Bumble bees and honey bees are of vital importance for tomato pollination, although honey bees are less attracted to tomato flowers than bumble bees. Little is known about how tomato flower volatile compounds influence the foraging behaviors of honey bees and bumble bees. In this study, compounds of tomato flower volatiles were detected by gas chromatography–mass spectrometry. Electroantennography (EAG) and a dynamic two-choice olfactometer were used, respectively, to compare the differences of antennal and behavioral responses between Apis mellifera and Bombus terrestris towards selected volatile compounds. A total of 46 compounds were detected from the tomato flower volatiles. Of the 16 compounds tested, A. mellifera showed strong antennal responses to 3 compounds (1-nonanal, (+)-dihydrocarvone, and toluene) when compared with a mineral oil control, and B. terrestris showed 7 pronounced EAG responses (1,3-xylene, (+)-dihydrocarvone, toluene, piperitone, eucarvone, 1-nonanal, and β-ocimene). Additionally, 1-nonanal and (+)-dihydrocarvone elicited significant avoidance behavior of A. mellifera, but not of B. terrestris. In conclusion, bumble bees are more sensitive to the compounds of tomato flower volatiles compared to honey bees, and honey bees showed aversion to some compounds of tomato flower volatiles. The findings indicated that compounds of flower volatiles significantly influenced bee foraging preference for tomato.

As important pollinators, honeybees and bumblebees present a pollination behavior that is influenced by flower volatiles through the olfactory system. In this study, volatile compounds from melon flowers were isolated and identified by headspace solid-phase microextraction (HS-SPME) and gas chromatography–mass spectrometry (GC-MS), and their effects on Apis mellifera and Bombus terrestris were investigated by electroantennogram (EAG) and behavior tests (Y-tube olfactometer). The results showed that 77 volatile compounds were detected in melon flowers, among which the relative content of aldehydes was the highest (61.34%; 82.09%). A. mellifera showed a strong EAG response to e-2-hexenal, e-2-octenal, and 1-nonanal. B. terrestris showed a strong EAG response to e-2-hexenal, e-2-octenal, 2,5-dimethyl-benzaldehyde, benzaldehyde and benzenepropanal. In behavior tests, the volatiles with the highest attractive rate to A. mellifera were e-2-hexenal (200 μg/μL, 33.33%) and e-2-octenal (300 μg/μL, 33.33%), and those to B. terrestris were e-2-hexenal (10 μg/μL, 53.33%) and 2,5-dimethyl-benzaldehyde (100 μg/μL, 43.33%). E-2-hexenal and e-2-octenal were more attractive to A. mellifera than B. terrestris, respectively (10 μg/μL, 10 μg/μL, 200 μg/μL). In conclusion, the volatiles of melon flowers in facilities have certain effects on the electrophysiology and behavior of bees, which is expected to provide theoretical and technical support for the pollination of A. mellifera and B. terrestris in facilities.

Honeybee olfaction can influence foraging behavior and affect crop pollination. Odor-binding proteins play a vital role in honeybee olfactory perception. A previous study based on the antennal transcriptome of Apis mellifera ligustica in melon and tomato greenhouses revealed that AmelOBP2 is highly expressed. Therefore, we aimed to further investigate the olfactory recognition mechanism of honeybees by detecting the expression levels and binding ability of AmelOBP2 to floral volatiles of melon and tomato flowers. The results show that AmelOBP2 mRNA was highly expressed in the antennae of honeybees, and its protein expression was highest in the antennae at 20 days of age and was higher in the melon greenhouse. The binding ability of AmelOBP2 to floral volatiles of melon was stronger than that of tomato. AmelOBP2 had a stronger binding ability with aldehydes in melon floral volatiles and with terpenes and benzenes in tomato floral volatiles. After feeding with siRNA, the electroantennogram response of honeybees to E-2-hexenal, E-2-octenal, and 1-nonanal decreased markedly, confirming the role of AmelOBP2 in the recognition of melon and tomato floral volatiles. These results elucidate the molecular mechanisms underlying honeybee flower-visiting behavior and provide a theoretical reference for regulating the behavior of honeybees using plant volatiles.