Dr. Rubbo has investigated the biological production of nitric oxide (•NO) and reactive oxygen species and its central role in the pathogenesis of acute and chronic diseases. His independent research on the biological chemistry and pharmacology of •NO has been critically contributed to unravel new and pathophysiologically relevant information about the reactions of •NO and oxidized lipids and demonstrated the products of this reaction to be important mediators of tissue oxidant protection. Investigations were particularly focused on the antioxidant roles that •NO plays at the biochemical and cellular levels. Understanding the reactions of •NO-derived reactive species with unsaturated fatty acids forming nitro-fatty acids (nitroalkenes) has been a central focus of Rubbo’s research unifies eicosanoid and •NO cell signaling pathways so critical for the regulation of tissue and inflammatory function. Key findings from his research include the clinical detection of nitrated lipids in human plasma that increases during inflammatory conditions. The lab is performing lipidomic analysis of ALS patients with the aim to discover new molecules for diagnosis and treatment. His studies provide a platform for developing novel pharmacologic interventional strategies directed towards modulating inflammatory tissue injury. He has published over eighty original research and high-cited review articles (H Index 35).