To promote antimicrobial stewardship and prevent an epidemic of antimicrobial-resistant bacteria, I address the following research themes: 1) Surveillance of antimicrobial-resistant bacteria We investigate the molecular epidemiology of the latest clinical isolates of Staphylococcus aureus, Streptococcus pyogenes, and Nontuberculous Mycobacteria. 2) Elucidation of virulence mechanisms We identify bacterial virulence factors that contribute to severe infectious diseases using 3D skin and lung tissue models and murine infection models. 3) Bacteria associated with acne pathology The antimicrobial resistance and exacerbating factors of Cutibacterium acnes, which is isolated from patients with acne vulgaris, are characterized. 4) Development of anti-infection drugs Alternative therapies, such as anti-virulence agents that modulate the production of bacterial toxins or virulence factors without exhibiting antimicrobial activity, are explored.