This paper aimed to investigate the potential antifungal influences of new alkaloids from Delphinium peregrinum L. var. eriocarpum Boiss. New Diterpenoid alkaloids Delcarpum (1), Hydrodavisine (4) and known alkaloids Peregrine (2), Delphitisine (3) were isolated by different chromatographic methods from the aerial parts of D. Peregrinum eriocarpum Boiss, which grows in Syria. The structures of alkaloids were proposed based on 1D NMR spectroscopy ¹H-NMR, ¹³C-NMR, DEPT-135, DEPT-90, 2D NMR spectroscopy DQF-COSY, HMQC, EI-Ms mass spectrum, and IR spectroscopic measurements. The antifungal activity of the isolated alkaloids was evaluated against different dermatophyte fungal isolates compared with fluconazole. In the case of Peregrine (2) the minimum inhibitory concentrations(MICs) recorded 128–256, 32–64, and 32 for Epidermophyton floccosum, Microsporum canis, and Trichophyton rubrum, respectively, compared to 32–64, 16, and 32 μg/mL in the case of fluconazole, respectively. The MICs recorded on application of the four alkaloids mixture were 64, 32, and 16 in the case of E. floccosum, M. canis, and T. rubrum, respectively, which were significantly lower than that measured for each of the individual alkaloid and were compatible for fluconazole. In conclusion, MICs of the tested alkaloids showed a variable potential effect on the investigated fungal isolates. Peregrine (2) was the most effective alkaloid, however, the application of the mixture of alkaloids induced significant synergistic activity that was more pronounced than the application of individual ones.

The study describes the effect of vanillin derivative on the activity of aryl esterase in vitro. After that, the Estimated effectiveness of the enzyme aryl esterase, troponin T, and lipids. The research included the collection of 120 blood samples distributed to 60 blood samples of healthy people as a control group and 60 blood samples of patients with atherosclerosis who were diagnosed under the supervision of specialized doctors at Salah Eddin General Hospital / Catheterization Unit. Results showed that the vanillin derivative has an invigorating effect on the activity of aryl esterase, and that the degree of enzyme activation increases with the increase in the concentration of the derivative, while there was lower with a probability level (P≤ 0.0006) in the activity of aryl esterase from the serum of patients with atherosclerosis in addition, a significant increase at the probability level ( P ≤ 0.001) in the level of total cholesterol, triglycerides, LDL-C and VLDL-C, while there was decrease at the probability level (P ≤ 0.0004) in the level of HDL-C. Keywords: Aryl esterase, Vanillin derivative, Lipids, Atherosclerosis.