The identification of social interactions is of fundamental importance for animal behavioral studies, addressing numerous problems like investigating the influence of social hierarchical structures or the drivers of agonistic behavioral disorders. However, the majority of previous studies often rely on manual determination of the number and types of social encounters by direct observation which requires a large amount of personnel and economical efforts. To overcome this limitation and increase research efficiency and, thus, contribute to animal welfare in the long term, we propose in this study a framework for the automated identification of social contacts. In this framework, we apply a convolutional neural network (CNN) to detect the location and orientation of pigs within a video and track their movement trajectories over a period of time using a Kalman filter (KF) algorithm. Based on the tracking information, we automatically identify social contacts in the form of head–head and head–tail contacts. Moreover, by using the individual animal IDs, we construct a network of social contacts as the final output. We evaluated the performance of our framework based on two distinct test sets for pig detection and tracking. Consequently, we achieved a Sensitivity, Precision, and F1-score of 94.2%, 95.4%, and 95.1%, respectively, and a $MOTA$ score of 94.4%. The findings of this study demonstrate the effectiveness of our keypoint-based tracking-by-detection strategy and can be applied to enhance animal monitoring systems.

Faba bean (Vicia faba) is a grain legume, which is globally grown for both human consumption as well as feed for livestock. Despite its agro-ecological importance the usage of Vicia faba is severely hampered by its anti-nutritive seed-compounds vicine and convicine (V+C). The genes responsible for a low V+C content have not yet been identified. In this study, we aim to computationally identify regulatory SNPs (rSNPs), i.e., SNPs in promoter regions of genes that are deemed to govern the V+C content of Vicia faba. For this purpose we first trained a deep learning model with the gene annotations of seven related species of the Leguminosae family. Applying our model, we predicted putative promoters in a partial genome of Vicia faba that we assembled from genotyping-by-sequencing (GBS) data. Exploiting the synteny between Medicago truncatula and Vicia faba, we identified two rSNPs which are statistically significantly associated with V+C content. In particular, the allele substitutions regarding these rSNPs result in dramatic changes of the binding sites of the transcription factors (TFs) MYB4, MYB61, and SQUA. The knowledge about TFs and their rSNPs may enhance our understanding of the regulatory programs controlling V+C content of Vicia faba and could provide new hypotheses for future breeding programs.

The activity level of pigs is an important stress indicator which can be associated to tail-biting, a major issue for animal welfare of domestic pigs in conventional housing systems. Although the consideration of the animal activity could be essential to detect tail-biting before an outbreak occurs, it is often manually assessed and therefore labor intense, cost intensive and impracticable on a commercial scale. Recent advances of semi- and unsupervised convolutional neural networks (CNNs) have made them to the state of art technology for detecting anomalous behavior patterns in a variety of complex scene environments. In this study we apply such a CNN for anomaly detection to identify varying levels of activity in a multi-pen problem setup. By applying a two-stage approach we first trained the CNN to detect anomalies in the form of extreme activity behavior. Second, we trained a classifier to categorize the detected anomaly scores by learning the potential activity range of each pen. We evaluated our framework by analyzing 82 manually rated videos and achieved a success rate of 91%. Furthermore, we compared our model with a motion history image (MHI) approach and a binary image approach using two benchmark data sets, i.e., the well established pedestrian data sets published by the University of California, San Diego (UCSD) and our pig data set. The results show the effectiveness of our framework, which can be applied without the need of a labor intense manual annotation process and can be utilized for the assessment of the pig activity in a variety of applications like early warning systems to detect changes in the state of health.

Reliable animal identification in livestock husbandry is essential for various applications, including behavioral monitoring, welfare assessment, and the analysis of social structures. Although recent advancements in deep learning models have improved animal identification using biometric markers, their applicability remains limited for species without distinctive traits like pigs. Consequently, synthetic features such as ear tags have become widely adopted. However, challenges such as poor lighting conditions and the complexity of ear tag coding continue to restrict the effectiveness of Computer Vision and Deep Learning techniques. In this study, we introduce a robust, lighting-invariant method for individual pig identification that leverages commercially available ear tags within a sequential detection pipeline. Our approach employs four object detection models in succession to detect pigs, localize ear tags, perform rotation correction via pin detection, and recognize digits, ultimately generating a reliable ID proposal. In a first evaluation stage, we assessed the performance of each model independently, achieving a mAP0.95 value of 0.970, 0.979, 0.974 and 0.979 for the pig detection, ear tag detection, pin detection and ID classification model, respectively. In addition, our method was further evaluated in two different camera environments to assess its performance in both familiar and unfamiliar conditions. The results demonstrate that the proposed approach achieves a very high precision of 0.996 in a familiar top-down camera scenario and maintained a strong generalization performance in an unfamiliar, close-up setup with a precision of 0.913 and a recall of 0.903. Furthermore, by publicly proposing three custom datasets for ear tag, pin, and digit detection, we aim to support reproducibility and further research in automated animal identification for precision livestock farming. The findings of this study demonstrate the effectiveness of ID-based animal identification and the proposed method could be integrated within advanced multi-object tracking systems to enable continuous animal observation and for monitoring specific target areas, thereby significantly enhancing overall livestock management systems.

Nowadays, video monitoring of farrowing and automatic video evaluation using Deep Learning have become increasingly important in farm animal science research and open up new possibilities for addressing specific research questions like the determination of husbandry relevant indicators. A robust detection performance of newborn piglets is essential for reliably monitoring the farrowing process and to access important information about the welfare status of the sow and piglets. Although object detection algorithms are increasingly being used in various scenarios in the field of livestock farming, their usability for detecting newborn piglets has so far been limited. Challenges such as frequent animal occlusions, high overlapping rates or strong heterogeneous animal postures increase the complexity and place new demands on the detection model. Typically, new data is manually annotated to improve model performance, but the annotation effort is expensive and time-consuming. To address this problem, we propose a Noisy Student approach to automatically generate annotation information and train an improved piglet detection model. By using a teacher-student model relationship we transform the image structure and generate pseudo-labels for the object classes piglet and tail. As a result, we improve the initial detection performance of the teacher model from 0.561, 0.838, 0.672 to 0.901, 0.944, 0.922 for the performance metrics Recall, Precision and F1-score, respectively. The results of this study can be used in two ways. Firstly, the results contribute directly to the improvement of piglet detection in the context of birth monitoring systems and the evaluation of the farrowing progress. Secondly, the approach presented can be transferred to other research questions and species, thereby reducing the problem of cost-intensive annotation processes and increase training efficiency. In addition, we provide a unique dataset for the detection and evaluation of newborn piglets and sow body parts to support researchers in the task of monitoring the farrowing process.

The Beet necrotic yellow vein virus (BNYVV) causes rhizomania in sugar beet (Beta vulgaris L.), which is one of the most destructive diseases in sugar beet worldwide. In breeding projects towards resistance against BNYVV, the enzyme-linked immunosorbent assay (ELISA) is used to determine the virus concentration in plant roots and, thus, the resistance levels of genotypes. Here, we present a simulation study to generate 10,000 small samples from the estimated density functions of ELISA values from susceptible and resistant sugar beet genotypes. We apply receiver operating characteristic (ROC) analysis to these samples to optimise the cutoff values for sample sizes from two to eight and determine the false positive rates (FPR), true positive rates (TPR), and area under the curve (AUC). We present, furthermore, an alternative approach based upon Bayes factors to improve the decision procedure. The Bayesian approach has proven to be superior to the simple cutoff approach. The presented results could help evaluate or improve existing breeding programs and help design future selection procedures based upon ELISA. An R-script for the classification of sample data based upon Bayes factors is provided.