Spectralis optical coherence tomography (OCT) provided more detailed parameters in the peripapillary and macular areas among the OCT machines, but it is not easy to understand the enormous information (114 features) generated from Spectralis OCT in glaucoma assessment. Machine learning methodology has been well-applied in glaucoma detection in recent years and has the ability to process a large amount of information at once. Here we aimed to analyze the diagnostic capability of Spectralis OCT parameters on glaucoma detection using Support Vector Machine (SVM) classification method in our population. Our results showed that applying all OCT features with the SVM method had good capability in the detection of glaucomatous eyes (area under curve (AUC) = 0.82), as well as discriminating normal eyes from early, moderate, or severe glaucomatous eyes (AUC = 0.78, 0.89, and 0.93, respectively). Apart from using all OCT features, the minimum rim width (MRW) may be good feature groups to discriminate early glaucomatous from normal eyes (AUC = 0.78). The combination of peripapillary and macular parameters, including MRW_temporal inferior (TI), MRW_global (G), ganglion cell layer (GCL)_outer temporal (T2), GCL_inner inferior (I1), peripapillary nerve fiber layer thickness (ppNFLT)_temporal superior (TS), and GCL_inner temporal (T1), provided better results (AUC = 0.84). This study showed promise in glaucoma management in the Taiwanese population. However, further validation study is needed to test the performance of our proposed model in the real world.