Tetrahydroquinoline (THQ) is an important structure for synthesizing multiple biologically active derivatives. Thus, we developed new quinoline derivatives and investigated them as anticancer agents. First, infrared spectroscopy, nuclear magnetic resonance spectroscopy, and other techniques were used to confirm the structure of synthesized compounds. Then, they were assessed in vitro against three human cancer cell lines. Consequently, four compounds, 10, 13, 15, and 16, were identified as promising anticancer agents with pyrazolo quinoline derivative (15) exhibiting the highest potential IC₅₀ and a strong apoptotic effect on three cell lines.

This study deals with the design and synthesis of new pyrazoline-thiazole scaffold as promising anti-cancer agents, with the goal of building new compounds with combinations of various heterocyclic moieties. The pyrazoline-thiazole scaffold was synthesized via cyclization of the chalcone derivatives with thiosemicarbazide to afford the corresponding thioamide 5, which was the key precursor for synthesis of the compounds 7-15. Three cancer cell lines (MCF-7, HepG-2 and A549) were used to examine in vitro anticancer activities of the recently synthesized compounds. Three compounds 7c, 9c, and 11d were found to show the most promising anti-cancer activity against three cell lines.