Ca²⁺ channels are essential to cell birth, life, and death. They can be externally activated by optogenetic tools, but this requires robust introduction of exogenous optogenetic genes for expression of photosensitive proteins in biological systems. Here we present femtoSOC, a method for direct control of Ca²⁺ channels solely by ultrafast laser without the need for optogenetic tools or any other exogenous reagents. Specifically, by focusing and scanning wavelength-tuned low-power femtosecond laser pulses on the plasma membrane for multiphoton excitation, we directly induced Ca²⁺ influx in cultured cells. Mechanistic study reveals that photoexcited flavins covalently bind cysteine residues in Orai1 via thioether bonds, which facilitates Orai1 polymerization to form store-operated calcium channels (SOCs) independently of STIM1, a protein generally participating in SOC formation, enabling all-optical activation of Ca²⁺ influx and downstream signaling pathways. Moreover, we used femtoSOC to demonstrate direct neural activation both in brain slices in vitro and in intact brains of living mice in vivo in a spatiotemporal-specific manner, indicating potential utility of femtoSOC.

The present study aimed to examine whether intellectually gifted children had better planning skills than their chronological-age controls and what processing skills may explain these differences. A total of 35 intellectually gifted Chinese children (25 boys and 10 girls; M_age = 12.77 years) and 39 chronological-age controls (27 boys and 12 girls; M_age = 12.89 years) participated in this study. They were assessed on three measures of operational planning (Planned Codes, Planned Connections, and Planned Search), on a measure of action planning (Crack the Code), and on measures of processing speed, working memory, and attention. Results of analysis of variance (ANOVA) showed first that the two groups differed in Crack the Code (accuracy and first move time) and in Planned Connections. Whereas processing speed explained the group differences in Planned Connections, none of the processing skills were able to eliminate the group differences in Crack the Code. Taken together, these findings suggest that gifted children have better action planning, which allows them to perform better than controls in tasks that require complex problem solving and evaluation of different scenarios and solutions.