Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has been detected in almost all organs of coronavirus disease-19 patients, although some organs do not express angiotensin-converting enzyme-2 (ACE2), a known receptor of SARS-CoV-2, implying the presence of alternative receptors and/or co-receptors. Here, we show that the ubiquitously distributed human transferrin receptor (TfR), which binds to diferric transferrin to traffic between membrane and endosome for the iron delivery cycle, can ACE2-independently mediate SARS-CoV-2 infection. Human, not mouse TfR, interacts with Spike protein with a high affinity (K _D ~2.95 nM) to mediate SARS-CoV-2 endocytosis. TfR knock-down (TfR-deficiency is lethal) and overexpression inhibit and promote SARS-CoV-2 infection, respectively. Humanized TfR expression enables SARS-CoV-2 infection in baby hamster kidney cells and C57 mice, which are known to be insusceptible to the virus infection. Soluble TfR, Tf, designed peptides blocking TfR-Spike interaction and anti-TfR antibody show significant anti-COVID-19 effects in cell and monkey models. Collectively, this report indicates that TfR is a receptor/co-receptor of SARS-CoV-2 mediating SARS-CoV-2 entry and infectivity by likely using the TfR trafficking pathway.

The purpose of this research was to determine the impact of seasonality on feed balance in the Lalo kile district of the Kellem Wollega Zone of Western Ethiopia. The district was divided into two agro-ecological zones: mid and low altitude. A total of 127 respondents were chosen to be interviewed. To collect the required data in the study areas, a cross-sectional survey was used. To analyses the qualitative data, descriptive statistics were performed, while the General Linear Model (GLM) method was used to assess the impacts of seasonal feed availability and agro-ecology on dry matter yield and production constraints. The result showed that there has been seasonal fluctuation in the feed availability and the feed is generally abundant from June to September. The feed supply in terms of DM, ME, and DCP per household per year was 118.7 kg, 74,781 MJ/kg, and 118.61kg, respectively. The feed balance was negatively associated and substantially different all across the studied areas (P < 0.01). With a year's approach, the annual total feed supply exceeds only 66.13, 25.81, and 87.24 percent of the DM, DCP, and ME demands per household, respectively. As a result, total dry matter production, digestible crude protein, and metabolizable energy supply were only fulfilled for 9, 3.5, and 12 months in the research area's mid-altitude and 7, 3 and 9 months in the study area's low land altitude, respectively. The main limitations in both agro-ecologies of the research area include low production and reproduction, poor use of existing feedstuffs, and poor adoption of improved forage production. As a result, for successful livestock development in the study area, the adoption of better fodder and supplementation of productive animals should be considered.