Chronic kidney disease (CKD) is directly influenced by the deleterious effects of systemic inflammation and oxidative stress. The vascular endothelium may transiently respond to aerobic exercise and improve post-exercise vascular renal function in moderate stages of CKD. Brachial artery flow-mediated dilation (FMD) is a nitric-oxide-dependent measure of endothelial function that is transiently potentiated by exercise. The purpose of the study was to determine the acute influence of a single bout of high-intensity interval exercise (HIIE) or steady-state moderate-intensity exercise (SSE) on endothelial dysfunction in moderate stages of CKD. Twenty participants (n = 6 men; n = 14 women) completed 30 min of SSE (65%) and HIIE (90:20%) of VO_2reserve in a randomized crossover design. FMD measurements and blood samples were obtained before, 1 h, and 24 h post-exercise. FMD responses were augmented 1 h post-exercise in both conditions (p < 0.005). Relative to pre-exercise measures, total antioxidant capacity increased by 4.3% 24 h post-exercise (p = 0.012), while paraoxonase-1 was maintained 1 h and elevated by 6.1% 24 h after SSE, but not HIIE (p = 0.035). In summary, FMD can be augmented by a single episode of either HIIE or SSE in moderate stages of CKD. Modest improvements were observed in antioxidant analytes, and markers of oxidative stress were blunted in response to either SSE or HIIE.

Our purpose was to characterize changes in paraoxonase 1 (PON1) activity and concentration after single aerobic exercise sessions conducted before and after 6 weeks of niacin therapy in men with metabolic syndrome (MetS). Twelve men with MetS expended 500 kcal by walking at 65% of VO2max before and after a 6-week regimen of niacin. Niacin doses were titrated by 500 mg/week from 500 to 1500 mg/day and maintained at 1500 mg/day for the last 4 weeks. Fasting blood samples were collected before and 24 hours after each exercise session and analyzed for PON1 activity, PON1 concentration, myeloperoxidase (MPO), apolipoprotein A1, oxidized low-density lipoprotein (oLDL), lipoprotein particle sizes and concentrations. PON1 activity, PON1 concentration, MPO, and oLDL were unaltered following the independent effects of exercise and niacin (P > 0.05 for all). High-density lipoprotein particle size decreased by 3% (P = 0.040) and concentrations of small very low-density lipoprotein increased (P = 0.016) following exercise. PON1 activity increased 6.1% (P = 0.037) and PON1 concentrations increased 11.3% (P = 0.015) with the combination of exercise and niacin. Exercise and niacin works synergistically to increase PON1 activity and concentration with little or no changes in lipoproteins or markers of lipid oxidation.

An observational study was conducted to evaluate hygiene habits of students with fields of study, gender, and understanding of hygiene at a university in Alabama. One hundred students were randomly observed in ten restrooms on campus to determine whether or not students washed their hands. The study was divided into an observational stage, a quiz to ascertain student9s knowledge of hygiene and the spread of pathogens, and a survey of self-reported illness rates. Females had a tendency to wash their hands more often than males while visiting the bathroom (p = 0.02, X² = 11.6). Science majors were more likely to wash their hands than non-science majors (p ≤ 0.001, X² = 5.2). Females (p ≤ 0.0001, df = 98, F = 21.5) and science majors (p ≤ 0.0001, df = 98, F = 81.4) scored significantly higher on the survey than males and non-science majors, and that those observed not washing their hands reported being sick more often than those observed washing their hands (X² = 155.0, df = 3, p < 0.001, Fisher9s exact p< 0.001).

Purpose: Efficacy of exercise to improve renal health and filtration remains understudied in adults with moderate-stages (stages G3a-b) of chronic kidney disease (CKD). Acute exercise may contribute clinically relevant information for exercise-related augmentation of renal health and filtration in CKD. Urine epidermal growth factor (uEGF) and cystatin C (CyC) are proposed to be more direct biomarkers of renal health and filtration. This study aimed to determine the influence of continuous moderate-intensity exercise (CMIE) and high-intensity interval exercise (HIIE) on traditional and novel biomarkers of renal health and filtration in moderate-stages of CKD. Methods: Twenty CKD participants completed 30 minutes of both CMIE and HIIE. Blood and urine samples were obtained pre, 1-hour, and 24-hours post-exercise. Traditional—serum creatinine (sCr) urine creatinine, novel—uEGF, uEGF ratio (uEGFr), and CyC. Estimates of glomerular filtration rate (eGFR)—modification of diet in renal disease (MDRD) and the CKD-Epidemiology (CKD-EPI)—responses were compared pre, 1 hr, and 24 hr post-exercise. Results: Relative to pre-exercise measures, uEGF remained unchanged in both exercise conditions. However, uEGFr was 5.4% greater 24-hours after HIIE (P = .05), while uEGFr remained unchanged with CMIE. sCr decreased 6 to 19% 1-hour post-exercise in both conditions (P = .009). On average renal filtration increased in eGFR-MDRD (7.2 ± 2.0 ml/min/1.73 m²) (P = .007) and eGFR-CKD-EPI (8.6 ± 2.3 ml/min/1.73 m²) 1-hour post-exercise (P = .009). Conclusion: By clinical estimates, renal filtration in CKD was not normalized but transiently improved regardless of exercise condition, with HIIE eliciting transient improvements in renal health.

Purpose: To determine the acute and chronic effects of exercise on Paraoxonase-1 (PON1) concentration and activity. Methods: A literature search was performed using 16 electronic databases. Effect sizes (ES) were computed and two-tailed α values < .05 and non-overlapping 95% confidence intervals (95%CI) were considered statistically significant. Heterogeneity, inconsistency (I²), and small-study effects using the LFK index were examined. Results: Eighteen studies (n = 377 participants) met the criteria for inclusion. The acute effects of exercise on PON1 concentration were trivial and non-significant (ES = −.03, 95%CI = −.39 to .34, p > .05), heterogeneous (p = .05), moderately inconsistent (I² = 48%), with minor asymmetry (LFK index = 1.34). The chronic effects of exercise on PON1 concentration were also trivial and non-significant (ES = −.04, 95%CI = −.53 to.45, p > .05), homogenous (p = .65), displayed low inconsistency (I² = 0%), and minor asymmetry (LFK index = −1.14). The acute effects of exercise on PON1 activity were trivial and non-significant (ES = .11, 95%CI = −.02 to.24, p > .05), homogenous (p = .85), showed low inconsistency (I² = 0%), and no asymmetry (LFK index = .82). The chronic effects of exercise on PON1 activity were trivial and non-significant (ES = .31, 95%CI = −.03 to.65, p > .05), homogenous (p = .17), moderately inconsistent (I² = 36%), with no asymmetry (LFK index = .60). Conclusion: Acute and chronic exercise training, overall, exerted a trivial effect on PON1 concentration and activity.

This study examined the effects of two different acute bouts of treadmill running (e.g., steady-state moderate-intensity exercise (SSE) and high-intensity interval exercise (HIIE)) on adipokine (e.g., adiponectin, leptin, and omentin) concentrations in individuals with moderate stages of chronic kidney disease (CKD). Fourteen participants (8 females and 6 males) (age 58.7 ± 9.7, height (cm) 168.5 ± 9.7, weight (kg) 83.5 ± 18.2) were classified as having moderate stages of CKD (stages G3a and G3b; estimated glomerular filtration rate (eGFR) between 59 and 30 mL/min/1.73 m²). Participants completed 30 min of either SSE at 65% oxygen consumption reserve (VO₂R) or HIIE for 3 min at a 90% VO₂R separated by 2 min of slow walking (20% VO₂R) in a randomized, crossover design on a treadmill. Venous blood samples were obtained at baseline, 1 h, and 24 h post exercise. Data were analyzed using a two by three repeated measures ANOVA (p < 0.05). There were no significant differences in adiponectin (p < 0.353), leptin (p < 0.084), and omentin (p < 0.235) concentrations between SSE and HIIE. Similarly, no significant differences were seen between the sampled time points for either aerobic exercise condition. In conclusion, this study demonstrated there were no changes in adiponectin, leptin, or omentin concentrations when performing an acute bout of HIIE or SSE for 30 min, at 1 or 24 h post exercise. Future studies should seek to either increase the duration of the exercise bout or identify different adipokines to examine for patients experiencing moderate stages of CKD.