of the participants were registered female undergraduates and aged between 18–30 years old. A semi-structured in-depth interview was used to collect topic-related information from the participants and signed consents were obtained prior to the interview. The interview questions were on respondents’ understanding of exercise, motivation and barriers to exercise, and exercise preferences. The recruitment process was conducted until the data was saturated. All interviews were audio recorded and manually transcribed verbatim. NVivo 11 was used to conduct the inductive analysis of the data to develop themes for motivation and barriers to exercise. For exercise preferences, four predetermined themes were used.Findings: A total of 26 respondents participated in this study. Eight themes were found for motivation to exercise, with the most common themes being maintaining or improving appearance, health benefits and togetherness. For barriers of exercising, five themes were found, and the most common ones were disliking exercise and no motivation. For exercise preferences, most respondents preferred a structured exercise program with flexibility in terms of when and where the exercise could be conducted. Light or moderate intensity exercise for 10–30 minutes with a frequency of 1–2 times a week was desired the most among the respondents.Conclusion: In conclusion, personal and environmental factors play important roles in motivating or hampering female undergraduates to exercise, and a structured program was the preferred mode of exercise of these respondents. A new exercise module was designed based on this needs assessment with a 70% acceptance rate among the participants. These findings can help the future development of more exercise modules tailored to female university students.

Introduction: For years, thousands of the Rohingya have fled Myanmar to reside in different states in Malaysia. Without any legal status, they have limited access to government medical and healthcare facilities. This is because Malaysia is not a State party to the 1951 Refugee Convention and its 1967 Protocol. IMAM Response and Relief Team (IMARET), a medical NGO, has organized charity clinics for this minority group to provide basic medical aids. Objectives: To identify common medical problems and medications prescribed during IMARET charity clinics in Kelantan. Methods: This was a cross-sectional study among Rohingya refugees attending IMARET charity clinics in Kelantan from June 2017 until May 2018. Data was collected from a medical clerking sheet. Medical problems were classified using ICD-10 classification, while medications prescribed were classified according to the first level of ATC code. Data were analysed using SPSS Version 24.0. Results: From 315 patients attended the clinic, most of the patients are of male gender (n=232, 73.1%). Mean age of the patients was 21.9 + 13.6 years (range: 1 – 75). Patients attended either one of the clinic locations; Tanah Merah (n=153, 48.5%), PengkalanChepa (n=78, 24.8%), and Kok Lanas (n=84, 26.6%). Most common medical problems observed were related to musculoskeletal system (n=104, 19.9%), mostly involving myalgia (n=96, 30.5%). Other common problems involved respiratory system (n=94, 18.0%), digestive system (n=68, 13.0%), and skin problems (n=57, 10.9%). Medications used for gastrointestinal disorders were mostly being prescribed in the clinics (n=179, 56.8%), followed by medications used for nervous system (n=114, 36.2%) and respiratory system (n=97, 30.8%). Most frequently prescribed medications include paracetamol, diclofenac, chlorpheniramine and multivitamins. Conclusion: Common medical problems observed among Rohingya refugees include myalgia, respiratory diseases and gastrointestinal problems. Therefore, medications used to treat these problems were shown to be frequently prescribed during clinics.International Journal of Human and Health Sciences Supplementary Issue: 2021 Page: S26

The Rohingya people are the most marginalized minorities in the world. Being female refugees in Malaysia, they are vulnerable to many challenges which may impact their menstrual hygiene. Currently, not much is known about their menstrual health practices and needs in Malaysia in which this knowledge gap needs to be addressed. This study aimed to identify the menstrual health practices and needs among Rohingya women refugees and determine the relationship between their socio-demographic profile and menstrual experiences. A cross sectional study involving 18 to 55 years old Rohingya women attending QFFD Clinic managed by IMARET in Selayang, Selangor between April to November 2022 was conducted. The MPQ and MPNS-36 were included practices and needs of Rohingya women in Malaysia. Nevertheless, they performed poorly in addressing their menstrual experiences. Thus, there is a need to include menstrual health humanitarian crisis