Lutein is particularly known to help maintain normal visual function by absorbing and attenuating the blue light that strikes the retina in our eyes. The effect of overexposure to blue light on our eyes due to the excessive use of electronic devices is becoming an issue of modern society due to insufficient dietary lutein consumption through our normal diet. There has, therefore, been an increasing demand for lutein-containing dietary supplements and also in the food industry for lutein supplementation in bakery products, infant formulas, dairy products, carbonated drinks, energy drinks, and juice concentrates. Although synthetic carotenoid dominates the market, there is a need for environmentally sustainable carotenoids including lutein production pathways to match increasing consumer demand for natural alternatives. Currently, marigold flowers are the predominant natural source of lutein. Microalgae can be a competitive sustainable alternative, which have higher growth rates and do not require arable land and/or a growth season. Currently, there is no commercial production of lutein from microalgae, even though astaxanthin and β-carotene are commercially produced from specific microalgal strains. This review discusses the potential microalgae strains for commercial lutein production, appropriate cultivation strategies, and the challenges associated with realising a commercial market share.

In this study, FeSO₄ supplementation ranging from 0 to 4.5 mM, and MgSO₄ supplementation ranging from 0 to 5.1 mM were investigated to observe the effect on the population dynamics, biochemical composition and fatty acid content of mixed microalgae grown in Anaerobic Liquid Digestate (ALD). Overall, 3.1 mM FeSO₄ addition into ALD increased the total protein content 60% and led to highest biomass (1.56 g L⁻¹) and chlorophyll-a amount (18.7 mg L⁻¹) produced. Meanwhile, 0.4 mM MgSO₄ addition increased the total carotenoid amount 2.2 folds and slightly increased the biomass amount. According to the microbial community analysis, Diphylleia rotans, Synechocystis PCC-6803 and Chlorella sorokiniana were identified as mostly detected species after confirmation with 4 different markers. The abundance of Chlorella sorokiniana and Synechocystis PCC-6803 increased almost 2 folds both in iron and magnesium addition. On the other hand, the dominancy of Diphylleia rotans was not affected by iron addition while drastically decreased (95%) with magnesium addition. This study helps to understand how the dynamics of symbiotic life changes if macro elements are added to the ALD and reveal that microalgae can adapt to adverse environmental conditions by fostering the diversity with a positive effect on high value product.

Understanding how to address today’s global challenges is critical to improving corporate performance in terms of economic and environmental sustainability. In wastewater treatment systems, such an approach implies integrating efficient treatment technologies with aspects of the circular economy. In this business field, energy costs represent a large share of operating costs. This work discusses technological and management aspects leading to greater energy savings in Portuguese wastewater treatment companies. A mixed methodology, involving qualitative and quantitative aspects, for collecting and analysing data from wastewater treatment plants was used. The qualitative aspects consisted of a narrative analysis of the information available on reports and websites for 11 wastewater management companies in Portugal (e.g., technologies, treated wastewater volumes and operating costs) followed by a review of several international studies. The quantitative approach involved calculating the specific energy consumption (kWh/m³), energy operating costs (EUR/m³) and energy operating costs per population equivalent (EUR/inhabitants) using data from the literature and from Portuguese companies collected from the SABI database. The results suggested that the most environmentally and economically sustainable solution is algae-based technology which might allow a reduction in energy operating costs between 0.05–0.41 EUR/m³ and 15.4–180.8 EUR/inhabitants compared to activated sludge and other conventional methods. This technology, in addition to being financially advantageous, provides the ability to eliminate the carbon footprint and the valorisation of algae biomass, suggesting that this biotechnology is starting to position itself as a mandatory future solution in the wastewater treatment sector.

Economizing microalgal cultivation is a considerable milestone targeted by efforts put into microalgal biorefineries. In light of that, the present study was aimed to explore the potential of using anaerobic liquid digestate (ALD) as culture media to grow microalgae and compared it with three different synthetic media (i.e., N8, BBM, and M8) in terms of biomass yield, fatty acid composition, and nutrient utilization/recovery. Moreover, a mixed culture of wild-type microalgae was employed in this study owing to the ability of mixed cultures to survive extreme conditions, eliminating the risk of losing the culture easily, as it mostly happens with pure cultures. The highest nutrient yield coefficients were achieved when the mixed microalgae culture was cultivated in ALD, where the yield coefficient for nitrogen (YN) and yield coefficient for phosphorus (YP) were 10.7 mg biomass mg-1 N and 98 mg biomass mg-1 P, respectively. The highest lipid content (34%) and the highest concentrations of C16:0 (114 mg L-1) and C18:0 (60.9 mg L-1) were also recorded when the mixed microalgae culture was cultivated in ALD. Furthermore, the polyunsaturated fatty acids (PUFA) content also increased significantly in ALD, a beneficial phenomenon as PUFAs in microalgae allow them to adapt more effectively to extreme conditions. Based on the microbial community analysis performed using the multi-marker metabarcoding approach, Diphylleia rotans, Synechocystis PCC-6803, Cyanobium gracile PCC 6307, and Chlorella sorokiniana were identified as the most abundant species in the ALD growth. Overall, based on the findings of the present study, ALD could be used as a promising cultivation medium for microalgae, offering a process integration approach to combine anaerobic digestion and algae cultivation as an effective way to simultaneously treat the high-strength dark-colored ALD and valorize it into profitable byproducts.