Rapid antigen tests are currently used for population screening of COVID-19. However, they lack sensitivity and utilize antibodies as receptors, which can only function in narrow temperature and pH ranges. Consequently, molecularly imprinted polymer nanoparticles (nanoMIPs) are synthetized with a fast (2 h) and scalable process using merely a tiny SARS-CoV-2 fragment (∼10 amino acids). The nanoMIPs rival the affinity of SARS-CoV-2 antibodies under standard testing conditions and surpass them at elevated temperatures or in acidic media. Therefore, nanoMIP sensors possess clear advantages over antibody-based assays as they can function in various challenging media. A thermal assay is developed with nanoMIPs electrografted onto screen-printed electrodes to accurately quantify SARS-CoV-2 antigens. Heat transfer-based measurements demonstrate superior detection limits compared to commercial rapid antigen tests and most antigen tests from the literature for both the alpha (∼9.9 fg mL^–1) and delta (∼6.1 fg mL^–1) variants of the spike protein. A prototype assay is developed, which can rapidly (∼15 min) validate clinical patient samples with excellent sensitivity and specificity. The straightforward epitope imprinting method and high robustness of nanoMIPs produce a SARS-CoV-2 sensor with significant commercial potential for population screening, in addition to the possibility of measurements in diagnostically challenging environments.

There has been a rapid increase in the fabrication of various kinds of edible nanoparticles for oral delivery of bioactive agents, such as those constructed from proteins, carbohydrates, lipids, and/or minerals. It is currently difficult to compare the relative advantages and disadvantages of different kinds of nanoparticle-based delivery systems because researchers use different analytical instruments and protocols to characterize them. In this paper, we briefly review the various analytical methods available for characterizing the properties of edible nanoparticles, such as composition, morphology, size, charge, physical state, and stability. This information is then used to propose a number of standardized protocols for characterizing nanoparticle properties, for evaluating their stability to environmental stresses, and for predicting their biological fate. Implementation of these protocols would facilitate comparison of the performance of nanoparticles under standardized conditions, which would facilitate the rational selection of nanoparticle-based delivery systems for different applications in the food, health care, and pharmaceutical industries.

Prostate cancer (PCa) is the second most common male cancer and is attributable to over 375,000 deaths annually. Prostate specific antigen (PSA) is a key biomarker for PCa and therefore measuring patient PSA levels is an important aspect of the diagnostic pathway. Automated immunoassays are currently utilized for PSA analysis, but they require a laboratory setting with specialized equipment and trained personnel. This results in high diagnostic costs, extended therapeutic turnaround times, and restrictions on testing capabilities in resource-limited settings. Consequently, there is a strong drive to develop point-of-care (PoC) PSA tests that can offer accurate, low-cost, and rapid results at the time and place of the patient. However, many emerging PoC tests experience a trade-off between accuracy, affordability, and accessibility which distinctly limits their translational potential. This review comprehensively assesses the translational advantages and limitations of emerging laboratory-level and commercial PoC tests for PSA determination. Electrochemical and optical PSA sensors from 2013 to 2023 are systematically examined. Furthermore, we suggest how the translational potential of emerging tests can be optimized to achieve clinical implementation and thus improve PCa diagnosis globally.

This paper provides evidence and discusses the variability in the thermomechanical behaviour of virgin and recycled polypropylene/high-density polyethylene blends without the addition of other components, which is sparse in the literature. Understanding the performance variability in recycled polymer blends is of critical importance in order to facilitate the re-entering of recycled materials to the consumer market and, thus, contribute towards a circular economy. This is an area that requires further research due to the inhomogeneity of recycled materials. Therefore, the thermal and mechanical properties of virgin and recycled polypropylene/high-density polyethylene blends were investigated systematically. Differential scanning calorimetry concludes that both the recycled and virgin blends are immiscible. Generally, recycled blends have lower overall crystallinity and melting temperatures compared with virgin blends while, remarkably, their crystallisation temperatures are compared favourably. Dynamical mechanical analysis showed little variation in the storage modulus of recycled and virgin blends. However, the alpha and beta relaxation temperatures are lower in recycled blends due to structural deterioration. Deterioration in the thermal and mechanical properties of recycled blends is thought to be caused by the presence of contaminants and structural degradation during reprocessing, resulting in shorter polymeric chains and the formation of imperfect crystallites. The tensile properties of recycled blends are also affected by the recycling process. The Young’s modulus and yield strength of the recycled blends are inferior to those of virgin blends due to the deterioration during the recycling process. However, the elongation at break of the recycled blends is higher compared with the virgin blends, possibly due to the plasticity effect of the low-molecular-weight chain fragments.