Background. The present study aimed to examine the psychometric properties of the Lithuanian adaptation of the TEIQue-SF. Methods. The analyses were performed using a sample of 199 preschool teachers (100% women; mean age = 46.70, SD = 11.70 years, age range = 21–69 years) from across Lithuania. The Teacher Subjective Wellbeing Questionnaire was also administered as an external validation measure. The reliability of the TEIQue-SF was assessed by Cronbach’s α and McDonald’s ω. Finally, we examined the relationship between sociodemographic characteristics and global trait emotional intelligence. Results. Confirmatory factor analysis of the Lithuanian TEIQue-SF supported the one-factor structure of trait emotional intelligence (CFI = 0.99, TLI = 0.98, IFI = 0.99, RMSEA = 0.04, SRMR = 0.02). Good internal consistency was observed for global trait emotional intelligence (α = 0.85, ω = 0.84). Global trait emotional intelligence was significantly and positively associated with the teacher general well-being (β = 0.28), school connectedness (β = 0.26), and teaching efficacy (β = 0.28). Age was the only sociodemographic indicator positively related to global trait EI (β = 0.26). Conclusions. Our research showed that the Lithuanian version of the TEIQue-SF is a valid and reliable instrument to measure trait emotional intelligence and can be recommended for research and practical use.