Tingting Wang

Dr. Tingting Wang

orcid

Research Focus & Metrics

Academic Career Length

8Years (2019-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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29 March 2021 in eLife
Verified
Journal Article
Open access
Tingting WangTingting Wang
Tingting Wang
WSWenju Sun
Wenju Sun
LFLigang Fan
Ligang Fan
CHCanfeng Hua
Canfeng Hua
NWNan Wu
Nan Wu
JYJian Yan
Jian Yan
A high-throughput systematic evolution of ligands by exponential enrichment assay was applied to 371 putative TFs in Pseudomonas aeruginosa, which resulted in the robust enrichment of 199 unique sequence motifs describing the binding specificities of 182 TFs. By scanning the genome, we predicted in total 33,709 significant interactions between TFs and their target loci, which were more than 11-fold enriched in the intergenic regions but depleted in the gene body regions. To further explore and delineate the physiological and pathogenic roles of TFs in P. aeruginosa, we constructed regulatory networks for nine major virulence-associated pathways and found that 51 TFs were potentially significantly associated with these virulence pathways, 32 of which had not been characterized before, and some were even involved in multiple pathways. These results will significantly facilitate future studies on transcriptional regulation in P. aeruginosa and other relevant pathogens, and accelerate to discover effective treatment and prevention strategies for the associated infectious diseases.

2 October 2020 in Nature Communications
Verified
Journal Article
Open access
PDF file
LFLigang Fan
Ligang Fan
Tingting WangTingting Wang
Tingting Wang
CHCanfeng Hua
Canfeng Hua
WSWenju Sun
Wenju Sun
XLXiaoyu Li
Xiaoyu Li
XDXin Deng
Xin Deng
Pseudomonas syringae is a Gram-negative and model pathogenic bacterium that causes plant diseases worldwide. Here, we set out to identify binding motifs for all 301 annotated transcription factors (TFs) of P. syringae using HT-SELEX. We successfully identify binding motifs for 100 TFs. We map functional interactions between the TFs and their targets in virulence-associated pathways, and validate many of these interactions and functions using additional methods such as ChIP-seq, electrophoretic mobility shift assay (EMSA), RT-qPCR, and reporter assays. Our work identifies 25 virulence-associated master regulators, 14 of which had not been characterized as TFs before.

15 May 2019 in Applied and Environmental Microbiology
Verified
Journal Article
Open access
PDF file
Tingting WangTingting Wang
Tingting Wang
ZCZhao Cai
Zhao Cai
XSXiaolong Shao
Xiaolong Shao
WZWeitong Zhang
Weitong Zhang
YXYingpeng Xie
Yingpeng Xie
XDXin Deng
Xin Deng
The present work comprehensively analyzed the transcriptome and phenotypes that were regulated by c-di-GMP in P. syringae . Given that the majority of diguanylate cyclases and phosphodiesterases have not been characterized in P. syringae , this work provided a very useful database for the future study on regulatory mechanism (especially its relationship with T3SS) of c-di-GMP in P. syringae . In particular, we identified three promoters that were sensitive to elevated c-di-GMP levels and inserted them into luciferase-based reporters that effectively respond to intracellular levels of c-di-GMP in P. syringae , which could be used as an economic and efficient way to measure relative c-di-GMP levels in vivo in the future.