This study used machine learning techniques to predict leaf nitrogen content from canopy hyperspectral data of sugarcane (Saccharum officinarum L.), thereby providing a rapid and non-invasive alternative to traditional laboratory analysis. The experiment was conducted in Piracicaba, São Paulo, Brazil, using ground-based spectroradiometry to obtain the canopy spectral signatures at different vegetative stages and under varying nitrogen doses. Spectral data were acquired at 67, 99, 144, 164, 200, 228, 255, and 313 days after cutting followed by leaf sampling and laboratory quantification of the leaf nitrogen content (LNC). Partial least squares regression, random forest, and extreme gradient boosting models were applied to predict the LNC. The relationship between the spectral response and leaf nitrogen was primarily associated with the visible and RedEdge regions, which are sensitive to chlorophyll absorption, pigment concentration, and crop nutritional status. Among the evaluated models, PLSR achieved the best performance, indicating that canopy hyperspectral sensing may be used as a complementary approach for nitrogen monitoring under field conditions; however, its predictive accuracy remained moderate. Overall, the results suggest that VIS-near-infrared region (NIR) hyperspectral data, combined with machine learning techniques, can support nitrogen monitoring in sugarcane. However, further validation under expanded field conditions is required before its implementation in operational nutrient management.