Manivannan Yegambaram

Prof. Manivannan Yegambaram

Center for Translational Science, Florida International University, Miami, Florida, USA
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Biography

I am an Assistant Professor at the Florida International University. My interest is focused on identification of post-translational modifications of proteins and metabolic reprogramming associated with pulmonary hypertension and acute lung injury. Currently, we are utilizing mass spectrometry-based approach to understand the proteomic and metabolic reprogramming in vascular cells during pulmonary hypertension development and acute lung injury. Specifically, I am understanding the role of protein post‐translational modifications (PTMs), including phosphorylation, nitration, ubiquitination, SUMOylation, O‐GlcNAcylation, methylation and acetylation, which are known to play important roles in pathological cardiac diseases. Our laboratory has demonstrated expertise in Mass spectrometry, Proteomics, Molecular Modeling and have developed unique inhibitory peptides which can be utilized to specifically target key PTM sites on the protein to either enhance or block its activity. In addition, utilizing metabolomics and fluxomics we are able to analyze snapshots of over 600 metabolites and metabolic fluxes, at a given time and conditions. To examine intracellular fluxes in detail, 13Ctracer experiments using 13C Glucose, 13C Glutamine and 13C Palmitic acid as labeled substrates are used to generate a quantitative map of cellular metabolism by assigning flux values to the reactions in the network model.

Affiliations

Florida International University

Miami, Florida, USA

Center for Translational Science

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Experience

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