In the laboratory, I work on issues of wastewater and surface water microbiology, and more specifically on pathogens of faecal origin. Within the framework of research projects, I develop, as required, molecular biology methods for the detection and quantification of bacteria of interest such as Salmonella, Campilobacter or specific markers from animals digestive tract (goose, seagull and dog). The technologies we use range from culture on specific media, to amplification of specific DNA sequences by real-time quantitative PCR and digital PCR, enabling the detection of organisms at very low concentrations in samples. We also use sample-specific DNA extraction protocols to perform high-throughput sequencing for bacterial community analysis.