The aim of this study was to develop gelatin-based films incorporating Melaleuca alternifolia essential oil (MEO) and assess their application on refrigerated chicken breasts. The results showed that MEO exhibited antimicrobial activity against Pseudomonas aeruginosa and Salmonella sp., with inhibition zones of 17 mm and 9 mm, respectively. The minimum inhibitory concentration (MIC) was 10% for P. aeruginosa and 15% for Salmonella sp., demonstrating greater efficacy against P. aeruginosa. The antioxidant analysis using the ABTS method revealed activity of 1309 ± 18.0 μM Trolox/g, while the FRAP method resulted in 446 ± 5.78 μM FeSO₄/g. The characterization of the oil by gas chromatography identified major compounds, including 2-carene, γ-terpinene, terpine-4-ol, and α-terpineol. Incorporating the oil into gelatin films resulted in structural changes, such as an increase in thickness (from 0.059 to 0.127 mm) and water vapor permeability. Furthermore, the addition of MEO conferred homogeneous properties to the films with no visible cracks. The incorporation of MEO into gelatin films has shown ABTS antioxidant activity, and FRAP results showed a significant increase with higher MEO concentrations. The packaged samples retained more mass than the control group, which lost about 90% of its weight during storage. Texture analysis revealed only an 8% variation in the Melaleuca-coated samples compared to 19.6% in the control group. These findings indicate that gelatin films containing Melaleuca essential oil effectively improve the shelf life of chicken breasts.

This study investigated the use of aqueous pomegranate (Punica granatum L.) leaf extracts as a source of bioactive compounds in edible coatings for strawberry postharvest preservation. Extraction conditions were evaluated by varying solid-to-solvent ratio, temperature, and time, using total phenolic content (TPC) as the response variable. Response surface analysis indicated that the best predicted extraction conditions within the studied range were 1:50 (w/v), 57.36 °C, and 25 min. Among the evaluated treatments, extract C503 (1:50 (w/v), 50 °C, and 25 min) showed the highest experimental TPC (474.62 ± 21.69 mg GAE/g DM) and was selected for further characterization. This extract also showed high antioxidant capacity (FRAP: 7085 ± 72.0 µM FeSO₄/g; ABTS: 4921 ± 149.0 µM Trolox/g) and antimicrobial activity against Listeria monocytogenes and Staphylococcus aureus. When incorporated into gelatin- and starch-based edible coatings and applied to strawberries, both coatings reduced mass loss and delayed deterioration during nine days of storage at room temperature. At the end of storage, mass loss was reduced by approximately 25% with the gelatin-based coating and 11% with the starch-based coating. These results support aqueous pomegranate leaf extract as a promising source of bioactive compounds for the development of sustainable edible coatings to improve strawberry preservation.

This study investigates the effect of gamma irradiation on the physicochemical, mechanical, and antimicrobial properties of biodegradable composite films based on modified cassava starch/gelatin (GS) and wheat gluten/gelatin (GG), including their active formulations with 2% potassium sorbate (GS2S and GG2S, respectively). Films were produced by casting and irradiated at 2–32 kGy. Irradiation modulated the structure–property relationships of starch–protein matrices in a dose-dependent manner. In GG systems, tensile strength increased while elongation decreased, indicating enhanced intermolecular interactions. At higher doses (16–32 kGy), excessive rigidity was observed, whereas lower doses (2–8 kGy) provided a more favorable balance between strength and flexibility. Water vapor permeability decreased in selected formulations, while solubility results indicated the coexistence of crosslinking and chain scission mechanisms. Among the tested conditions, 2 kGy was identified as the optimal dose. Despite the incorporation of 2% potassium sorbate, antimicrobial performance remained limited, with modest inhibition against Wallemia sebi and negligible effects on Aspergillus chevalieri and Aspergillus montevidensis. HPLC analysis demonstrated a significant reduction in sorbate release after irradiation (up to ~80%), indicating that restricted mass transfer, rather than intrinsic antimicrobial inefficiency, governs the observed behavior. The main novelty of this work lies in demonstrating that irradiation-induced structural modifications improve mechanical and barrier properties but simultaneously hinder active compound release. This decoupling between structural performance and functional activity highlights a critical limitation in irradiated active films. Overall, gamma irradiation at 2 kGy is effective for tuning material properties; however, controlling release kinetics is essential to achieve functional antimicrobial performance in biodegradable active packaging systems.