The objective was to determine the forage production and nutritional quality of six buffel grass ecotypes in vegetative and physiological maturity stages. The experiment was carried out at the Los Cañones Experimental Station in Zacatecas, Mexico. The ecotypes were as follows: E-42, E-45, E-66, E-72, E-S245, and the Titan variety as a control. The experiment design was a completely randomized block with three replications, and the evaluation years were from 2020 to 2022. The variables measured were as follows: plant height (PH), dry matter yield (DMY), leaf (Lf) and stem-inflorescence (St), crude protein (CP), neutral (NDF), and acid detergent fiber (ADF). Pearson’s correlation and a combined analysis of variance were performed for each growth stage. A correlation analysis showed significant relationships (p < 0.05) between variables. In the vegetative stage, ecotypes E-42, E-45, and E-S45 showed accumulations of over 12% of CP, which were higher (p < 0.05) than the ones for Titan. Low values of E-S245 (p < 0.05) for NDF (63.44%) and ADF (44.49%) stood out among all ecotypes. In the maturity stage, Titan was surpassed by E-45 and E-S245 with CP higher than 4%. The neutral and acid fibers in E-42, E-45, and E-S245 were less than in Titan (p < 0.05). Ecotypes E-42 and E-S245 are alternatives to improve feed efficiency in the dry subtropical climate of Zacatecas.